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CD8 alpha Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178342

Description: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD8 alpha, Polyclonal Antibody
Full Product Name
Anti-CD8 alpha Antibody
Product Gene Name
Product Synonym Names
T-cell surface glycoprotein CD8 alpha chain; alpha polypeptide (p32); CD 8a; CD8 alpha chain; CD8; CD8 antigen alpha chain; CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_HUMAN; Leu 2; Leu2; Leu2 T lymphocyte antigen; Ly3; LYT3; MAL; OKT8 T cell antigen; OTTHUMP00000160760; OTTHUMP00000160764; OTTHUMP00000203528; OTTHUMP00000203721; p32; T cell antigen Leu2; T cell co receptor; T cell coreceptor; T cell surface glycoprotein CD8 alpha chain; T lymphocyte differentiation antigen T8/Leu 2; T lymphocyte differentiation antigen T8/Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen; T8/Leu-2 T-lymphocyte differentiation antigen; CD8a molecule
Clonality
Polyclonal
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Specificity
No Cross reactivity with other proteins.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
30,214 Da
OMIM
186910
3D Structure
ModBase 3D Structure for P01732
Immunogen
E Coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD8 alpha antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CD8 alpha antibody
Western Blot
Concentration: 0.1-0.5ug/ml
Tested Species: Hu

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat

WB: The detection limit for CD8 alpha is approximately 0.2ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-CD8 alpha antibody

Anti- CD 8 alpha Picoband antibody, MBS178342, Western blotting
All lanes: Anti CD 8 alpha (MBS178342) at 0.5ug/ml
WB: Recombinant Human CD 8 alpha Protein 0.5ng
Predicted bind size: 29KD
Observed bind size: 29KD
Anti-CD8 alpha Polyclonal Antibody – Cat# MBS178342

Western Blot (WB) of anti-CD8 alpha antibody

Anti- CD 8 alpha Picoband antibody, MBS178342, Western blotting
All lanes: Anti CD 8 alpha (MBS178342) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Lane 3: HL-60 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 55KD
Anti-CD8 alpha Polyclonal Antibody, Western Blot (WB) – Cat# MBS178342

Immunohistochemistry (IHC) of anti-CD8 alpha antibody

Anti- CD 8 alpha Picoband antibody, MBS178342, IHC(P)
IHC(P): Human Tonsil Tissue
Anti-CD8 alpha Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178342

Product References and Citations for anti-CD8 alpha antibody

1. Bowcock, A. M., Kavathas, P., Margolskee, R. F., Herzenberg, L., Cavalli-Sforza, L. L. An RFLP associated with pcDLeu2-14, a human T-cell differentiation antigen CD8 (Leu2) cDNA mapped to 2p12. Nucleic Acids Res. 14: 7817 only, 1986. 2. "Entrez Gene: CD8A CD8a molecule"

Other Notes

Small volumes of anti-CD8 alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD8 alpha purchase

MBS178342 is an antibody targeting CD8 alpha. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for CD8 alpha. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain isoform 1
NCBI Official Synonym Full Names
CD8a molecule
NCBI Official Symbol
CD8A
NCBI Official Synonym Symbols
CD8; MAL; p32; Leu2
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names
T-lymphocyte differentiation antigen T8/Leu-2; CD_antigen: CD8a
UniProt Gene Name
CD8A
UniProt Synonym Gene Names
MAL
UniProt Entry Name
CD8A_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain

NCBI Summary for CD8 alpha

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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UniProt Comments for CD8 alpha

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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