Anti-MAFA Polyclonal Antibody – Cat# MBS178170

Anti- MAFA antibody, Western blotting
All lanes: Anti MAFA at 0.5ug/ml
WB: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 37KD
Observed bind size: 37KD

MAFA Antibody – Polyclonal

Name: MAFA Antibody; Polyclonal; Rat/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS178170
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178170

Description: Rabbit IgG polyclonal antibody for Transcription factor MafA(MAFA) detection. Tested with WB in Human;Rat.Background: MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MAFA, Polyclonal Antibody

Full Product Name

Anti-MAFA Antibody

Product Gene Name

anti-MAFA antibody

Product Synonym Names

Transcription factor MafA; HMafA; Mafa; MAFA_HUMAN; Pancreatic beta cell specific transcriptional activator; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1; Transcription factor MafA; Transcription factor RIPE3b1; v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian); v maf musculoaponeurotic fibrosarcoma oncogene homolog A; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A

Clonality

Polyclonal

Species Reactivity

Rat
Predicted to work with: Human

Form/Format

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen Affinity Purified

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

36,982 Da

OMIM

610303

3D Structure

ModBase 3D Structure for Q8NHW3

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human MAFA(136-167aa EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR), identical to the related mouse sequence.

Ig Type

Rabbit IgG

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MAFA antibody

Western Blot (WB)

Application Notes for anti-MAFA antibody

Western Blot Concentration: 0.1-0.5ug/ml

Product References and Citations for anti-MAFA antibody

1. Olbrot, M., Rud, J., Moss, L. G., Sharma, A. Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA. Proc. Nat. Acad. Sci. 99: 6737-6742, 2002. 2. Zhang, C., Moriguchi, T., Kajihara, M., Esaki, R., Harada, A., Shimohata, H., Oishi, H., Hamada, M., Morito, N., Hasegawa, K., Kudo, T., Engel, J. D., Yamamoto, M., Takahashi, S. MafA is a key regulator of glucose-stimulated insulin secretion. Molec. Cell. Biol. 25: 4969-4976, 2005.

Other Notes

Small volumes of anti-MAFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAFA purchase

MBS178170 is an antibody targeting MAFA. Reactive in Rat
Predicted to work with: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for MAFA. It may not necessarily be applicable to this product.

NCBI GI #

71274111

NCBI GeneID

389692

NCBI Accession #

NP_963883.2

UniProt Primary Accession #

Q8NHW3

UniProt Related Accession #

Q8NHW3

GenBank Nucleotide #

NM_201589.3

NCBI Official Full Name

transcription factor MafA

NCBI Official Synonym Full Names

MAF bZIP transcription factor A

NCBI Official Symbol

MAFA

NCBI Official Synonym Symbols

hMafA; RIPE3b1

UniProt Protein Name

Transcription factor MafA

UniProt Synonym Protein Names

Pancreatic beta-cell-specific transcriptional activator; Transcription factor RIPE3b1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

Protein Family

Transcription factor

UniProt Gene Name

MAFA

UniProt Entry Name

MAFA_HUMAN

NCBI Protein Information

transcription factor MafA

NCBI Summary for MAFA

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]

UniProt Comments for MAFA

MAFA: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1. Up-regulated by glucose. Belongs to the bZIP family. Maf subfamily.

Protein type: Transcription factor; DNA-binding; Tumor suppressor; Oncoprotein

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleus

Molecular Function: DNA binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity

Biological Process: insulin secretion; nitric oxide mediated signal transduction; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to glucose stimulus; transcription from RNA polymerase II promoter

Research Articles on MAFA

1. These findings demonstrate that regulation of monoamine levels by Mao activity in beta cells is pivotal for physiological insulin secretion and that loss of MaoB expression may contribute to the beta cell dysfunction in type 2 diabetes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAFA antibody

Reference Product	PubMed Publications
PDX1 antibody	>130 publications with MAFA and PDX1
NKX6-1 antibody	>41 publications with MAFA and NKX6-1
NEUROD1 antibody	>41 publications with MAFA and NEUROD1
INS antibody	>32 publications with MAFA and INS
PAX6 antibody	>24 publications with MAFA and PAX6
NKX2-2 antibody	>23 publications with MAFA and NKX2-2
FOXO1 antibody	>18 publications with MAFA and FOXO1
FOXA2 antibody	>16 publications with MAFA and FOXA2
GCK antibody	>7 publications with MAFA and GCK
IAPP antibody	>3 publications with MAFA and IAPP

Pathways associated with anti-MAFA antibody

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Maturity Onset Diabetes Of The Young Pathway antibodies	Maturity Onset Diabetes Of The Young Pathway Diagram
Maturity Onset Diabetes Of The Young Pathway antibodies	Maturity Onset Diabetes Of The Young Pathway Diagram
Regulation Of Beta-cell Development Pathway antibodies	Regulation Of Beta-cell Development Pathway Diagram
Regulation Of Gene Expression In Beta Cells Pathway antibodies	Regulation Of Gene Expression In Beta Cells Pathway Diagram
Type II Diabetes Mellitus Pathway antibodies	Type II Diabetes Mellitus Pathway Diagram
Type II Diabetes Mellitus Pathway antibodies	Type II Diabetes Mellitus Pathway Diagram
Type II Diabetes Mellitus Pathway antibodies	Type II Diabetes Mellitus Pathway Diagram

Diseases associated with anti-MAFA antibody

Disease Name	Pubmed Publications
Diabetes Mellitus Antibodies	>99 publications with MAFA and Diabetes Mellitus
Hyperglycemia Antibodies	>53 publications with MAFA and Hyperglycemia
Diabetes Mellitus, Type 2 Antibodies	>35 publications with MAFA and Diabetes Mellitus, Type 2
Pancreatic Diseases Antibodies	>25 publications with MAFA and Pancreatic Diseases
Insulin Resistance Antibodies	>18 publications with MAFA and Insulin Resistance
Hyperinsulinism Antibodies	>9 publications with MAFA and Hyperinsulinism
Inflammation Antibodies	>8 publications with MAFA and Inflammation
Necrosis Antibodies	>8 publications with MAFA and Necrosis
Glucose Intolerance Antibodies	>7 publications with MAFA and Glucose Intolerance
Weight Gain Antibodies	>5 publications with MAFA and Weight Gain

Organs/Tissues associated with anti-MAFA antibody

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>267 publications with MAFA and Pancreas
Embryonic Tissue Antibodies	>24 publications with MAFA and Embryonic Tissue
Kidney Antibodies	>15 publications with MAFA and Kidney