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MGMT Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178332

Description: Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB, IHC-P, ICC in Human.Background: O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in… More details

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Product Overview

Online Datasheet in PDF
Product Name
MGMT, Polyclonal Antibody
Full Product Name
Anti-MGMT Antibody
Product Gene Name
anti-MGMT antibody
Product Synonym Names
Methylated-DNA--protein-cysteine methyltransferase ; 6 O methylguanine DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; Agat; AGT; AI267024; Methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT; MGMT_HUMAN; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O-6-methylguanine-DNA methyltransferase
Clonality
Polyclonal
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
21,646 Da
OMIM
156569
3D Structure
ModBase 3D Structure for P16455
Immunogen
E Coli-derived human MGMT recombinant protein (Position: D2-N207). Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MGMT antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes for anti-MGMT antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Immunocytochemistry Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-MGMT antibody

Anti- MGMT Picoband antibody, MBS178332, Western blotting
All lanes: Anti MGMT (MBS178332) at 0.5ug/ml
WB: Recombinant Human MGMT Protein 0.5ng
Predicted bind size: 21KD
Observed bind size: 21KD
Anti-MGMT Polyclonal Antibody – Cat# MBS178332

Western Blot (WB) of anti-MGMT antibody

Anti- MGMT Picoband antibody, MBS178332, Western blotting
All lanes: Anti MGMT (MBS178332) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
Anti-MGMT Polyclonal Antibody, Western Blot (WB) – Cat# MBS178332

Immunohistochemistry (IHC) of anti-MGMT antibody

Anti- MGMT Picoband antibody, MBS178332, IHC(P)
IHC(P): Human Placenta Tissue
Anti-MGMT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178332

Product References and Citations for anti-MGMT antibody

1. Hegi, M. E., Diserens, A.-C., Godard, S., Dietrich, P.-Y., Regli, L., Ostermann, S., Otten, P., Van Melle, G., de Tribolet, N., Stupp, R. Clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide. Clin. Cancer Res. 10: 1871-1874, 2004. 2. Natarajan AT, Vermeulen S, Darroudi F, Valentine MB, Brent TP, Mitra S, Tano K (January 1992). "Chromosomal localization of human O6-methylguanine-DNA methyltransferase (MGMT) gene by in situ hybridization". Mutagenesis 7 (1): 83-5. 3. Shiraishi A, Sakumi K, Sekiguchi M (October 2000). "Increased susceptibility to chemotherapeutic alkylating agents of mice deficient in DNA repair methyltransferase". Carcinogenesis 21 (10): 1879-1883.

Other Notes

Small volumes of anti-MGMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MGMT purchase

MBS178332 is an antibody targeting MGMT. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for MGMT. It may not necessarily be applicable to this product.

NCBI GI #
530394832
NCBI GeneID
4255
NCBI Accession #
XP_005252739.1
UniProt Primary Accession #
P16455
UniProt Secondary Accession #
Q5VY78
UniProt Related Accession #
P16455
GenBank Nucleotide #
XM_005252682.2
NCBI Official Full Name
methylated-DNA--protein-cysteine methyltransferase isoform X2
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
MGMT
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
UniProt Synonym Protein Names
6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Protein Family
Probable methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name
MGMT
UniProt Synonym Gene Names
MGMT
UniProt Entry Name
MGMT_HUMAN
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase

NCBI Summary for MGMT

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
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UniProt Comments for MGMT

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: Nuclear receptor co-regulator; Methyltransferase; DNA repair, damage; EC 2.1.1.63; Apoptosis

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: membrane; nucleoplasm; nucleus

Molecular Function: calcium ion binding; damaged DNA binding; DNA binding; DNA-methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity

Biological Process: DNA dealkylation; DNA ligation; DNA methylation; negative regulation of apoptosis; regulation of caspase activity; response to drug; response to ethanol; response to folic acid; response to toxin
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Research Articles on MGMT

1. The results generated by physical-functional clustering of protein indicated that the identified mutation in the vicinity of the active site of MGMT protein causes the local and global destabilization of a protein.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MGMT antibody

 Reference Product  PubMed Publications
 MLH1 antibody  >250 publications with MGMT and MLH1
 TP53 antibody  >135 publications with MGMT and TP53
 KRAS antibody  >96 publications with MGMT and KRAS
 NRAS antibody  >6 publications with MGMT and NRAS
 HIF1A antibody  >5 publications with MGMT and HIF1A
 MYB antibody  >2 publications with MGMT and MYB
 UBC antibody  >2 publications with MGMT and UBC
 HRAS antibody  >1 publications with MGMT and HRAS

Pathways associated with anti-MGMT antibody

 Products by Pathway  Pathway Diagram
 DNA Damage Reversal Pathway antibodies  DNA Damage Reversal Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 MGMT-mediated DNA Damage Reversal Pathway antibodies  MGMT-mediated DNA Damage Reversal Pathway Diagram

Diseases associated with anti-MGMT antibody

 Disease Name  Pubmed Publications
 Glioma Antibodies  >1146 publications with MGMT and Glioma
 Nervous System Diseases Antibodies  >986 publications with MGMT and Nervous System Diseases
 Brain Neoplasms Antibodies  >958 publications with MGMT and Brain Neoplasms
 Glioblastoma Antibodies  >894 publications with MGMT and Glioblastoma
 Carcinoma Antibodies  >490 publications with MGMT and Carcinoma
 Recurrence Antibodies  >252 publications with MGMT and Recurrence
 Adenocarcinoma Antibodies  >186 publications with MGMT and Adenocarcinoma
 Lung Neoplasms Antibodies  >154 publications with MGMT and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >153 publications with MGMT and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >129 publications with MGMT and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-MGMT antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1067 publications with MGMT and Brain
 Blood Antibodies  >280 publications with MGMT and Blood
 Lung Antibodies  >204 publications with MGMT and Lung
 Liver Antibodies  >128 publications with MGMT and Liver
 Bone Antibodies  >115 publications with MGMT and Bone
 Stomach Antibodies  >76 publications with MGMT and Stomach
 Intestine Antibodies  >58 publications with MGMT and Intestine
 Vascular Antibodies  >58 publications with MGMT and Vascular
 Lymph Antibodies  >55 publications with MGMT and Lymph
 Skin Antibodies  >52 publications with MGMT and Skin
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