Western blot analysis of TRIO expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from rat brain and HepG2, using TRIO Antibody.
Western blot analysis of lysates from Mouse brain, using TRIO Ab. The lane on the left was treated with blocking peptide
MBS9604199 at 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.
MBS9604199 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (1:200) and mouse anti-beta tubulin Ab (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).
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