Anti-PSA Polyclonal Antibody – Cat# MBS178309

Anti- PSA Picoband antibody, MBS178309, Western blotting
All lanes: Anti PSA (MBS178309) at 0.5ug/ml
WB: Recombinant Human PSA Protein 0.5ng
Predicted bind size: 25KD
Observed bind size: 25KD

PSA Antibody – Polyclonal

Name: PSA Antibody; Polyclonal; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS178309
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178309

Description: Rabbit IgG polyclonal antibody for Prostate-specific antigen(KLK3) detection. Tested with WB in Human;Rat.Background: Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PSA, Polyclonal Antibody

Full Product Name

Anti-PSA Antibody

Product Gene Name

anti-PSA antibody

Product Synonym Names

Prostate-specific antigen; antigen, prostate-specific; APS; Gamma seminoprotein; Gamma-seminoprotein; hK3; Kallikrein 3; Kallikrein 3, (prostate specific antigen); Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; KLK3; KLK3_HUMAN; P 30 antigen; P-30 antigen; P30 antigen; Prostate Specific Antigen; Prostate-specific antigen; PSA; Semenogelase; Seminin; kallikrein-related peptidase 3

Clonality

Polyclonal

Species Reactivity

Human, Rat

Form/Format

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen Affinity Purified

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

OMIM

176820

3D Structure

ModBase 3D Structure for P07288

Immunogen

E Coli-derived human PSA recombinant protein (Position: I25-P261). Human PSA shares 54% amino acid (aa) sequence identity with rat PSA.

Ig Type

Rabbit IgG

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-PSA antibody

Western Blot (WB)

Application Notes for anti-PSA antibody

Western Blot Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat

WB: The detection limit for PSA is approximately 0.2ng/lane under reducing conditions.
Tested Species: In-house tested species with positives results.

Other applications have not been tested. Optimal dilutions should be determined by end users.

Product References and Citations for anti-PSA antibody

1. Balk SP, Ko YJ, Bubley GJ (January 2003). "Biology of prostate-specific antigen". J. Clin. Oncol. 21 (2): 383-91. 2. Catalona, W. J.; Richie, J. P.; Ahmann, F. R.; Hudson, M. A.; Scardino, P. T.; Flanigan, R. C.; Dekernion, J. B.; Ratliff, T. L.; Kavoussi, L. R.; Dalkin, B. L. (1994). "Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: Results of a multicenter clinical trial of 6,630 men". The Journal of urology 151 (5): 1283-1290. 3. Lilja H (November 2003). "Biology of prostate-specific antigen". Urology 62 (5 Suppl 1): 27-33. doi:10.1016/S0090-4295(03)00775-1.

Other Notes

Small volumes of anti-PSA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSA purchase

MBS178309 is an antibody targeting PSA. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for PSA. It may not necessarily be applicable to this product.

NCBI GI #

71834853

NCBI GeneID

354

NCBI Accession #

NP_001025218.1

UniProt Primary Accession #

P07288

UniProt Secondary Accession #

Q15096; Q16272; Q86TG8; Q8IXI4; C9JXH3; G3V0H4; G3XAE3

UniProt Related Accession #

P07288

GenBank Nucleotide #

NM_001030047.1

NCBI Official Full Name

prostate-specific antigen isoform 3 preproprotein

NCBI Official Synonym Full Names

kallikrein related peptidase 3

NCBI Official Symbol

KLK3

NCBI Official Synonym Symbols

APS; PSA; hK3; KLK2A1

UniProt Protein Name

Prostate-specific antigen

UniProt Synonym Protein Names

Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase

Protein Family

PsaJ-like protein asl3190

UniProt Gene Name

KLK3

UniProt Synonym Gene Names

APS; PSA; Seminin

UniProt Entry Name

KLK3_HUMAN

NCBI Protein Information

prostate-specific antigen

NCBI Summary for PSA

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for PSA

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.77; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; extracellular space; nucleus; protein complex

Molecular Function: endopeptidase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: antibacterial peptide production; cellular protein metabolic process; negative regulation of angiogenesis; proteolysis

Research Articles on PSA

1. Prostate cancer gene 3 gene expression discriminates LN metastasis and might outperform prostate specific antigen gene activity in reflecting tumor cell burden in pelvic LNs of PCa patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PSA antibody

Products by Pathway	Pathway Diagram
Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway antibodies	Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Coregulation Of Androgen Receptor Activity Pathway antibodies	Coregulation Of Androgen Receptor Activity Pathway Diagram
FOXA1 Transcription Factor Network Pathway antibodies	FOXA1 Transcription Factor Network Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Pathways In Cancer antibodies	Pathways In Cancer Diagram
Prostate Cancer Pathway antibodies	Prostate Cancer Pathway Diagram
Prostate Cancer Pathway antibodies	Prostate Cancer Pathway Diagram
Prostate Cancer Pathway antibodies	Prostate Cancer Pathway Diagram
RHO GTPase Effectors Pathway antibodies	RHO GTPase Effectors Pathway Diagram

Diseases associated with anti-PSA antibody

Disease Name	Pubmed Publications
Prostatic Neoplasms Antibodies	>101 publications with PSA and Prostatic Neoplasms
Adenocarcinoma Antibodies	>16 publications with PSA and Adenocarcinoma
Breast Neoplasms Antibodies	>15 publications with PSA and Breast Neoplasms
Disease Progression Antibodies	>14 publications with PSA and Disease Progression
Hyperplasia Antibodies	>10 publications with PSA and Hyperplasia
Prostatic Hyperplasia Antibodies	>10 publications with PSA and Prostatic Hyperplasia
Neoplasms, Experimental Antibodies	>9 publications with PSA and Neoplasms, Experimental
Neoplasm Metastasis Antibodies	>9 publications with PSA and Neoplasm Metastasis
Ovarian Neoplasms Antibodies	>7 publications with PSA and Ovarian Neoplasms
Kidney Diseases Antibodies	>6 publications with PSA and Kidney Diseases

Organs/Tissues associated with anti-PSA antibody

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>170 publications with PSA and Prostate
Brain Antibodies	>5 publications with PSA and Brain
Bone Antibodies	>5 publications with PSA and Bone
Salivary Gland Antibodies	>4 publications with PSA and Salivary Gland
Nerve Antibodies	>3 publications with PSA and Nerve
Muscle Antibodies	>2 publications with PSA and Muscle
Bone Marrow Antibodies	>2 publications with PSA and Bone Marrow
Adrenal Gland Antibodies	>1 publications with PSA and Adrenal Gland