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Occludin (OCLN) Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177977

Description: Rabbit IgG polyclonal antibody for Occludin(OCLN) detection. Tested with WB, IHC-P in Human.Background: Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly… More details

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Product Overview

Online Datasheet in PDF
Product Name
Occludin (OCLN), Polyclonal Antibody
Full Product Name
Anti-Occludin Antibody
Product Gene Name
anti-OCLN antibody
Product Synonym Names
Occludin; BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin; occludin
Clonality
Polyclonal
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
8,175 Da
OMIM
251290
3D Structure
ModBase 3D Structure for Q16625
Immunogen
E Coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-OCLN antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-OCLN antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-OCLN antibody

Anti- Occludin antibody, Western blotting
All lanes: Anti Occludin at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Anti-OCLN Polyclonal Antibody – Cat# MBS177977

Immunohistochemistry (IHC) of anti-OCLN antibody

Anti- Occludin antibody,IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-OCLN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177977

Product References and Citations for anti-OCLN antibody

1. Ando-Akatsuka, Y., Saitou, M., Hirase, T., Kishi, M., Sakakibara, A., Itoh, M., Yonemura, S., Furuse, M., Tsukita, S. Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. J. Cell Biol. 133: 43-47, 1996. 2. Barrios-Rodiles, M., Brown, K. R., Ozdamar, B., Bose, R., Liu, Z., Donovan, R. S., Shinjo, F., Liu, Y., Dembowy, J., Taylor, I. W., Luga, V., Przulj, N., Robinson, M., Suzuki, H., Hayashizaki, Y., Jurisica, I., Wrana, J. L. High-throughput mapping of a dynamic signaling network in mammalian cells. Science 307: 1621-1625, 2005. 3. Du, D., Xu, F., Yu, L., Zhang, C., Lu, X., Yuan, H., Huang, Q., Zhang, F., Bao, H., Jia, L., Wu, X., Zhu, X., Zhang, X., Zhang, Z., Chen, Z. The tight junction protein, occludin, regulates the directional migration of epithelial cells. Dev. Cell 18: 52-63, 2010.

Other Notes

Small volumes of anti-OCLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OCLN purchase

MBS177977 is an antibody targeting OCLN. Also known as Anti-Occludin Antibody. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.

NCBI GI #
327478414
NCBI GeneID
100506658
NCBI Accession #
NP_001192183.1
UniProt Primary Accession #
Q16625
UniProt Secondary Accession #
Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9
UniProt Related Accession #
Q16625
GenBank Nucleotide #
NM_001205254.1
NCBI Official Full Name
occludin isoform a
NCBI Official Synonym Full Names
occludin
NCBI Official Symbol
OCLN
NCBI Official Synonym Symbols
BLCPMG; PPP1R115
UniProt Protein Name
Occludin
Protein Family
Occludin
UniProt Gene Name
OCLN
UniProt Entry Name
OCLN_HUMAN
NCBI Protein Information
occludin

NCBI Summary for OCLN

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
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UniProt Comments for OCLN

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: apical plasma membrane; apicolateral plasma membrane; cell junction; cytoplasmic vesicle; cytosol; endocytic vesicle; integral to membrane; intercellular junction; plasma membrane; tight junction

Molecular Function: protein binding; protein domain specific binding; thiopurine S-methyltransferase activity

Biological Process: intercellular junction assembly and maintenance; methylation; protein complex assembly; S-adenosylhomocysteine metabolic process; S-adenosylmethionine metabolic process

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
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Research Articles on OCLN

1. Angiopoietin-1 Regulates Brain Endothelial Permeability through PTPN-2 Mediated Tyrosine Dephosphorylation of Occludin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OCLN antibody

 Reference Product  PubMed Publications
 TJP1 antibody  >553 publications with OCLN and TJP1
 TJP2 antibody  >49 publications with OCLN and TJP2
 TJP3 antibody  >14 publications with OCLN and TJP3
 CGN antibody  >7 publications with OCLN and CGN
 TGFBR1 antibody  >2 publications with OCLN and TGFBR1
 YES1 antibody  >1 publications with OCLN and YES1

Pathways associated with anti-OCLN antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cell Adhesion Proteins Pathway antibodies  Apoptotic Cleavage Of Cell Adhesion Proteins Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-OCLN antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >159 publications with OCLN and Inflammation
 Disease Models, Animal Antibodies  >133 publications with OCLN and Disease Models, Animal
 Necrosis Antibodies  >93 publications with OCLN and Necrosis
 Cardiovascular Diseases Antibodies  >84 publications with OCLN and Cardiovascular Diseases
 Edema Antibodies  >66 publications with OCLN and Edema
 Liver Diseases Antibodies  >53 publications with OCLN and Liver Diseases
 Retinal Diseases Antibodies  >34 publications with OCLN and Retinal Diseases
 Lung Diseases Antibodies  >26 publications with OCLN and Lung Diseases
 Kidney Diseases Antibodies  >17 publications with OCLN and Kidney Diseases
 Hemorrhage Antibodies  >16 publications with OCLN and Hemorrhage

Organs/Tissues associated with anti-OCLN antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >288 publications with OCLN and Brain
 Intestine Antibodies  >258 publications with OCLN and Intestine
 Liver Antibodies  >134 publications with OCLN and Liver
 Eye Antibodies  >107 publications with OCLN and Eye
 Kidney Antibodies  >75 publications with OCLN and Kidney
 Testis Antibodies  >59 publications with OCLN and Testis
 Embryonic Tissue Antibodies  >54 publications with OCLN and Embryonic Tissue
 Lung Antibodies  >53 publications with OCLN and Lung
 Stomach Antibodies  >22 publications with OCLN and Stomach
 Muscle Antibodies  >16 publications with OCLN and Muscle
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