Anti-n-MYC Polyclonal Antibody – Cat# MBS177938

Anti- n-Myc Picoband antibody, MBS177938, Western blotting
All lanes: Anti n-Myc (MBS177938) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: A431 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD

n-MYC Antibody – Polyclonal

Name: n-MYC Antibody; Polyclonal; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS177938
Price: 485 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177938

Description: Rabbit IgG polyclonal antibody for N-myc proto-oncogene protein(MYCN) detection. Tested with WB in Human;Rat.Background: N-myc proto-oncogene protein, also known as N-Myc or basic helix-loop-helix protein 37 (bHLHe37), is a protein that in humans… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

n-Myc, Polyclonal Antibody

Full Product Name

Anti-n-Myc Antibody

Product Gene Name

anti-n-Myc antibody

Product Synonym Names

N-myc proto-oncogene protein; bHLHe37; Class E basic helix-loop-helix protein 37; MODED; mycn; MYCN_HUMAN; N myc; N myc proto oncogene protein; N-myc proto-oncogene protein; Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene; Neuroblastoma MYC oncogene; NMYC; NMYC proto oncogene protein; ODED; Oncogene NMYC; pp65/67; v myc avian myelocytomatosis viral related oncogene neuroblastoma derived; v myc myelocytomatosis viral related oncogene neuroblastoma derived; v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog

Clonality

Polyclonal

Species Reactivity

Human, Rat

Form/Format

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen Affinity Purified

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

49,561 Da

OMIM

164280

3D Structure

ModBase 3D Structure for P04198

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human n-Myc (428-464aa EYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC), different from the related mouse and rat sequences by two amino acids.

Ig Type

Rabbit IgG

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-n-Myc antibody

Western Blot (WB)

Application Notes for anti-n-Myc antibody

Western Blot Concentration: 0.1-0.5ug/ml

Product References and Citations for anti-n-Myc antibody

1. Armstrong BC, Krystal GW (1992). "Isolation and characterization of complementary DNA for N-cym, a gene encoded by the DNA strand opposite to N-myc.". Cell Growth Differ. 3 (6): 385-90. 2. Cheng JM, Hiemstra JL, Schneider SS, Naumova A, Cheung NK, Cohn SL, Diller L, Sapienza C, Brodeur GM (June 1993). "Preferential amplification of the paternal allele of the N-myc gene in human neuroblastomas". Nat. Genet. 4 (2): 191-4. 3. Knoepfler PS, Cheng PF, Eisenman RN (2002). "N-myc is essential during neurogenesis for the rapid expansion of progenitor cell populations and the inhibition of neuronal differentiation.". Genes Dev. 16 (20): 2699-712.

Other Notes

Small volumes of anti-n-Myc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for n-Myc purchase

MBS177938 is an antibody targeting n-Myc. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for n-Myc. It may not necessarily be applicable to this product.

NCBI GI #

648216288

NCBI GeneID

4613

NCBI Accession #

NP_001280157.1

UniProt Primary Accession #

P04198

UniProt Secondary Accession #

Q53XS5; Q6LDT9

UniProt Related Accession #

P04198

GenBank Nucleotide #

NM_001293228.1

NCBI Official Full Name

N-myc proto-oncogene protein isoform 1

NCBI Official Synonym Full Names

v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog

NCBI Official Symbol

MYCN

NCBI Official Synonym Symbols

NMYC; ODED; MODED; N-myc; bHLHe37

UniProt Protein Name

N-myc proto-oncogene protein

UniProt Synonym Protein Names

Class E basic helix-loop-helix protein 37; bHLHe37

Protein Family

N-myc 2 proto-oncogene protein

UniProt Gene Name

MYCN

UniProt Synonym Gene Names

BHLHE37; NMYC; bHLHe37

UniProt Entry Name

MYCN_HUMAN

NCBI Protein Information

N-myc proto-oncogene protein

NCBI Summary for n-Myc

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

UniProt Comments for n-Myc

N-Myc: a proto-oncogenic transcription factor. Plays a role in cell proliferation, apoptosis and in the development of human tumors.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2p24.3

Cellular Component: chromatin; nucleus

Molecular Function: DNA binding; protein binding; protein dimerization activity; transcription factor activity

Biological Process: branching morphogenesis of a tube; cartilage condensation; embryonic digit morphogenesis; embryonic skeletal morphogenesis; lung development; negative regulation of astrocyte differentiation; positive regulation of mesenchymal cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of auditory receptor cell differentiation; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Disease: Feingold Syndrome 1; Tracheoesophageal Fistula With Or Without Esophageal Atresia

Research Articles on n-Myc

1. Dual-colored fluorescence in situ hybridization on 16 primary neuroblastomas, show that those with unbalanced gain of 17q have high MYCN expression, those with no gain have medium expression, and those with numerical gain have low expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-n-Myc antibody

Reference Product	PubMed Publications
MAX antibody	>632 publications with n-Myc and MAX
CCND1 antibody	>172 publications with n-Myc and CCND1
CDKN2A antibody	>147 publications with n-Myc and CDKN2A
SP1 antibody	>120 publications with n-Myc and SP1
PTEN antibody	>117 publications with n-Myc and PTEN
AURKA antibody	>43 publications with n-Myc and AURKA
SIRT1 antibody	>31 publications with n-Myc and SIRT1
TRRAP antibody	>31 publications with n-Myc and TRRAP
NDRG1 antibody	>15 publications with n-Myc and NDRG1
NTRK1 antibody	>14 publications with n-Myc and NTRK1

Pathways associated with anti-n-Myc antibody

Products by Pathway	Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-n-Myc antibody

Disease Name	Pubmed Publications
Neuroblastoma Antibodies	>1734 publications with n-Myc and Neuroblastoma
Cell Transformation, Neoplastic Antibodies	>1106 publications with n-Myc and Cell Transformation, Neoplastic
Chromosome Aberrations Antibodies	>743 publications with n-Myc and Chromosome Aberrations
Adenocarcinoma Antibodies	>699 publications with n-Myc and Adenocarcinoma
Breast Neoplasms Antibodies	>639 publications with n-Myc and Breast Neoplasms
Disease Models, Animal Antibodies	>613 publications with n-Myc and Disease Models, Animal
Neoplasms, Experimental Antibodies	>534 publications with n-Myc and Neoplasms, Experimental
Liver Neoplasms Antibodies	>430 publications with n-Myc and Liver Neoplasms
Liver Diseases Antibodies	>425 publications with n-Myc and Liver Diseases
Neoplasm Metastasis Antibodies	>384 publications with n-Myc and Neoplasm Metastasis

Organs/Tissues associated with anti-n-Myc antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>739 publications with n-Myc and Liver
Bone Antibodies	>591 publications with n-Myc and Bone
Lung Antibodies	>545 publications with n-Myc and Lung
Brain Antibodies	>532 publications with n-Myc and Brain
Muscle Antibodies	>438 publications with n-Myc and Muscle
Kidney Antibodies	>302 publications with n-Myc and Kidney
Embryonic Tissue Antibodies	>283 publications with n-Myc and Embryonic Tissue
Heart Antibodies	>192 publications with n-Myc and Heart
Eye Antibodies	>104 publications with n-Myc and Eye
Thymus Antibodies	>98 publications with n-Myc and Thymus