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HINT1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178092

Description: Rabbit IgG polyclonal antibody for Histidine triad nucleotide-binding protein 1(HINT1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.Background: Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the… More details

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Product Overview

Online Datasheet in PDF
Product Name
HINT1, Polyclonal Antibody
Full Product Name
Anti-HINT1 Antibody
Product Gene Name
anti-HINT1 antibody
Product Synonym Names
Histidine triad nucleotide-binding protein 1; HINT 1; HINT1; HINT1_HUMAN; Histidine triad nucleotide binding protein 1; Histidine triad nucleotide-binding protein 1; PKCI 1; PKCI-1; PKCI1; PRKCNH1; Protein kinase C inhibitor 1; Protein kinase C interacting protein 1; Protein kinase C-interacting protein 1; histidine triad nucleotide binding protein 1
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
13,802 Da
OMIM
137200
3D Structure
ModBase 3D Structure for P49773
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HINT1 (59-92aa HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK), different from the related mouse and rat sequences by three amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HINT1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-HINT1 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-HINT1 antibody

Anti- HINT1 Picoband antibody, MBS178092, Western blotting
All lanes: Anti HINT1 (MBS178092) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 14KD
Observed bind size: 14KD
Anti-HINT1 Polyclonal Antibody – Cat# MBS178092

Immunohistochemistry (IHC) of anti-HINT1 antibody

Anti- HINT1 Picoband antibody, MBS178092, IHC(P)
IHC(P): Mouse Intestine Tissue
Anti-HINT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178092

Immunohistochemistry (IHC) of anti-HINT1 antibody

Anti- HINT1 Picoband antibody, MBS178092, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-HINT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178092

Immunohistochemistry (IHC) of anti-HINT1 antibody

Anti- HINT1 Picoband antibody, MBS178092, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-HINT1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178092

Product References and Citations for anti-HINT1 antibody

1. "Entrez Gene: HINT1 histidine triad nucleotide binding protein 1". 2. Brzoska PM, Chen H, Levin NA, Kuo WL, Collins C, Fu KK, Gray JW, Christman MF (Feb 1997). "Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)". Genomics 36(1): 151-6. 3. Wang L, Li H, Zhang Y, Santella RM, Weinstein IB (Apr 2009). "HINT1 inhibits beta-catenin/TCF4, USF2 and NFkappaB activity in human hepatoma cells". Int J Cancer 124 (7): 1526-34.

Other Notes

Small volumes of anti-HINT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HINT1 purchase

MBS178092 is an antibody targeting HINT1. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for HINT1. It may not necessarily be applicable to this product.

NCBI GI #
4885413
NCBI GeneID
3094
NCBI Accession #
NP_005331.1
UniProt Primary Accession #
P49773
UniProt Secondary Accession #
Q9H5W8
UniProt Related Accession #
P49773
GenBank Nucleotide #
NM_005340.6
NCBI Official Full Name
histidine triad nucleotide-binding protein 1
NCBI Official Synonym Full Names
histidine triad nucleotide binding protein 1
NCBI Official Symbol
HINT1
NCBI Official Synonym Symbols
HINT; NMAN; PKCI-1; PRKCNH1
UniProt Protein Name
Histidine triad nucleotide-binding protein 1
UniProt Synonym Protein Names
Adenosine 5'-monophosphoramidase; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; PKCI-1
Protein Family
Adenylylsulfatase
UniProt Gene Name
HINT1
UniProt Synonym Gene Names
HINT; PKCI1; PRKCNH1; PKCI-1
UniProt Entry Name
HINT1_HUMAN
NCBI Protein Information
histidine triad nucleotide-binding protein 1

NCBI Summary for HINT1

This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed. [provided by RefSeq, Dec 2015]
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UniProt Comments for HINT1

HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.

Protein type: Hydrolase; Cytoskeletal; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoplasm; cytoskeleton; histone deacetylase complex; nucleus; plasma membrane

Molecular Function: hydrolase activity; nucleotide binding; protein kinase C binding

Biological Process: positive regulation of calcium-mediated signaling; purine ribonucleotide catabolic process; regulation of transcription, DNA-dependent; signal transduction; transcription, DNA-dependent

Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive
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Research Articles on HINT1

1. HINT1 mutations seem to be one of the most frequent causes of inherited neuropathy and are probably the most frequent cause of HMN in Czech patients. We suggest all HMN/CMT2 patients be tested for the presence of the prevalent mutation, the p.R37P

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HINT1 antibody

 Reference Product  PubMed Publications
 MITF antibody  >8 publications with HINT1 and MITF
 KAT5 antibody  >1 publications with HINT1 and KAT5
 HINT3 antibody  >1 publications with HINT1 and HINT3

Diseases associated with anti-HINT1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >18 publications with HINT1 and Nervous System Diseases
 Schizophrenia Antibodies  >16 publications with HINT1 and Schizophrenia
 Pain Antibodies  >9 publications with HINT1 and Pain
 Brain Diseases Antibodies  >5 publications with HINT1 and Brain Diseases
 Liver Neoplasms Antibodies  >5 publications with HINT1 and Liver Neoplasms
 Gastrointestinal Diseases Antibodies  >4 publications with HINT1 and Gastrointestinal Diseases
 Cardiovascular Diseases Antibodies  >3 publications with HINT1 and Cardiovascular Diseases
 Learning Disorders Antibodies  >3 publications with HINT1 and Learning Disorders
 Inflammation Antibodies  >3 publications with HINT1 and Inflammation
 Inherited Peripheral Neuropathy Antibodies  >3 publications with HINT1 and Inherited Peripheral Neuropathy

Organs/Tissues associated with anti-HINT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >30 publications with HINT1 and Brain
 Liver Antibodies  >11 publications with HINT1 and Liver
 Blood Antibodies  >8 publications with HINT1 and Blood
 Muscle Antibodies  >6 publications with HINT1 and Muscle
 Embryonic Tissue Antibodies  >3 publications with HINT1 and Embryonic Tissue
 Bone Antibodies  >3 publications with HINT1 and Bone
 Kidney Antibodies  >3 publications with HINT1 and Kidney
 Skin Antibodies  >3 publications with HINT1 and Skin
 Eye Antibodies  >2 publications with HINT1 and Eye
 Lung Antibodies  >2 publications with HINT1 and Lung
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