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AIM2 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177917

Description: Rabbit IgG polyclonal antibody for Interferon-inducible protein AIM2(AIM2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.Background: Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2, is… More details

$475 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
AIM2, Polyclonal Antibody
Full Product Name
Anti-AIM2 Antibody
Product Gene Name
anti-AIM2 antibody
Product Synonym Names
Interferon-inducible protein AIM2; Absent in melanoma 2; AIM 2; Aim2; AIM2_HUMAN; Interferon-inducible protein AIM2; OTTHUMP00000035296; PYHIN4; absent in melanoma 2
Clonality
Polyclonal
Isotype
Rabbit IgG
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
604578
3D Structure
ModBase 3D Structure for O14862
Immunogen
E Coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.
Reconstitution
0.2mL of distilled water will yield a concentration of 500ug/mL
Relevant Detection Systems
MBS recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (please inquire) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (please inquire) for IHC(P).
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-AIM2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-AIM2 antibody
WB: Concentration: 0.1-0.5ug/ml ; Tested Species: Human , Mouse
IHC-p: Concentration: 0.5-1ug/ml ; Tested Species: Human , Mouse, Rat ; Antigen Retrieval: By Heat.

Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by the end user.

Western Blot (WB) of anti-AIM2 antibody

Western blot analysis of AIM2 using anti-AIM2 antibody (MBS177917).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIM2 antigen affinity purified polyclonal antibody (Catalog # MBS177917) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # please inquire) with Tanon 5200 system. A specific band was detected for AIM2 at approximately 45 kDa. The expected band size for AIM2 is at 39,40-45 kDa.
Anti-AIM2 Polyclonal Antibody – Cat# MBS177917

Immunohistochemistry (IHC) of anti-AIM2 antibody

IHC analysis of AIM2 using anti-AIM2 antibody (MBS177917).AIM2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-AIM2 Antibody (MBS177917) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # please inquire) with DAB as the chromogen.
Anti-AIM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177917

Immunohistochemistry (IHC) of anti-AIM2 antibody

IHC analysis of AIM2 using anti-AIM2 antibody (MBS177917).AIM2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-AIM2 Antibody (MBS177917) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # please inquire) with DAB as the chromogen.
Anti-AIM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177917

Immunohistochemistry (IHC) of anti-AIM2 antibody

IHC analysis of AIM2 using anti-AIM2 antibody (MBS177917).AIM2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 ug/ml rabbit anti-AIM2 Antibody (MBS177917) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # please inquire) with DAB as the chromogen.
Anti-AIM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177917

Product References and Citations for anti-AIM2 antibody

1. DeYoung KL, Ray ME, Su YA, Anzick SL, Johnstone RW, Trapani JA, Meltzer PS, Trent JM (Aug 1997). "Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma". Oncogene 15 (4): 453-7. 2. "Entrez Gene: AIM2 absent in melanoma 2". 3. Woerner SM, Kloor M, Schwitalle Y, Youmans H, von Knebel Doeberitz M, Gebert J, Dihlmann S (2007). "The putative tumor suppressor AIM2 is frequently affected by different genetic alterations in microsatellite unstable colon cancers". Genes Chromosomes Cancer 46 (12): 1080-9.

Other Notes

Small volumes of anti-AIM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AIM2 purchase

MBS177917 is an antibody targeting AIM2. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for AIM2. It may not necessarily be applicable to this product.

NCBI GI #
4757734
NCBI GeneID
9447
NCBI Accession #
NP_004824.1
UniProt Primary Accession #
O14862
UniProt Secondary Accession #
Q5T3V9; Q96FG9; A8K7M7
UniProt Related Accession #
O14862
GenBank Nucleotide #
NM_004833.1
NCBI Official Full Name
interferon-inducible protein AIM2
NCBI Official Synonym Full Names
absent in melanoma 2
NCBI Official Symbol
AIM2
NCBI Official Synonym Symbols
PYHIN4
UniProt Protein Name
Interferon-inducible protein AIM2
UniProt Synonym Protein Names
Absent in melanoma 2
Protein Family
Interferon-inducible protein
UniProt Gene Name
AIM2
UniProt Entry Name
AIM2_HUMAN
NCBI Protein Information
interferon-inducible protein AIM2

NCBI Summary for AIM2

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq, Jul 2008]

UniProt Comments for AIM2

AIM2: a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Induced by interferon gamma. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. AIM2 and various NOD-like receptors form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1beta.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: double-stranded DNA binding; identical protein binding; protein binding

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; immune response; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; interleukin-1 beta secretion; positive regulation of defense response to virus by host; positive regulation of interleukin-1 beta production; positive regulation of interleukin-1 beta secretion; positive regulation of protein oligomerization; tumor necrosis factor-mediated signaling pathway
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Research Articles on AIM2

1. pyrin domain drives both filament formation and dsDNA binding

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AIM2 antibody

 Reference Product  PubMed Publications
 CASP1 antibody  >226 publications with AIM2 and CASP1
 NLRP3 antibody  >208 publications with AIM2 and NLRP3
 NLRC4 antibody  >94 publications with AIM2 and NLRC4
 NLRP1 antibody  >53 publications with AIM2 and NLRP1
 PYCARD antibody  >40 publications with AIM2 and PYCARD
 DDX58 antibody  >6 publications with AIM2 and DDX58
 EIF2AK2 antibody  >2 publications with AIM2 and EIF2AK2

Pathways associated with anti-AIM2 antibody

 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 NOD Pathway antibodies  NOD Pathway Diagram
 Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways antibodies  Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways Diagram
 The AIM2 Inflammasome Pathway antibodies  The AIM2 Inflammasome Pathway Diagram

Diseases associated with anti-AIM2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >144 publications with AIM2 and Inflammation
 Necrosis Antibodies  >23 publications with AIM2 and Necrosis
 Disease Models, Animal Antibodies  >22 publications with AIM2 and Disease Models, Animal
 Nervous System Diseases Antibodies  >13 publications with AIM2 and Nervous System Diseases
 Atherosclerosis Antibodies  >11 publications with AIM2 and Atherosclerosis
 Lung Diseases Antibodies  >9 publications with AIM2 and Lung Diseases
 Pneumonia Antibodies  >6 publications with AIM2 and Pneumonia
 Breast Neoplasms Antibodies  >5 publications with AIM2 and Breast Neoplasms
 Liver Cirrhosis Antibodies  >5 publications with AIM2 and Liver Cirrhosis
 Fibrosis Antibodies  >5 publications with AIM2 and Fibrosis

Organs/Tissues associated with anti-AIM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >52 publications with AIM2 and Blood
 Bone Antibodies  >27 publications with AIM2 and Bone
 Lung Antibodies  >20 publications with AIM2 and Lung
 Brain Antibodies  >19 publications with AIM2 and Brain
 Intestine Antibodies  >11 publications with AIM2 and Intestine
 Mouth Antibodies  >9 publications with AIM2 and Mouth
 Lymph Node Antibodies  >5 publications with AIM2 and Lymph Node
 Spleen Antibodies  >4 publications with AIM2 and Spleen
 Connective Tissue Antibodies  >2 publications with AIM2 and Connective Tissue
 Cervix Antibodies  >1 publications with AIM2 and Cervix
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