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ACTA1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178343

Description: Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Actin (ACTA1), Polyclonal Antibody
Full Product Name
Anti-Actin Antibody
Product Gene Name
anti-ACTA1 antibody
Product Synonym Names
Actin; ACTA; ACTA1; Actin alpha skeletal muscle; Actin; actin, alpha 1, skeletal muscle 1; actin, alpha 1, skeletal muscle; Actin, alpha skeletal muscle; actina; actine; ACTS_HUMAN; aktin; Alpha Actin 1; Alpha skeletal muscle Actin; alpha skeletal muscle; alpha-actin; Alpha-actin-1; ASMA; CFTD; CFTD1antibody; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3; actin, alpha 1, skeletal muscle
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
42,051 Da
OMIM
102610
3D Structure
ModBase 3D Structure for P68133
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ACTA1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-ACTA1 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-ACTA1 antibody

Anti- ACTIN antibody, Western blotting
All lanes: Anti ACTIN at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
Anti-ACTA1 Polyclonal Antibody – Cat# MBS178343

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Anti- ACTIN antibody, IHC(P)
IHC(P): Mouse Intestine Tissue
Anti-ACTA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178343

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Anti- ACTIN antibody, IHC(P)
IHC(P): Rat Spleen Tissue
Anti-ACTA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178343

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Anti- ACTIN antibody, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-ACTA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178343

Product References and Citations for anti-ACTA1 antibody

1. Gunning,P., Ponte,P., Okayama,H., Engel,J., Blau,H. and Kedes,L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.Mol. Cell. Biol.1983; 3 (5), 787-795. 2. Goebel,H.H., Brockmann,K., Bonnemann,C.G., Warlo,I.A., Hanefeld,F.,Labeit,S., Durling,H.J. and Laing,N.G. Actin- related myopathy without any missense mutation in the ACTA1 Gene.J. Child Neurol.2004; 19 (2), 149-153. 3. Laing,N.G., Clarke,N.F., Dye,D.E., Liyanage,K., Walker,K.R.,Kobayashi,Y., Shimakawa,S., Hagiwara,T., Ouvrier,R., Sparrow,J.C., Nishino,I., North,K.N. and Nonaka,I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694.

Other Notes

Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTA1 purchase

MBS178343 is an antibody targeting ACTA1. Also known as Anti-Actin Antibody. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.

NCBI GI #
4501881
NCBI GeneID
58
NCBI Accession #
NP_001091.1
UniProt Primary Accession #
P68133
UniProt Secondary Accession #
P02568; P99020; Q5T8M9
UniProt Related Accession #
P68133
GenBank Nucleotide #
NM_001100.3
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Synonym Protein Names
Alpha-actin-1
Protein Family
Actin
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
NCBI Protein Information
actin, alpha skeletal muscle

NCBI Summary for ACTA1

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
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UniProt Comments for ACTA1

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Cell development/differentiation; Contractile; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: actin cytoskeleton; actin filament; cytosol; extracellular space; filopodium; lamellipodium; sarcomere; stress fiber; striated muscle thin filament

Molecular Function: ADP binding; ATP binding; myosin binding; protein binding; structural constituent of cytoskeleton

Biological Process: cell growth; muscle contraction; muscle filament sliding; muscle thin filament assembly; response to extracellular stimulus; response to lithium ion; response to mechanical stimulus; response to steroid hormone stimulus; skeletal muscle fiber adaptation; skeletal muscle fiber development

Disease: Myopathy, Congenital, With Fiber-type Disproportion; Nemaline Myopathy 3
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Research Articles on ACTA1

1. The authors propose that Lpd delivers Ena/VASP proteins to growing barbed ends and increases their actin polymerase activity by tethering them to actin filaments.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACTA1 antibody

 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
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 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 RhoA Signaling Pathway antibodies  RhoA Signaling Pathway Diagram
 Signaling Events Mediated By Focal Adhesion Kinase Pathway antibodies  Signaling Events Mediated By Focal Adhesion Kinase Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram

Diseases associated with anti-ACTA1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >101 publications with ACTA1 and Nervous System Diseases
 MYOPATHY, CONGENITAL, WITH FIBER-TYPE DISPROPORTION Antibodies  >78 publications with ACTA1 and MYOPATHY, CONGENITAL, WITH FIBER-TYPE DISPROPORTION
 Heart Diseases Antibodies  >24 publications with ACTA1 and Heart Diseases
 Hypertrophy Antibodies  >23 publications with ACTA1 and Hypertrophy
 Cardiovascular Diseases Antibodies  >22 publications with ACTA1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >15 publications with ACTA1 and Disease Models, Animal
 Fibrosis Antibodies  >9 publications with ACTA1 and Fibrosis
 Inflammation Antibodies  >6 publications with ACTA1 and Inflammation
 Brain Diseases Antibodies  >6 publications with ACTA1 and Brain Diseases
 Liver Diseases Antibodies  >2 publications with ACTA1 and Liver Diseases

Organs/Tissues associated with anti-ACTA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >148 publications with ACTA1 and Muscle
 Heart Antibodies  >38 publications with ACTA1 and Heart
 Brain Antibodies  >15 publications with ACTA1 and Brain
 Embryonic Tissue Antibodies  >8 publications with ACTA1 and Embryonic Tissue
 Bone Antibodies  >7 publications with ACTA1 and Bone
 Lung Antibodies  >7 publications with ACTA1 and Lung
 Nerve Antibodies  >6 publications with ACTA1 and Nerve
 Vascular Antibodies  >4 publications with ACTA1 and Vascular
 Eye Antibodies  >3 publications with ACTA1 and Eye
 Liver Antibodies  >3 publications with ACTA1 and Liver
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