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Surfactant Protein D (SFTPD) Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178061

Description: Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein D(SFTPD) detection. Tested with WB, IHC-P, ELISA in Human; Rat. Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Surfactant protein D (SFTPD), Polyclonal Antibody
Full Product Name
Anti-Surfactant protein D Antibody
Product Gene Name
anti-SFTPD antibody
Product Synonym Names
Pulmonary surfactant-associated protein D; COLEC 7; COLEC7; Collectin-7; Collectin7; Lung surfactant protein D; PSP D; PSP-D; PSP-D Surfactant protein D; PSPD; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein D; Pulmonary surfactant associated protein PSP-D; Pulmonary surfactant-associated protein D; SFTP 4; SFTP4; SFTPD; SFTPD_HUMAN; SP D; SP-D; Surfactant associated protein pulmonary 4; Surfactant pulmonary associated protein D; surfactant protein D
Clonality
Polyclonal
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
37,728 Da
OMIM
178635
3D Structure
ModBase 3D Structure for P35247
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Surfactant protein D (292-321aa RSAAENAALQQLVVAKNEAAFLSMTD SKTE), different from the related mouse and rat sequences by eight amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SFTPD antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-SFTPD antibody
ELISA Concentration: 0.1-0.5ug/ml
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-SFTPD antibody

Anti- Surfactant protein D Picoband antibody, MBS178061, Western blotting
All lanes: Anti Surfactant protein D (MBS178061) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: PANC Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-SFTPD Polyclonal Antibody – Cat# MBS178061

Immunohistochemistry (IHC) of anti-SFTPD antibody

Anti- Surfactant protein D Picoband antibody, MBS178061, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-SFTPD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178061

Immunohistochemistry (IHC) of anti-SFTPD antibody

Figure 3. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (MBS178061).
Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Surfactant protein D Antibody (MBS178061) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-SFTPD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178061

Immunohistochemistry (IHC) of anti-SFTPD antibody

Figure 4. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (MBS178061).
Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Surfactant protein D Antibody (MBS178061) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-SFTPD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178061

Immunohistochemistry (IHC) of anti-SFTPD antibody

Figure 5. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (MBS178061).
Surfactant protein D was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Surfactant protein D Antibody (MBS178061) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5
Anti-SFTPD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178061

Product References and Citations for anti-SFTPD antibody

1. Brandt EB, Mingler MK, Stevenson MD, Wang N, Khurana Hershey GK, Whitsett JA, Rothenberg ME (May 2008). "Surfactant protein D alters allergic lung responses in mice and human subjects". J. Allergy Clin. Immunol. 121 (5): 1140-1147.e2. 2. Lu J, Willis AC, Reid KB (June 1992)."Purification, characterization and cDNA cloning of human lung surfactant protein D". Biochem. J. 284. 284 (Pt 3): 795-802. 3. Rust K, Grosso L, Zhang V, Chang D, Persson A, Longmore W, Cai GZ, Crouch E (October 1991). "Human surfactant protein D: SP-D contains a C-type lectin carbohydrate recognition domain". Arch. Biochem. Biophys. 290 (1): 116-26.

Other Notes

Small volumes of anti-SFTPD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SFTPD purchase

MBS178061 is an antibody targeting SFTPD. Also known as Anti-Surfactant protein D Antibody. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for SFTPD. It may not necessarily be applicable to this product.

NCBI GI #
61699226
NCBI GeneID
6441
NCBI Accession #
NP_003010.4
UniProt Primary Accession #
P35247
UniProt Secondary Accession #
Q5T0M3; Q6FH08; Q86YK9; Q8TCD8; Q9UCJ2; Q9UCJ3
UniProt Related Accession #
P35247
GenBank Nucleotide #
NM_003019.4
NCBI Official Full Name
pulmonary surfactant-associated protein D
NCBI Official Synonym Full Names
surfactant protein D
NCBI Official Symbol
SFTPD
NCBI Official Synonym Symbols
SP-D; PSP-D; SFTP4; COLEC7
UniProt Protein Name
Pulmonary surfactant-associated protein D
UniProt Synonym Protein Names
Collectin-7; Lung surfactant protein D
Protein Family
Pulmonary surfactant-associated protein
UniProt Gene Name
SFTPD
UniProt Synonym Gene Names
COLEC7; PSPD; SFTP4; PSP-D; SP-D
UniProt Entry Name
SFTPD_HUMAN
NCBI Protein Information
pulmonary surfactant-associated protein D

