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RACK1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177929

Description: Rabbit IgG polyclonal antibody for Guanine nucleotide-binding protein subunit beta-2-like 1(GNB2L1) detection. Tested with WB in Human;Mouse;Rat.Background: Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1 is a 32kDa… More details

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Product Overview

Online Datasheet in PDF
Product Name
RACK1, Polyclonal Antibody
Full Product Name
Anti-RACK1 Antibody
Product Gene Name
anti-RACK1 antibody
Product Synonym Names
Guanine nucleotide-binding protein subunit beta-2-like 1; Cell proliferation-inducing gene 21 protein; GBLP_HUMAN; Gnb2 rs1; Gnb2-rs1; GNB2L1; Gnb2rs1; Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1; Guanine nucleotide binding protein beta polypeptide 2 like 1; Guanine nucleotide binding protein beta subunit 2 like 1; Guanine nucleotide binding protein beta subunit like protein 12.3; Guanine nucleotide binding protein subunit beta 2 like 1; Guanine nucleotide binding protein subunit beta like protein 12.3 antibodyGuanine nucleotide-binding protein subunit beta-2-like 1; Guanine nucleotide-binding protein subunit beta-like protein 12.3; H12.3; HLC 7; HLC-7; HLC7; Human lung cancer oncogene 7 protein; Lung cancer oncogene 7; OTTHUMP00000223704; OTTHUMP00000223870; OTTHUMP00000223891; OTTHUMP00000223893; OTTHUMP00000223900; OTTHUMP00000223902; OTTHUMP00000223930; OTTHUMP00000223931; PIG 21; PIG21; Proliferation inducing gene 21; Protein homologous to chicken B complex protein guanine nucleotide binding; RACK 1; RACK1; Receptor for activated C kinase; Receptor for activated C kinase 1; Receptor of activated protein kinase C 1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
35,077 Da
OMIM
176981
3D Structure
ModBase 3D Structure for P63244
Immunogen
E Coli-derived human RACK1 recombinant protein (Position: T2-R317). Human RACK1 shares 100% amino acid (aa) sequence identity with both mouse and rat RACK1.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RACK1 antibody
Western Blot (WB)
Application Notes for anti-RACK1 antibody
Western Blot Concentration: 0.1-0.5ug/ml

Western Blot (WB) of anti-RACK1 antibody

Anti- RACK1 antibody, MBS177929, Western blotting
All lanes: Anti RACK1 (MBS177929) at 0.5ug/ml
WB: Recombinant Human RACK1 Protein 0.5ng
Predicted bind size: 35KD
Observed bind size: 35KD
Anti-RACK1 Polyclonal Antibody – Cat# MBS177929

Western Blot (WB) of anti-RACK1 antibody

Anti- RACK1 antibody, MBS177929, Western blotting
All lanes: Anti RACK1 (MBS177929) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Lane 6: HEPA Whole Cell Lysate at 40ug
Lane 7: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
Anti-RACK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS177929

Product References and Citations for anti-RACK1 antibody

1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Patterson, R. L., van Rossum, D. B., Barrow, R. K., Snyder, S. H. RACK1 binds to inositol 1,4,5-triphosphate receptors and mediates Ca(2+) release. Proc. Nat. Acad. Sci. 101: 2328-2332, 2004. 3. Robles, M. S., Boyault, C., Knutti, D., Padmanabhan, K., Weitz, C. J. Identification of RACK1 and protein kinase C-alpha as integral components of the mammalian circadian clock. Science 327: 463-466, 2010.

Other Notes

Small volumes of anti-RACK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RACK1 purchase

MBS177929 is an antibody targeting RACK1. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for RACK1. It may not necessarily be applicable to this product.

NCBI GI #
5174447
NCBI GeneID
10399
NCBI Accession #
NP_006089.1
UniProt Primary Accession #
P63244
UniProt Secondary Accession #
P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
UniProt Related Accession #
P63244
GenBank Nucleotide #
NM_006098.4
NCBI Official Full Name
receptor of activated protein C kinase 1
NCBI Official Synonym Full Names
receptor for activated C kinase 1
NCBI Official Symbol
RACK1
NCBI Official Synonym Symbols
H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
UniProt Protein Name
Receptor of activated protein C kinase 1
UniProt Synonym Protein Names
Cell proliferation-inducing gene 21 protein; Guanine nucleotide-binding protein subunit beta-2-like 1; Guanine nucleotide-binding protein subunit beta-like protein 12.3; Human lung cancer oncogene 7 protein; HLC-7
Protein Family
Receptor of activated protein C kinase
UniProt Gene Name
RACK1
UniProt Synonym Gene Names
HLC-7
UniProt Entry Name
RACK1_HUMAN
NCBI Protein Information
receptor of activated protein C kinase 1

