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MAD1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177892

Description: Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD1(MAD1L1) detection. Tested with WB, IHC-P in Human;Mouse;RatBackground: Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by… More details

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Product Overview

Online Datasheet in PDF
Product Name
MAD1, Polyclonal Antibody
Full Product Name
Anti-MAD1 Antibody
Product Gene Name
anti-MAD1 antibody
Product Synonym Names
Mitotic spindle assembly checkpoint protein MAD1; hMAD1; HsMAD1; MAD1; MAD1 mitotic arrest deficient like 1 (yeast); MAD1-like protein 1; MAD1L1; MD1L1_HUMAN; Mitotic arrest deficient 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Mitotic spindle assembly checkpoint protein MAD1; PIG9; Tax binding protein 181; Tax-binding protein 181; TP53I9; Tumor protein p53 inducible protein 9; TXBP181; MAD1 mitotic arrest deficient-like 1 (yeast)
Clonality
Polyclonal
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
72,286 Da
OMIM
602686
3D Structure
ModBase 3D Structure for Q9Y6D9
Immunogen
E Coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MAD1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-MAD1 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-MAD1 antibody

Anti- MAD1 Picoband antibody, MBS177892, Western blotting
All lanes: Anti MAD1 (MBS177892) at 0.5ug/ml
WB: Recombinant Human MAD1 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti-MAD1 Polyclonal Antibody – Cat# MBS177892

Western Blot (WB) of anti-MAD1 antibody

Anti- MAD1 Picoband antibody, MBS177892, Western blotting
All lanes: Anti MAD1 (MBS177892) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: SHG Whole Cell Lysate at 40ug
Lane 6: 22RV1 Whole Cell Lysate at 40ug
Lane 7: PANC Whole Cell Lysate at 40ug
Predicted bind size: 83KD
Observed bind size: 83KD
Anti-MAD1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS177892

Immunohistochemistry (IHC) of anti-MAD1 antibody

Anti- MAD1 Picoband antibody, MBS177892, IHC(P)
IHC(P): Human Placenta Tissue
Anti-MAD1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177892

Product References and Citations for anti-MAD1 antibody

1. "Entrez Gene: MAD1L1 MAD1 mitotic arrest deficient-like 1 (yeast)" 2. Jin, D.-Y., Kozak, C. A., Pangilinan, F., Spencer, F., Green, E. D., Jeang, K.-T. Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5. Genomics 55: 363-364, 1999. 3. Jin, D.-Y., Spencer, F., Jeang, K.-T. Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Cell 93: 81-91, 1998. 4. Tsukasaki, K., Miller, C. W., Greenspun, E., Eshaghian, S., Kawabata, H., Fujimoto, T., Tomonaga, M., Sawyers, C., Said, J. W., Koeffler, H. P. Mutations in the mitotic check point gene, MAD1L1, in human cancers. Oncogene 20: 3301-3305, 2001.

Other Notes

Small volumes of anti-MAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAD1 purchase

MBS177892 is an antibody targeting MAD1. Reactive in Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for MAD1. It may not necessarily be applicable to this product.

NCBI GI #
62243369
NCBI GeneID
8379
NCBI Accession #
NP_001013858.1
UniProt Primary Accession #
Q9Y6D9
UniProt Secondary Accession #
Q13312; Q75MI0; Q86UM4; Q9UNH0; B3KR41
UniProt Related Accession #
Q9Y6D9
GenBank Nucleotide #
NM_001013836.1
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD1 isoform a
NCBI Official Synonym Full Names
MAD1 mitotic arrest deficient like 1
NCBI Official Symbol
MAD1L1
NCBI Official Synonym Symbols
MAD1; PIG9; TP53I9; TXBP181
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD1
UniProt Synonym Protein Names
Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181
Protein Family
Mitotic spindle checkpoint protein
UniProt Gene Name
MAD1L1
UniProt Synonym Gene Names
MAD1; TXBP181; MAD1-like protein 1; HsMAD1; hMAD1
UniProt Entry Name
MD1L1_HUMAN
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD1

NCBI Summary for MAD1

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
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UniProt Comments for MAD1

MAD1L1: a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Expressed weakly at G0/G1 and highly at late S and G2/M phase. Induced by p53. Becomes hyperphosphorylated in late S through M phases or after mitotic spindle damage. Two alternatively spliced isoforms have been described. Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

Protein type: Cytoskeletal; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: actin cytoskeleton; centrosome; cytoplasm; cytosol; kinetochore; nuclear pore; nucleus; spindle; spindle pole

Molecular Function: protein binding

Biological Process: cell division; mitosis; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of T cell proliferation; sister chromatid cohesion; thymus development

Disease: Prostate Cancer
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Research Articles on MAD1

1. MAD1L1 rs12666575 polymorphism may play a protective role against schizophrenia (SCZ) in the Chinese population. rs12666575 may be associated with general psychopathology and thought disturbance in SCZ patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAD1 antibody

 Reference Product  PubMed Publications
 MAD2L1 antibody  >64 publications with MAD1 and MAD2L1
 BUB1 antibody  >33 publications with MAD1 and BUB1
 CDC20 antibody  >31 publications with MAD1 and CDC20
 BUB3 antibody  >18 publications with MAD1 and BUB3
 TTK antibody  >13 publications with MAD1 and TTK
 NDC80 antibody  >5 publications with MAD1 and NDC80
 BUB1B antibody  >5 publications with MAD1 and BUB1B
 CENPE antibody  >3 publications with MAD1 and CENPE
 ZW10 antibody  >3 publications with MAD1 and ZW10

Pathways associated with anti-MAD1 antibody

 Products by Pathway  Pathway Diagram
 Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway antibodies  Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway Diagram
 Amplification Of Signal From The Kinetochores Pathway antibodies  Amplification Of Signal From The Kinetochores Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram

Diseases associated with anti-MAD1 antibody

 Disease Name  Pubmed Publications
 Chromosome Aberrations Antibodies  >16 publications with MAD1 and Chromosome Aberrations
 Leukemia Antibodies  >14 publications with MAD1 and Leukemia
 Adenocarcinoma Antibodies  >8 publications with MAD1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >7 publications with MAD1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >6 publications with MAD1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >6 publications with MAD1 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >6 publications with MAD1 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >4 publications with MAD1 and Carcinoma, Hepatocellular
 Necrosis Antibodies  >4 publications with MAD1 and Necrosis
 Disease Models, Animal Antibodies  >4 publications with MAD1 and Disease Models, Animal

Organs/Tissues associated with anti-MAD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with MAD1 and Blood
 Bone Antibodies  >9 publications with MAD1 and Bone
 Lung Antibodies  >9 publications with MAD1 and Lung
 Bone Marrow Antibodies  >6 publications with MAD1 and Bone Marrow
 Stomach Antibodies  >5 publications with MAD1 and Stomach
 Liver Antibodies  >4 publications with MAD1 and Liver
 Skin Antibodies  >4 publications with MAD1 and Skin
 Prostate Antibodies  >3 publications with MAD1 and Prostate
 Thymus Antibodies  >2 publications with MAD1 and Thymus
 Lymph Node Antibodies  >2 publications with MAD1 and Lymph Node
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