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CD33 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178407

CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells.… More details

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Product Overview

Online Datasheet in PDF
Product Name
CD33, Polyclonal Antibody
Full Product Name
Anti-CD33 Antibody
Product Gene Name
anti-CD33 antibody
Product Synonym Names
CD 33; CD33; CD33 antigen (gp67); CD33 antigen; CD33 molecule; gp67; My9; p67; SIGLEC 3; SIGLEC3; Siglec-3; Q63994; Myeloid cell surface antigen CD33; CD33 molecule
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for Q63994
Immunogen
E Coli-derived mouse CD33 recombinant protein (Position: D18-E240). Mouse CD33 shares 61.6% amino acid (aa) sequence identity with human CD33.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD33 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin-embedded Section
Application Notes for anti-CD33 antibody
Western Blot -
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse
Antigen Retrieval: -

Immunohistochemistry (Paraffin-embedded Section) -
Concentration: 0.5-1ug/ml
Tested Species: Mouse
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-CD33 antibody

Western blot analysis of CD33 expression in mosue lung extract (lane 1). CD33 at 45KD was detected using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-CD33 Polyclonal Antibody – Cat# MBS178407

Immunohistochemistry (IHC) of anti-CD33 antibody

CD33 was detected in paraffin-embedded sections of mouse pleen tissues using rabbit anti- CD33 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-CD33 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178407

Product References and Citations for anti-CD33 antibody

1. Adriaansen, H. J., Geurts Van Kessel, A. H. M., Wijdenes-De Bresser, J. H. F. M., Van Drunen-Schoenmaker, E., Van Dongen, J. J. M.Expression of the myeloid differentiation antigen CD33 depends on the presence of human chromosome 19 in human-mouse hybrids. Ann. Hum. Genet. 54: 115-119, 1990.
2. Brinkman-Van der Linden, E. C. M., Angata, T., Reynolds, S. A., Powell, L. D., Hedrick, S. M., Varki, A. CD33/Siglec-3 binding specificity, expression pattern, and consequences of gene deletion in mice. Molec. Cell. Biol. 23: 4199-4206, 2003.
3. Trask, B., Fertitta, A., Christensen, M., Youngblom, J., Bergmann, A., Copeland, A., de Jong, P., Mohrenweiser, H., Olsen, A., Carrano, A., Tynan, K. Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers. Genomics 15: 133-145, 1993.

Other Notes

Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD33 purchase

MBS178407 is an antibody targeting CD33. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.

NCBI GI #
161484603
NCBI GeneID
12489
NCBI Accession #
NP_001104528.1
UniProt Primary Accession #
Q63994
UniProt Secondary Accession #
Q63997; A2RT59
UniProt Related Accession #
Q63994
GenBank Nucleotide #
NM_001111058.1
NCBI Official Full Name
myeloid cell surface antigen CD33 isoform 1
NCBI Official Synonym Full Names
CD33 antigen
NCBI Official Symbol
Cd33
NCBI Official Synonym Symbols
gp67; Siglec-3
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Synonym Protein Names
Sialic acid-binding Ig-like lectin 3; Siglec-3; CD_antigen: CD33
Protein Family
Myeloid cell surface antigen
UniProt Gene Name
Cd33
UniProt Synonym Gene Names
Siglec3; Siglec-3
UniProt Entry Name
CD33_MOUSE
NCBI Protein Information
myeloid cell surface antigen CD33

UniProt Comments for CD33

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: external side of plasma membrane
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Research Articles on CD33

1. Lectin galactoside-binding soluble 3 binding protein (LGALS3BP) is a tumor-associated immunomodulatory ligand for CD33-related Siglecs.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD33 antibody

 Reference Product  PubMed Publications
 Cd86 antibody  >50 publications with CD33 and Cd86

Pathways associated with anti-CD33 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram

Diseases associated with anti-CD33 antibody

 Disease Name  Pubmed Publications
 Leukemia, Myeloid, Acute Antibodies  >1233 publications with CD33 and Leukemia, Myeloid, Acute
 Necrosis Antibodies  >97 publications with CD33 and Necrosis
 Inflammation Antibodies  >86 publications with CD33 and Inflammation
 Cardiovascular Diseases Antibodies  >85 publications with CD33 and Cardiovascular Diseases
 Respiratory Tract Diseases Antibodies  >54 publications with CD33 and Respiratory Tract Diseases
 Alzheimer Disease Antibodies  >50 publications with CD33 and Alzheimer Disease
 Breast Neoplasms Antibodies  >43 publications with CD33 and Breast Neoplasms
 Lung Diseases Antibodies  >41 publications with CD33 and Lung Diseases
 Skin Neoplasms Antibodies  >29 publications with CD33 and Skin Neoplasms
 Lung Neoplasms Antibodies  >24 publications with CD33 and Lung Neoplasms

Organs/Tissues associated with anti-CD33 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >1028 publications with CD33 and Bone Marrow
 Skin Antibodies  >67 publications with CD33 and Skin
 Brain Antibodies  >42 publications with CD33 and Brain
 Embryonic Tissue Antibodies  >38 publications with CD33 and Embryonic Tissue
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