NCBI Summary for SFTPD

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

UniProt Comments for SFTPD

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q22.2-q23.1

Cellular Component: clathrin-coated endocytic vesicle; collagen; endocytic vesicle; endoplasmic reticulum membrane; extracellular region; extracellular space; lamellar body; lysosome; proteinaceous extracellular matrix

Molecular Function: carbohydrate binding; protein binding

Biological Process: alveolus development; cellular protein metabolic process; defense response to bacterium; innate immune response; macrophage chemotaxis; negative regulation of interleukin-2 biosynthetic process; negative regulation of T cell proliferation; positive regulation of phagocytosis; receptor-mediated endocytosis; regulation of cytokine production; regulation of immune response; respiratory gaseous exchange; surfactant homeostasis
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Research Articles on SFTPD

1. The SP-D level showed positive correlations with carotid IMT and coronary artery calcification in patients on long-term hemodialysis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SFTPD antibody

 Products by Pathway  Pathway Diagram
 ABC Transporter Disorders Pathway antibodies  ABC Transporter Disorders Pathway Diagram
 Defective ABCA3 Causes Pulmonary Surfactant Metabolism Dysfunction Type 3 (SMDP3) Pathway antibodies  Defective ABCA3 Causes Pulmonary Surfactant Metabolism Dysfunction Type 3 (SMDP3) Pathway Diagram
 Defective CSF2RA Causes Pulmonary Surfactant Metabolism Dysfunction 4 (SMDP4) Pathway antibodies  Defective CSF2RA Causes Pulmonary Surfactant Metabolism Dysfunction 4 (SMDP4) Pathway Diagram
 Defective CSF2RB Causes Pulmonary Surfactant Metabolism Dysfunction 5 (SMDP5) Pathway antibodies  Defective CSF2RB Causes Pulmonary Surfactant Metabolism Dysfunction 5 (SMDP5) Pathway Diagram
 Defective SFTPA2 Causes Idiopathic Pulmonary Fibrosis (IPF) Pathway antibodies  Defective SFTPA2 Causes Idiopathic Pulmonary Fibrosis (IPF) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Surfactant Metabolism Pathway antibodies  Diseases Associated With Surfactant Metabolism Pathway Diagram
 Diseases Of Metabolism Pathway antibodies  Diseases Of Metabolism Pathway Diagram
 Disorders Of Transmembrane Transporters Pathway antibodies  Disorders Of Transmembrane Transporters Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram

Diseases associated with anti-SFTPD antibody

 Disease Name  Pubmed Publications
 Respiratory Tract Diseases Antibodies  >32 publications with SFTPD and Respiratory Tract Diseases
 Lung Diseases Antibodies  >29 publications with SFTPD and Lung Diseases
 Inflammation Antibodies  >21 publications with SFTPD and Inflammation
 Lung Injury Antibodies  >9 publications with SFTPD and Lung Injury
 Pneumonia Antibodies  >5 publications with SFTPD and Pneumonia
 Respiratory Hypersensitivity Antibodies  >5 publications with SFTPD and Respiratory Hypersensitivity
 Respiration Disorders Antibodies  >4 publications with SFTPD and Respiration Disorders
 Lung Neoplasms Antibodies  >4 publications with SFTPD and Lung Neoplasms
 Premature Birth Antibodies  >3 publications with SFTPD and Premature Birth
 Necrosis Antibodies  >3 publications with SFTPD and Necrosis

Organs/Tissues associated with anti-SFTPD antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >39 publications with SFTPD and Lung
 Connective Tissue Antibodies  >2 publications with SFTPD and Connective Tissue
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