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Cofilin (CFL1) Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178124

Description: Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
Cofilin (CFL1), Polyclonal Antibody
Full Product Name
Anti-Cofilin Antibody
Product Gene Name
anti-CFL1 antibody
Product Synonym Names
Cofilin-1; 18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18; cofilin 1 (non-muscle)
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
18,502 Da
OMIM
601442
3D Structure
ModBase 3D Structure for P23528
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin (122-152aa DAIKKKLTGIKHELQANCYEEVKDRCTLAEK), identical to the related mouse and rat sequences.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CFL1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CFL1 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-CFL1 antibody

Anti- Cofilin Picoband antibody, MBS178124, Western blotting
All lanes: Anti Cofilin (MBS178124) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Mouse Liver Tissue Lysate at 50ug
Lane 5: Mouse Brain Tissue Lysate at 50ug
Lane 6: Mouse Lung Tissue Lysate at 50ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: A549 Whole Cell Lysate at 40ug
Lane 10: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 19KD
Observed bind size: 19KD
Anti-CFL1 Polyclonal Antibody – Cat# MBS178124

Immunohistochemistry (IHC) of anti-CFL1 antibody

Anti- Cofilin Picoband antibody, MBS178124, IHC(P)
IHC(P): Mouse Intestine Tissue
Anti-CFL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178124

Immunohistochemistry (IHC) of anti-CFL1 antibody

Anti- Cofilin Picoband antibody, MBS178124, IHC(P)
IHC(P): Rat Intestine Tissue
Anti-CFL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178124

Immunohistochemistry (IHC) of anti-CFL1 antibody

Anti- Cofilin Picoband antibody, MBS178124, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-CFL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178124

Product References and Citations for anti-CFL1 antibody

1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000). "Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents". Cell Motil. Cytoskeleton 45 (4): 293-306. 3. Lappalainen P, Drubin DG (July 1997). "Cofilin promotes rapid actin filament turnover in vivo".Nature 388 (6637): 78-82.

Other Notes

Small volumes of anti-CFL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CFL1 purchase

MBS178124 is an antibody targeting CFL1. Also known as Anti-Cofilin Antibody. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CFL1. It may not necessarily be applicable to this product.

NCBI GI #
5031635
NCBI GeneID
1072
NCBI Accession #
NP_005498.1
UniProt Primary Accession #
P23528
UniProt Secondary Accession #
Q53Y87; Q9UCA2; B3KUQ1
UniProt Related Accession #
P23528
GenBank Nucleotide #
NM_005507.2
NCBI Official Full Name
cofilin-1
NCBI Official Synonym Full Names
cofilin 1
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; cofilin; HEL-S-15
UniProt Protein Name
Cofilin-1
UniProt Synonym Protein Names
18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform
Protein Family
Cofilin
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL; p18
UniProt Entry Name
COF1_HUMAN
NCBI Protein Information
cofilin-1

NCBI Summary for CFL1

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
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UniProt Comments for CFL1

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; membrane; nuclear matrix; nucleus; vesicle

Molecular Function: actin binding; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; cytoskeleton organization and biogenesis; negative regulation of apoptosis; regulation of cell morphogenesis; response to virus; Rho protein signal transduction
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Research Articles on CFL1

1. Cor1B, Cof1 and AIP1 work in concert through a temporally ordered pathway to induce highly efficient severing and disassembly of actin filaments.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CFL1 antibody

 Reference Product  PubMed Publications
 SSH1 antibody  >7 publications with CFL1 and SSH1
 PFN1 antibody  >5 publications with CFL1 and PFN1
 LIMK2 antibody  >4 publications with CFL1 and LIMK2
 WDR1 antibody  >2 publications with CFL1 and WDR1
 ACTB antibody  >2 publications with CFL1 and ACTB

Pathways associated with anti-CFL1 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHB-mediated Forward Signaling Pathway antibodies  EPHB-mediated Forward Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram

Diseases associated with anti-CFL1 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >15 publications with CFL1 and Death
 Nervous System Diseases Antibodies  >12 publications with CFL1 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >12 publications with CFL1 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >10 publications with CFL1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >10 publications with CFL1 and Breast Neoplasms
 Inflammation Antibodies  >8 publications with CFL1 and Inflammation
 Neoplasm Invasiveness Antibodies  >6 publications with CFL1 and Neoplasm Invasiveness
 Neoplasms, Experimental Antibodies  >4 publications with CFL1 and Neoplasms, Experimental
 Memory Disorders Antibodies  >3 publications with CFL1 and Memory Disorders
 Prenatal Exposure Delayed Effects Antibodies  >2 publications with CFL1 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with anti-CFL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >24 publications with CFL1 and Brain
 Muscle Antibodies  >17 publications with CFL1 and Muscle
 Blood Antibodies  >15 publications with CFL1 and Blood
 Nerve Antibodies  >14 publications with CFL1 and Nerve
 Lung Antibodies  >10 publications with CFL1 and Lung
 Kidney Antibodies  >6 publications with CFL1 and Kidney
 Uterus Antibodies  >5 publications with CFL1 and Uterus
 Vascular Antibodies  >5 publications with CFL1 and Vascular
 Bone Antibodies  >5 publications with CFL1 and Bone
 Embryonic Tissue Antibodies  >4 publications with CFL1 and Embryonic Tissue
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