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CARD4 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177898

Description: Rabbit IgG polyclonal antibody for Nucleotide-binding oligomerization domain-containing protein 1(NOD1) detection. Tested with WB, IHC-P in Human;Rat.Background: Nucleotide-binding oligomerization domain-containing protein 1, also known as CARD4,… More details

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Product Overview

Online Datasheet in PDF
Product Name
CARD4, Polyclonal Antibody
Full Product Name
Anti-CARD4 Antibody
Product Gene Name
anti-CARD4 antibody
Product Synonym Names
Nucleotide-binding oligomerization domain-containing protein 1; CARD 4; CARD4; Caspase recruitment domain 4; Caspase recruitment domain containing protein 4; Caspase recruitment domain family member 4; Caspase recruitment domain protein 4; Caspase recruitment domain-containing protein 4; CLR 7.1; CLR7.1; NLR family CARD domain containing 1; NLRC 1; NLRC1; NOD 1; Nod1; NOD1 protein; NOD1_HUMAN; Nucleotide binding oligomerization domain containing 1; Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1; Nucleotide-binding oligomerization domain-containing protein 1; Protein Nod1; nucleotide-binding oligomerization domain containing 1
Clonality
Polyclonal
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
27,949 Da
OMIM
605980
3D Structure
ModBase 3D Structure for Q9Y239
Immunogen
E Coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CARD4 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CARD4 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-CARD4 antibody

Figure 1. Western blot analysis of CARD4 using anti-CARD4 antibody (MBS177898).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 Whole Cell Lysate,
Lane 2: Rat Cardiac Muscle Tissue Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CARD4 at approximately 107KD. The expected band size for CARD4 is at 107KD.
Anti-CARD4 Polyclonal Antibody – Cat# MBS177898

Immunohistochemistry (IHC) of anti-CARD4 antibody

Figure 2. IHC analysis of CARD4 using anti-CARD4 antibody (MBS177898).
CARD4 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CARD4 Antibody (MBS177898) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-CARD4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177898

Immunohistochemistry (IHC) of anti-CARD4 antibody

Figure 3. IHC analysis of CARD4 using anti-CARD4 antibody (MBS177898).
CARD4 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CARD4 Antibody (MBS177898) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-CARD4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177898

Immunohistochemistry (IHC) of anti-CARD4 antibody

Figure 4. IHC analysis of CARD4 using anti-CARD4 antibody (MBS177898).
CARD4 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CARD4 Antibody (MBS177898) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-CARD4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177898

Immunocytochemistry (ICC) of anti-CARD4 antibody

Figure 5. IHC analysis of CARD4 using anti-CARD4 antibody (MBS177898).
CARD4 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CARD4 Antibody (MBS177898) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-CARD4 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS177898

Product References and Citations for anti-CARD4 antibody

1. Chamaillard, M., Hashimoto, M., Horie, Y., Masumoto, J., Su, Q., Saab, L., Ogura, Y., Kawasaki, A., Fukase, K., Kusumoto, S., Valvano, M. A., Foster, S. J., Mak, T. W., Nunez, G., Inohara, N. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nature Immun. 4: 702-707, 2003. 2. da Silva Correia, J., Miranda, Y., Austin-Brown, N., Hsu, J., Mathison, J., Xiang, R., Zhou, H., Li, Q., Han, J., Ulevitch, R. J. Nod1-dependent control of tumor growth. Proc. Nat. Acad. Sci. 103: 1840-1845, 2006. 3. Martinon F, Tschopp J (2006). "NLRs join TLRs as innate sensors of pathogens.".Trends Immunol. 26 (8): 447-54.

Other Notes

Small volumes of anti-CARD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CARD4 purchase

MBS177898 is an antibody targeting CARD4. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CARD4. It may not necessarily be applicable to this product.

