• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Nucleobindin 2 (NUCB2) Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177786

Description: Rabbit IgG polyclonal antibody for Nucleobindin-2(NUCB2) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.Background: Nucleobindin-2 is a calcium-binding EF-hand protein that in humans is encoded by the NUCB2 gene. By exon trapping and… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nucleobindin 2 (Nucb2), Polyclonal Antibody
Full Product Name
Anti-Nucleobindin 2 Antibody
Product Gene Name
anti-Nucb2 antibody
Product Synonym Names
Nucleobindin-2; DNA binding protein NEFA; DNA-binding protein NEFA; Gastric cancer antigen Zg4; NEFA; Nefastin 1; Nefastin-1; Nefastin1; Nucb2; NUCB2_HUMAN; Nucleobindin-2; Nucleobinding 2; Prepronefastin; Prepronesfatin; nucleobindin 2
Clonality
Polyclonal
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
50,304 Da
3D Structure
ModBase 3D Structure for P81117
Immunogen
E Coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Nucb2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-Nucb2 antibody
ELISA Concentration: 0.1-0.5ug/ml
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-Nucb2 antibody

Western blot analysis of Nucleobindin 2 expression in HEPA whole cell lysates (lane 1). Nucleobindin 2 at 55KD was detected using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody at0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Anti-NUCB2 Polyclonal Antibody – Cat# MBS177786

Immunohistochemistry (IHC) of anti-Nucb2 antibody

Nucleobindin 2 was detected in paraffin-embedded sections of mouse gaster tissues using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Anti-NUCB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177786

Immunohistochemistry (IHC) of anti-Nucb2 antibody

Nucleobindin 2 was detected in paraffin-embedded sections of rat gaster tissues using rabbit anti- Nucleobindin 2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Anti-NUCB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177786

Product References and Citations for anti-Nucb2 antibody

1. "Entrez Gene: NUCB2 nucleobindin 2". 2. Barnikol-Watanabe S, Gross NA, Gotz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N (February 1995). "Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein". Biol Chem Hoppe Seyler 375 (8): 497-512. 3. DeAngelis, M., Drury, S., Doucet, J., Sherry, S., Buckley, L., Pelias, M., Deininger, P., Keats, B., Batzer, M. Construction of a BAC contig and isolation of candidate genes in the Acadian Usher syndrome (USH1C) critical region. (Abstract) Am. J. Hum. Genet. 61 (suppl.): A233 only, 1997.

Other Notes

Small volumes of anti-Nucb2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nucb2 purchase

MBS177786 is an antibody targeting Nucb2. Also known as Anti-Nucleobindin 2 Antibody. Reactive in Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for Nucb2. It may not necessarily be applicable to this product.

NCBI GI #
194440700
NCBI GeneID
53322
NCBI Accession #
NP_001123951.1
UniProt Primary Accession #
P81117
UniProt Related Accession #
P81117
GenBank Nucleotide #
NM_001130479.2
NCBI Official Full Name
nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
Nucb2
NCBI Official Synonym Symbols
Nefa; Calnuc; AI607786; Nesfatin-1
UniProt Protein Name
Nucleobindin-2
UniProt Synonym Protein Names
DNA-binding protein NEFA; Prepronesfatin
Protein Family
Nucleobindin
UniProt Gene Name
Nucb2
UniProt Synonym Gene Names
Nefa
UniProt Entry Name
NUCB2_MOUSE
NCBI Protein Information
nucleobindin-2

UniProt Comments for Nucb2

NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Vesicle; Secreted, signal peptide

Cellular Component: cytoplasm; endoplasmic reticulum; ER-Golgi intermediate compartment; extracellular region; extracellular space; Golgi apparatus; Golgi medial cisterna; membrane; nuclear outer membrane; nucleus

Molecular Function: calcium ion binding; DNA binding; metal ion binding; tumor necrosis factor receptor binding

Biological Process: cellular calcium ion homeostasis; negative regulation of appetite; negative regulation of cAMP biosynthetic process; negative regulation of fat cell differentiation; negative regulation of insulin receptor signaling pathway; positive regulation of fatty acid oxidation
Show more

Research Articles on Nucb2

1. MGN3-1 cells respond differently to nutrients in secreting NUCB2/nesfatin-1 and ghrelin. Similarly, acute or chronic intake of nutrients does influence NUCB2 mRNA expression and NUCB2/nesfatin-1 release in a diet specific manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Nucb2 antibody

 Reference Product  PubMed Publications
 Npy antibody  >3 publications with Nucb2 and Npy
 Erap1 antibody  >2 publications with Nucb2 and Erap1

Diseases associated with anti-Nucb2 antibody

 Disease Name  Pubmed Publications
 Weight Gain Antibodies  >13 publications with Nucb2 and Weight Gain
 Insulin Resistance Antibodies  >9 publications with Nucb2 and Insulin Resistance
 Inflammation Antibodies  >6 publications with Nucb2 and Inflammation
 Carcinoma Antibodies  >5 publications with Nucb2 and Carcinoma
 Liver Diseases Antibodies  >4 publications with Nucb2 and Liver Diseases
 Necrosis Antibodies  >4 publications with Nucb2 and Necrosis
 Breast Neoplasms Antibodies  >4 publications with Nucb2 and Breast Neoplasms
 Disease Models, Animal Antibodies  >4 publications with Nucb2 and Disease Models, Animal
 Hypertension Antibodies  >3 publications with Nucb2 and Hypertension
 Prostatic Neoplasms Antibodies  >3 publications with Nucb2 and Prostatic Neoplasms

Organs/Tissues associated with anti-Nucb2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >78 publications with Nucb2 and Brain
 Blood Antibodies  >37 publications with Nucb2 and Blood
 Pancreas Antibodies  >23 publications with Nucb2 and Pancreas
 Liver Antibodies  >20 publications with Nucb2 and Liver
 Ovary Antibodies  >9 publications with Nucb2 and Ovary
 Muscle Antibodies  >8 publications with Nucb2 and Muscle
 Pituitary Gland Antibodies  >8 publications with Nucb2 and Pituitary Gland
 Connective Tissue Antibodies  >6 publications with Nucb2 and Connective Tissue
 Testis Antibodies  >6 publications with Nucb2 and Testis
 Prostate Antibodies  >4 publications with Nucb2 and Prostate
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000