Granzymes A (GZMS-A) ELISA Kit for Rat

Name: Rat GZMS-A ELISA Kit; Granzymes A; MyBioSource
Brand: MyBioSource
SKU: MBS017784
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS017784

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of GZMA (hereafter termed "analyte") in… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Granzymes A (GZMS-A), ELISA Kit

Full Product Name

Rat Granzymes A ELISA Kit

Product Gene Name

GZMS-A elisa kit

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Detection Range

0.25ng/ml-8ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

28,999 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (54398474..54406080). Location: 5q11-q12

OMIM

140050

3D Structure

ModBase 3D Structure for P12544

Other Notes

Small volumes of GZMS-A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMS-A purchase

MBS017784 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Granzymes A (GZMS-A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GZMS-A. The ELISA analytical biochemical technique of the MBS017784 kit is based on GZMS-A antibody-GZMS-A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMS-A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMS-A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GZMS-A. It may not necessarily be applicable to this product.

NCBI GI #

5453676

NCBI GeneID

3001

NCBI Accession #

NP_006135.1

UniProt Primary Accession #

P12544

UniProt Secondary Accession #

Q6IB36; A4PHN1

UniProt Related Accession #

P12544

GenBank Nucleotide #

NM_006144.3

NCBI Official Full Name

granzyme A

NCBI Official Synonym Full Names

granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)

NCBI Official Symbol

GZMA

NCBI Official Synonym Symbols

HFSP; CTLA3

UniProt Protein Name

Granzyme A

UniProt Synonym Protein Names

CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor

UniProt Gene Name

GZMA

UniProt Synonym Gene Names

CTLA3; HFSP; H factor; HF

UniProt Entry Name

GRAA_HUMAN

NCBI Protein Information

granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)

NCBI Summary for GZMS-A

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008]

UniProt Comments for GZMS-A

GZMA: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Homodimer; disulfide-linked. Interacts with APEX1. Dexamethasone (DEX) induces expression of isoform beta and represses expression of isoform alpha. The alteration in expression is mediated by binding of glucocorticoid receptor to independent promoters adjacent to the alternative first exons of isoform alpha and isoform beta. Belongs to the peptidase S1 family. Granzyme subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.78; Secreted; Secreted, signal peptide; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 5q11-q12

Cellular Component: extracellular region; immunological synapse; nucleus

Molecular Function: protein binding; protein homodimerization activity; serine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; positive regulation of apoptosis; apoptosis; cytolysis; negative regulation of oxidoreductase activity; negative regulation of endodeoxyribonuclease activity; immune response; negative regulation of DNA binding

Research Articles on GZMS-A

1. Patients with chronic immune thrombocytopenia have elevated serum granzyme A levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GZMS-A elisa kit

Reference Product	PubMed Publications
PRF1 elisa kit	>140 publications with GZMS-A and PRF1
GZMK elisa kit	>11 publications with GZMS-A and GZMK
GNLY elisa kit	>6 publications with GZMS-A and GNLY
IL2 elisa kit	>2 publications with GZMS-A and IL2

Pathways associated with GZMS-A elisa kit

Products by Pathway	Pathway Diagram
IL12-mediated Signaling Events Pathway antibodies	IL12-mediated Signaling Events Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with GZMS-A elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>32 publications with GZMS-A and Necrosis
Inflammation Antibodies	>15 publications with GZMS-A and Inflammation
Disease Models, Animal Antibodies	>7 publications with GZMS-A and Disease Models, Animal
Drug Toxicity Antibodies	>5 publications with GZMS-A and Drug Toxicity
Hypersensitivity Antibodies	>5 publications with GZMS-A and Hypersensitivity
Gastrointestinal Diseases Antibodies	>5 publications with GZMS-A and Gastrointestinal Diseases
Lung Diseases Antibodies	>4 publications with GZMS-A and Lung Diseases
Blood Coagulation Disorders Antibodies	>3 publications with GZMS-A and Blood Coagulation Disorders
Liver Diseases Antibodies	>3 publications with GZMS-A and Liver Diseases
Kidney Diseases Antibodies	>2 publications with GZMS-A and Kidney Diseases

Organs/Tissues associated with GZMS-A elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>261 publications with GZMS-A and Blood
Lung Antibodies	>10 publications with GZMS-A and Lung
Skin Antibodies	>10 publications with GZMS-A and Skin
Spleen Antibodies	>9 publications with GZMS-A and Spleen
Kidney Antibodies	>9 publications with GZMS-A and Kidney
Heart Antibodies	>8 publications with GZMS-A and Heart
Intestine Antibodies	>8 publications with GZMS-A and Intestine
Uterus Antibodies	>5 publications with GZMS-A and Uterus
Lymph Node Antibodies	>5 publications with GZMS-A and Lymph Node
Brain Antibodies	>3 publications with GZMS-A and Brain