NCBI GI #
5174617
NCBI GeneID
10392
NCBI Accession #
NP_006083.1
UniProt Primary Accession #
Q9Y239
UniProt Secondary Accession #
Q549U4; Q8IWF5; B4DTU3
UniProt Related Accession #
Q9Y239
GenBank Nucleotide #
NM_006092.2
NCBI Official Full Name
nucleotide-binding oligomerization domain-containing protein 1
NCBI Official Synonym Full Names
nucleotide binding oligomerization domain containing 1
NCBI Official Symbol
NOD1
NCBI Official Synonym Symbols
CARD4; NLRC1; CLR7.1
UniProt Protein Name
Nucleotide-binding oligomerization domain-containing protein 1
UniProt Synonym Protein Names
Caspase recruitment domain-containing protein 4
UniProt Gene Name
NOD1
UniProt Synonym Gene Names
CARD4
UniProt Entry Name
NOD1_HUMAN
NCBI Protein Information
nucleotide-binding oligomerization domain-containing protein 1

NCBI Summary for CARD4

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Oct 2009]
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UniProt Comments for CARD4

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasm; cytosol

Molecular Function: ATP binding; CARD domain binding; caspase activator activity; identical protein binding; peptidoglycan binding; protein binding; protein homodimerization activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response; defense response to bacterium; defense response to Gram-positive bacterium; detection of bacterium; detection of biotic stimulus; inflammatory response; innate immune response; interleukin-8 biosynthetic process; JNK cascade; positive regulation of caspase activity; positive regulation of dendritic cell antigen processing and presentation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta production; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of nitric-oxide synthase activity; positive regulation of tumor necrosis factor production; protein oligomerization; signal transduction
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Research Articles on CARD4

1. NOD1 and NOD2, two members of the NOD-like receptor family of pattern recognition receptors, are important mediators of ER-stress-induced inflammation in mouse and human cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CARD4 antibody

 Reference Product  PubMed Publications
 RIPK2 antibody  >74 publications with CARD4 and RIPK2
 CASP1 antibody  >48 publications with CARD4 and CASP1
 ATG16L1 antibody  >11 publications with CARD4 and ATG16L1
 CASP9 antibody  >7 publications with CARD4 and CASP9
 CASP8 antibody  >4 publications with CARD4 and CASP8
 CARD6 antibody  >3 publications with CARD4 and CARD6

Pathways associated with anti-CARD4 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram
 Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway antibodies  Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interleukin-1 Signaling Pathway antibodies  Interleukin-1 Signaling Pathway Diagram
 JNK (c-Jun Kinases) Phosphorylation And Activation Mediated By Activated Human TAK1 Pathway antibodies  JNK (c-Jun Kinases) Phosphorylation And Activation Mediated By Activated Human TAK1 Pathway Diagram
 MAP Kinase Activation In TLR Cascade Pathway antibodies  MAP Kinase Activation In TLR Cascade Pathway Diagram
 MyD88 Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88 Cascade Initiated On Plasma Membrane Pathway Diagram

Diseases associated with anti-CARD4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >256 publications with CARD4 and Inflammation
 Necrosis Antibodies  >87 publications with CARD4 and Necrosis
 Lung Diseases Antibodies  >42 publications with CARD4 and Lung Diseases
 Disease Models, Animal Antibodies  >39 publications with CARD4 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >21 publications with CARD4 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >16 publications with CARD4 and Nervous System Diseases
 Carcinoma Antibodies  >12 publications with CARD4 and Carcinoma
 Liver Diseases Antibodies  >12 publications with CARD4 and Liver Diseases
 Kidney Diseases Antibodies  >8 publications with CARD4 and Kidney Diseases
 Fibrosis Antibodies  >7 publications with CARD4 and Fibrosis

Organs/Tissues associated with anti-CARD4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >168 publications with CARD4 and Blood
 Intestine Antibodies  >66 publications with CARD4 and Intestine
 Lung Antibodies  >52 publications with CARD4 and Lung
 Bone Antibodies  >42 publications with CARD4 and Bone
 Liver Antibodies  >30 publications with CARD4 and Liver
 Kidney Antibodies  >30 publications with CARD4 and Kidney
 Spleen Antibodies  >29 publications with CARD4 and Spleen
 Mouth Antibodies  >26 publications with CARD4 and Mouth
 Vascular Antibodies  >24 publications with CARD4 and Vascular
 Muscle Antibodies  >15 publications with CARD4 and Muscle
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