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EME1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177915

Description: Rabbit IgG polyclonal antibody for Crossover junction endonuclease EME1(EME1) detection. Tested with WB, IHC-P in Human;Rat.Background: Crossover junction endonuclease EME1 is an enzyme that in humans is encoded by the EME1 gene. It is mapped to… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
EME1, Polyclonal Antibody
Full Product Name
Anti-EME1 Antibody
Product Gene Name
Product Synonym Names
Crossover junction endonuclease EME1; 6820428D13; Crossover junction endonuclease EME1; EC 3.1.22.-; EME1; Eme1 essential meiotic endonuclease 1 homolog 1 (S. pombe); EME1, S. pombe, homolog of, 1; EME1_HUMAN; essential meiotic endonuclease 1 homolog 1 (S pombe); Essential Meiotic Endonuclease 1 Homolog 1; essential meiotic endonuclease 1 homolog 2; Essential meiotic endonuclease 1, S. pombe, homolog of, 1; FLJ31364; hMMS4; homolog of yeast EME1 endonuclease; MGC106543; MMS4; MMS4 homolog; MMS4L; essential meiotic structure-specific endonuclease 1
Clonality
Polyclonal
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
64,779 Da
OMIM
610885
3D Structure
ModBase 3D Structure for Q96AY2
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human EME1 (520-561aa DKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQ), different from the related mouse sequence by five amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EME1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-EME1 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-EME1 antibody

Anti- EME1 Picoband antibody, MBS177915, Western blotting
All lanes: Anti EME1 (MBS177915) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: HUT Whole Cell Lysate at 40ug
Predicted bind size: 62KD
Observed bind size: 62KD
Anti-EME1 Polyclonal Antibody – Cat# MBS177915

Immunohistochemistry (IHC) of anti-EME1 antibody

Anti- EME1 Picoband antibody, MBS177915,IHC(P)
IHC(P): Rat Intestine Tissue
Anti-EME1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177915

Immunohistochemistry (IHC) of anti-EME1 antibody

Anti- EME1 Picoband antibody, MBS177915,IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-EME1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177915

Product References and Citations for anti-EME1 antibody

1. "Entrez Gene: EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe)". 2. Abraham, J., Lemmers, B., Hande, M. P., Moynahan, M. E., Chahwan, C., Ciccia, A., Essers, J., Hanada, K., Chahwan, R., Khaw, A. K., McPherson, P., Shehabeldin, A., Laister, R., Arrowsmith, C., Kanaar, R., West, S. C., Jasin, M., Hakem, R. Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells. EMBO J. 22: 6137-6147, 2003. 3. Ciccia A, Constantinou A, West SC (Jun 2003). "Identification and characterization of the human mus81-eme1 endonuclease". J Biol Chem 278 (27): 25172-8.

Other Notes

Small volumes of anti-EME1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EME1 purchase

MBS177915 is an antibody targeting EME1. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for EME1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
crossover junction endonuclease EME1 isoform 1
NCBI Official Synonym Full Names
essential meiotic structure-specific endonuclease 1
NCBI Official Symbol
EME1
NCBI Official Synonym Symbols
MMS4L; SLX2A
UniProt Protein Name
Crossover junction endonuclease EME1
UniProt Synonym Protein Names
MMS4 homolog; hMMS4
UniProt Gene Name
EME1
UniProt Synonym Gene Names
MMS4; hMMS4
UniProt Entry Name
EME1_HUMAN
NCBI Protein Information
crossover junction endonuclease EME1

NCBI Summary for EME1

This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]
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UniProt Comments for EME1

EME1: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. Belongs to the EME1/MMS4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; Nucleolus; EC 3.1.22.-

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: cytoplasm; nuclear heterochromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: DNA binding; endodeoxyribonuclease activity; metal ion binding; protein binding

Biological Process: DNA recombination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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