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TPA Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177973

Description: Rabbit IgG polyclonal antibody for Tissue-type plasminogen activator(PLAT) detection. Tested with WB in Human;Mouse.Background: PLAT is also known as tPA. This gene encodes tissue-type plasminogen activator, a secreted serine protease which… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TPA, Polyclonal Antibody
Full Product Name
Anti-TPA Antibody
Product Gene Name
Product Synonym Names
Tissue-type plasminogen activator; Alteplase; DKFZp686I03148; Plasminogen activator tissue; Plasminogen activator tissue type; Plasminogen Activator Tissue Type Isoform 1 Preproprotein; PLAT; Reteplase; t PA; T Plasminogen Activator; t-PA; T-plasminogen activator; Tissue plasminogen activator (t PA); Tissue type plasminogen activator; Tissue-type plasminogen activator chain B; TPA; TPA_HUMAN; TPA1; plasminogen activator, tissue
Clonality
Polyclonal
Species Reactivity
Human, Mouse
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
44,373 Da
OMIM
173370
3D Structure
ModBase 3D Structure for P00750
Immunogen
E Coli-derived human TPA recombinant protein (Position: H366-P562). Human TPA shares 83% and 84% amino acid (aa) sequence identity with mouse and rat TPA, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TPA antibody
Western Blot (WB)
Application Notes for anti-TPA antibody
Western Blot Concentration: 0.1-0.5ug/ml

Western Blot (WB) of anti-TPA antibody

Anti-TPA Picoband antibody, MBS177973, Western blotting
All lanes: Anti TPA (MBS177973) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: A375 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 85KD
Observed bind size: 85KD
Anti-TPA Polyclonal Antibody – Cat# MBS177973

Product References and Citations for anti-TPA antibody

1. Marsh EB, et al. Serum creatinine may indicate risk of symptomatic intracranial hemorrhage after intravenous tissue plasminogen activator (IV tPA). Medicine (Baltimore), 2013 Nov. 2. Valle-Garay E, et al. tPA Alu (I/D) polymorphism associates with bacterial osteomyelitis. J Infect Dis, 2013 Jul 15.

Other Notes

Small volumes of anti-TPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPA purchase

MBS177973 is an antibody targeting TPA. Reactive in Human, Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TPA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tissue-type plasminogen activator isoform 1 preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue type
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Synonym Protein Names
INN: Alteplase; INN: Reteplase
UniProt Gene Name
PLAT
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
UniProt Entry Name
TPA_HUMAN
NCBI Protein Information
tissue-type plasminogen activator

NCBI Summary for TPA

This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
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UniProt Comments for TPA

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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