Anti-MAD2L1 Polyclonal Antibody – Cat# MBS178326

Anti- Mad2L1 Picoband antibody, MBS178326, Western blotting
All lanes: Anti Mad2L1 (MBS178326) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD

MAD2L1 Antibody – Polyclonal

Name: MAD2L1 Antibody; Polyclonal; Human-Reactive; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS178326
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178326

Description: Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD2A(MAD2L1) detection. Tested with WB in Human.Background: Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Mad2L1, Polyclonal Antibody

Full Product Name

Anti-Mad2L1 Antibody

Product Gene Name

anti-MAD2L1 antibody

Product Synonym Names

Mitotic spindle assembly checkpoint protein MAD2A; Homolog of MAD2 S cerevisiae; HsMAD 2; HsMAD2; Human mitotic feedback control protein Madp2; MAD 2; MAD 2A; MAD2; MAD2 like 1; MAD2 like protein 1; MAD2 mitotic arrest deficient like 1; MAD2-like protein 1; MAD2A; Mad2l1; MD2L1_HUMAN; Mitotic arrest deficient 2-like protein 1; Mitotic arrest deficient like 1; Mitotic arrest deficient yeast homolog; Mitotic arrest deficient yeast homolog like 1; Mitotic spindle assembly checkpoint protein; Mitotic spindle assembly checkpoint protein MAD2A; MAD2 mitotic arrest deficient-like 1 (yeast)

Clonality

Polyclonal

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen Affinity Purified

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

10,335 Da

OMIM

601467

3D Structure

ModBase 3D Structure for Q13257

Immunogen

E Coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.

Ig Type

Rabbit IgG

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-MAD2L1 antibody

Western Blot (WB)

Application Notes for anti-MAD2L1 antibody

Western Blot Concentration: 0.1-0.5ug/ml

Product References and Citations for anti-MAD2L1 antibody

1. Cahill, D. P., da Costa, L. T., Carson-Walter, E. B., Kinzler, K. W., Vogelstein, B., Lengauer, C.Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58: 181-187, 1999. 2. "Entrez Gene: MAD2L1 MAD2 mitotic arrest deficient-like 1 (yeast)"

Other Notes

Small volumes of anti-MAD2L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAD2L1 purchase

MBS178326 is an antibody targeting MAD2L1. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for MAD2L1. It may not necessarily be applicable to this product.

NCBI GI #

4505067

NCBI GeneID

4085

NCBI Accession #

NP_002349.1

UniProt Primary Accession #

Q13257

UniProt Secondary Accession #

Q53F56; Q548X9; Q6IRW7; Q8IZX3

UniProt Related Accession #

Q13257

GenBank Nucleotide #

NM_002358.3

NCBI Official Full Name

mitotic spindle assembly checkpoint protein MAD2A

NCBI Official Synonym Full Names

MAD2 mitotic arrest deficient-like 1 (yeast)

NCBI Official Symbol

MAD2L1

NCBI Official Synonym Symbols

MAD2; HSMAD2

UniProt Protein Name

Mitotic spindle assembly checkpoint protein MAD2A

UniProt Synonym Protein Names

Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1

Protein Family

Mitotic spindle assembly checkpoint protein

UniProt Gene Name

MAD2L1

UniProt Synonym Gene Names

MAD2; HsMAD2; MAD2-like protein 1

UniProt Entry Name

MD2L1_HUMAN

NCBI Protein Information

mitotic spindle assembly checkpoint protein MAD2A

NCBI Summary for MAD2L1

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

UniProt Comments for MAD2L1

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: cytosol; kinetochore; nuclear pore; nucleus; perinuclear region of cytoplasm; spindle pole

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic sister chromatid segregation; negative regulation of apoptosis; negative regulation of mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; sister chromatid cohesion

Research Articles on MAD2L1

1. We also show that replication perturbations result in relocalization of MAD1/MAD2 in human cells, suggesting that the role of SAC in DNA repair is conserved.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAD2L1 antibody

Reference Product	PubMed Publications
CDC20 antibody	>115 publications with MAD2L1 and CDC20
MAD1L1 antibody	>64 publications with MAD2L1 and MAD1L1
BUB1B antibody	>31 publications with MAD2L1 and BUB1B
BUB3 antibody	>27 publications with MAD2L1 and BUB3
MAD2L1BP antibody	>23 publications with MAD2L1 and MAD2L1BP
CDC27 antibody	>1 publications with MAD2L1 and CDC27
CDC16 antibody	>1 publications with MAD2L1 and CDC16

Pathways associated with anti-MAD2L1 antibody

Products by Pathway	Pathway Diagram
APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies	APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies	APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies	APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway antibodies	Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway Diagram
Amplification Of Signal From The Kinetochores Pathway antibodies	Amplification Of Signal From The Kinetochores Pathway Diagram
Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway antibodies	Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram

Diseases associated with anti-MAD2L1 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>31 publications with MAD2L1 and Adenocarcinoma
Cell Transformation, Neoplastic Antibodies	>20 publications with MAD2L1 and Cell Transformation, Neoplastic
Breast Neoplasms Antibodies	>20 publications with MAD2L1 and Breast Neoplasms
Ovarian Neoplasms Antibodies	>13 publications with MAD2L1 and Ovarian Neoplasms
Lung Neoplasms Antibodies	>13 publications with MAD2L1 and Lung Neoplasms
Carcinoma, Hepatocellular Antibodies	>12 publications with MAD2L1 and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>12 publications with MAD2L1 and Liver Diseases
Neoplasms, Experimental Antibodies	>11 publications with MAD2L1 and Neoplasms, Experimental
Liver Neoplasms Antibodies	>10 publications with MAD2L1 and Liver Neoplasms
Carcinoma, Squamous Cell Antibodies	>10 publications with MAD2L1 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-MAD2L1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>19 publications with MAD2L1 and Lung
Bone Antibodies	>14 publications with MAD2L1 and Bone
Blood Antibodies	>14 publications with MAD2L1 and Blood
Stomach Antibodies	>13 publications with MAD2L1 and Stomach
Liver Antibodies	>12 publications with MAD2L1 and Liver
Bone Marrow Antibodies	>9 publications with MAD2L1 and Bone Marrow
Prostate Antibodies	>7 publications with MAD2L1 and Prostate
Skin Antibodies	>7 publications with MAD2L1 and Skin
Lymph Antibodies	>6 publications with MAD2L1 and Lymph
Kidney Antibodies	>5 publications with MAD2L1 and Kidney