UniProt Comments for RACK1

RACK1: a G-beta like protein containing 7 WD domains. A presumptive scaffold protein, linking protein kinase C to its substrates. Binds inositol 1,4,5-trisphosphate receptors and regulates Ca2+ release by enhancing inositol 1,4,5-trisphosphate receptor binding affinity for inositol 1,4,5-trisphosphate. An insulin-like growth factor 1 receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death.

Protein type: G protein, heterotrimeric; G protein; Adaptor/scaffold; Motility/polarity/chemotaxis; G protein, heterotrimeric beta; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cell soma; cytoplasm; cytosol; dendrite; midbody; mitochondrion; nucleus; perikaryon; perinuclear region of cytoplasm; phagocytic cup; small ribosomal subunit

Molecular Function: caspase activator activity; enzyme binding; ion channel inhibitor activity; protein binding; protein complex scaffold; protein homodimerization activity; protein kinase C binding; protein phosphatase binding; protein tyrosine kinase inhibitor activity; receptor binding; receptor tyrosine kinase binding; SH2 domain binding

Biological Process: caspase activation; cell cycle; gastrulation; negative regulation of cell growth; negative regulation of peptidyl-serine phosphorylation; negative regulation of phagocytosis; negative regulation of protein kinase B signaling cascade; negative regulation of translation; negative regulation of Wnt receptor signaling pathway; osteoblast differentiation; positive regulation of apoptosis; positive regulation of cAMP catabolic process; positive regulation of cell migration; positive regulation of cyclic-nucleotide phosphodiesterase activity; positive regulation of Golgi to plasma membrane protein transport; positive regulation of GTPase activity; positive regulation of mitochondrial depolarization; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of protein homooligomerization; regulation of cell cycle; regulation of cell division; regulation of protein localization; rhythmic process; viral reproduction
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Research Articles on RACK1

1. The results suggest a mode of interaction that allows spectrin to attach to the N-terminal/C-terminal part of RACK through the inter-helical AB and BC loops and adopt a multitude of configurations in between the two limiting configurations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RACK1 antibody

 Reference Product  PubMed Publications
 GNB2L1 antibody  >187 publications with RACK1 and GNB2L1
 RPS3A antibody  >1 publications with RACK1 and RPS3A
 RPS3 antibody  >1 publications with RACK1 and RPS3

Pathways associated with anti-RACK1 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Death Receptor Signalling Pathway antibodies  Death Receptor Signalling Pathway Diagram
 Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway antibodies  Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway Diagram
 IGF1 Pathway antibodies  IGF1 Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 Interferon Type I Pathway antibodies  Interferon Type I Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram

Diseases associated with anti-RACK1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >44 publications with RACK1 and Carcinoma
 Neoplasm Metastasis Antibodies  >24 publications with RACK1 and Neoplasm Metastasis
 Necrosis Antibodies  >20 publications with RACK1 and Necrosis
 Breast Neoplasms Antibodies  >16 publications with RACK1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >14 publications with RACK1 and Nervous System Diseases
 Inflammation Antibodies  >12 publications with RACK1 and Inflammation
 Liver Diseases Antibodies  >12 publications with RACK1 and Liver Diseases
 Carcinoma, Squamous Cell Antibodies  >12 publications with RACK1 and Carcinoma, Squamous Cell
 Fibrosis Antibodies  >10 publications with RACK1 and Fibrosis
 Prostatic Neoplasms Antibodies  >9 publications with RACK1 and Prostatic Neoplasms

Organs/Tissues associated with anti-RACK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >68 publications with RACK1 and Brain
 Blood Antibodies  >39 publications with RACK1 and Blood
 Muscle Antibodies  >28 publications with RACK1 and Muscle
 Vascular Antibodies  >24 publications with RACK1 and Vascular
 Liver Antibodies  >23 publications with RACK1 and Liver
 Heart Antibodies  >23 publications with RACK1 and Heart
 Kidney Antibodies  >21 publications with RACK1 and Kidney
 Lung Antibodies  >15 publications with RACK1 and Lung
 Nerve Antibodies  >13 publications with RACK1 and Nerve
 Prostate Antibodies  >13 publications with RACK1 and Prostate
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