• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MSK1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177737

Description: Rabbit IgG polyclonal antibody for Ribosomal protein S6 kinase alpha-5(RPS6KA5) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.Background: Ribosomal protein S6 kinase alpha-5, also known as MSK1, is an enzyme that in humans is encoded… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MSK1, Polyclonal Antibody
Full Product Name
Anti-MSK1 Antibody
Product Gene Name
anti-MSK1 antibody
Product Synonym Names
Ribosomal protein S6 kinase alpha-5; 90 kDa ribosomal protein S6 kinase 5; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; Ribosomal protein S6 kinase alpha-5; RLPK; RPS6KA5; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
81,780 Da
OMIM
603607
3D Structure
ModBase 3D Structure for O75582
Immunogen
E Coli-derived human MSK1 recombinant protein (Position: V540-Q665). Human MSK1 shares 98% amino acid (aa) sequence identity with mouse MSK1.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MSK1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes for anti-MSK1 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunocytochemistry Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-MSK1 antibody

Anti- MSK1 Picoband antibody, MBS177737, Western blotting
All lanes: Anti MSK1 (MBS177737) at 0.5ug/ml
WB: Recombinant Human MSK1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti-MSK1 Polyclonal Antibody – Cat# MBS177737

Western Blot (WB) of anti-MSK1 antibody

Anti- MSK1 Picoband antibody, MBS177737, Western blotting
All lanes: Anti MSK1 (MBS177737) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
Anti-MSK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS177737

Immunohistochemistry (IHC) of anti-MSK1 antibody

Anti- MSK1 Picoband antibody, MBS177737, IHC(P)
IHC(P): Mouse Cardiac Muscle Tissue
Anti-MSK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177737

Immunohistochemistry (IHC) of anti-MSK1 antibody

Anti- MSK1 Picoband antibody, MBS177737, IHC(P)
IHC(P): Rat Brain Tissue
Anti-MSK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177737

Immunohistochemistry (IHC) of anti-MSK1 antibody

Anti- MSK1 Picoband antibody, MBS177737, IHC(P)
IHC(P): Human Placenta Tissue
Anti-MSK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177737

Product References and Citations for anti-MSK1 antibody

1. Deak, M., Clifton, A. D., Lucocq, J. M., Alessi, D. R. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17: 4426-4441, 1998. 2. Jiang, C., Yu, L., Tu, Q., Zhao, Y., Zhang, H., Zhao, S. Assignment of a member of the ribosomal protein S6 kinase family, RPS6KA5, to human chromosome 14q31-q32.1 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 261-261, 1999.

Other Notes

Small volumes of anti-MSK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSK1 purchase

MBS177737 is an antibody targeting MSK1. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for MSK1. It may not necessarily be applicable to this product.

NCBI GI #
32528295
NCBI GeneID
9252
NCBI Accession #
NP_004746.2
UniProt Primary Accession #
O75582
UniProt Secondary Accession #
O95316; Q96AF7; B7Z2Y5
UniProt Related Accession #
O75582
GenBank Nucleotide #
NM_004755.3
NCBI Official Full Name
ribosomal protein S6 kinase alpha-5 isoform a
NCBI Official Synonym Full Names
ribosomal protein S6 kinase A5
NCBI Official Symbol
RPS6KA5
NCBI Official Synonym Symbols
MSK1; RLPK; MSPK1
UniProt Protein Name
Ribosomal protein S6 kinase alpha-5
UniProt Synonym Protein Names
90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL
UniProt Gene Name
RPS6KA5
UniProt Synonym Gene Names
MSK1; S6K-alpha-5; RSKL
UniProt Entry Name
KS6A5_HUMAN
NCBI Protein Information
ribosomal protein S6 kinase alpha-5

UniProt Comments for MSK1

MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, AGC; AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: ATP binding; histone serine kinase activity (H3-S10 specific); magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; axon guidance; epidermal growth factor receptor signaling pathway; histone phosphorylation; inflammatory response; negative regulation of cytokine production; negative regulation of transcription, DNA-dependent; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; stimulatory C-type lectin receptor signaling pathway
Show more

Research Articles on MSK1

1. Interrupting MSK1 activation is a new target for antioxidants.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MSK1 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 C-type Lectin Receptors (CLRs) Pathway antibodies  C-type Lectin Receptors (CLRs) Pathway Diagram
 CD209 (DC-SIGN) Signaling Pathway antibodies  CD209 (DC-SIGN) Signaling Pathway Diagram
 CREB Phosphorylation Pathway antibodies  CREB Phosphorylation Pathway Diagram

Diseases associated with anti-MSK1 antibody

 Disease Name  Pubmed Publications
 Skin Neoplasms Antibodies  >1 publications with MSK1 and Skin Neoplasms
 Neoplasm Metastasis Antibodies  >1 publications with MSK1 and Neoplasm Metastasis

Organs/Tissues associated with anti-MSK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with MSK1 and Brain
 Uterus Antibodies  >1 publications with MSK1 and Uterus
 Skin Antibodies  >1 publications with MSK1 and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000