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Nestin (NES) Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178078

Description: Rabbit IgG polyclonal antibody for Protein Mdm4(MDM4) detection. Tested with WB, IHC-P in Human;Rat.Background: Nestin is a type VI intermediate filament (IF) protein that in humans is encoded by the NES gene. This gene is mapped to 1q23.1.… More details

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Product Overview

Online Datasheet in PDF
Product Name
Nestin, Polyclonal Antibody
Full Product Name
Anti-Nestin Antibody
Product Gene Name
anti-NES antibody
Product Synonym Names
Nestin; ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin; nestin
Clonality
Polyclonal
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
177,439 Da
OMIM
600915
3D Structure
ModBase 3D Structure for P48681
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin (252-290aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NES antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-NES antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-NES antibody

Western blot analysis of Nestin expression in HELA whole cell lysates (lane 1) and HEPG2 whole cell lysates (lane 2). Nestin at 230KD was detected using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Anti-NES Polyclonal Antibody – Cat# MBS178078

Immunohistochemistry (IHC) of anti-NES antibody

Nestin was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Anti-NES Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178078

Immunohistochemistry (IHC) of anti-NES antibody

Nestin was detected in paraffin-embedded sections of human melanoma tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Anti-NES Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178078

Immunohistochemistry (IHC) of anti-NES antibody

Figure 4. IHC analysis of Nestin using anti-Nestin antibody (MBS178078).
Nestin was detected in frozen section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Nestin Antibody (MBS178078) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-NES Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178078

Immunohistochemistry (IHC) of anti-NES antibody

Figure 5. IHC analysis of Nestin using anti-Nestin antibody (MBS178078).
Nestin was detected in frozen section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Nestin Antibody (MBS178078) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-NES Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178078

Product References and Citations for anti-NES antibody

1. Guérette D, Khan PA, Savard PE, Vincent M (2007). "Molecular evolution of type VI intermediate filament proteins". BMC Evol. Biol. 7: 164. 2. Michalczyk K, Ziman M (April 2005). "Nestin structure and predicted function in cellular cytoskeletal organisation". Histol. Histopathol. 20 (2): 665-71.

Other Notes

Small volumes of anti-NES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NES purchase

MBS178078 is an antibody targeting NES. Also known as Anti-Nestin Antibody. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for NES. It may not necessarily be applicable to this product.

NCBI GI #
38176300
NCBI GeneID
10763
NCBI Accession #
NP_006608.1
UniProt Primary Accession #
P48681
UniProt Secondary Accession #
O00552; Q3LIF5; Q5SYZ6
UniProt Related Accession #
P48681
GenBank Nucleotide #
NM_006617.1
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
Nbla00170
UniProt Protein Name
Nestin
Protein Family
Nestin
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN
NCBI Protein Information
nestin

NCBI Summary for NES

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

UniProt Comments for NES

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cytoplasm; intermediate filament; intermediate filament cytoskeleton

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: brain development; cell projection morphogenesis; central nervous system development; embryonic camera-type eye development; G2/M transition of mitotic cell cycle; negative regulation of catalytic activity; negative regulation of neuron apoptosis; negative regulation of protein binding; positive regulation of intermediate filament depolymerization
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Research Articles on NES

1. Nestin is expressed in acute leukemia and might be a useful immunohistochemical marker for identifying acute myeloid leukemia and acute lymphoblastic leukemia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NES antibody

 Reference Product  PubMed Publications
 FGF2 antibody  >110 publications with NES and FGF2
 DCX antibody  >25 publications with NES and DCX
 STAT3 antibody  >21 publications with NES and STAT3
 BMP4 antibody  >17 publications with NES and BMP4
 MYCN antibody  >16 publications with NES and MYCN
 CDK5 antibody  >14 publications with NES and CDK5
 VSX2 antibody  >6 publications with NES and VSX2
 CDK1 antibody  >6 publications with NES and CDK1
 POU3F2 antibody  >4 publications with NES and POU3F2
 RBFOX3 antibody  >1 publications with NES and RBFOX3

Diseases associated with anti-NES antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1000 publications with NES and Nervous System Diseases
 Brain Diseases Antibodies  >695 publications with NES and Brain Diseases
 Disease Models, Animal Antibodies  >271 publications with NES and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >234 publications with NES and Cardiovascular Diseases
 Seizures Antibodies  >163 publications with NES and Seizures
 Breast Neoplasms Antibodies  >77 publications with NES and Breast Neoplasms
 Nerve Degeneration Antibodies  >76 publications with NES and Nerve Degeneration
 Heart Diseases Antibodies  >71 publications with NES and Heart Diseases
 Necrosis Antibodies  >61 publications with NES and Necrosis
 Inflammation Antibodies  >59 publications with NES and Inflammation

Organs/Tissues associated with anti-NES antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >2107 publications with NES and Nerve
 Brain Antibodies  >1362 publications with NES and Brain
 Embryonic Tissue Antibodies  >709 publications with NES and Embryonic Tissue
 Vascular Antibodies  >269 publications with NES and Vascular
 Muscle Antibodies  >265 publications with NES and Muscle
 Eye Antibodies  >185 publications with NES and Eye
 Skin Antibodies  >160 publications with NES and Skin
 Heart Antibodies  >118 publications with NES and Heart
 Pancreas Antibodies  >116 publications with NES and Pancreas
 Kidney Antibodies  >101 publications with NES and Kidney
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