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CD9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178430

Rabbit IgG polyclonal antibody for CD9 antigen (CD9) detection. Background: CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD9, Polyclonal Antibody
Full Product Name
Anti-CD9 Antibody
Product Gene Name
anti-CD9 antibody
Product Synonym Names
Tetraspanin 29; Tetraspanin-29; 5H9; 5H9 antigen; BA2; BTCC 1; BTCC1; CD9; CD 9; CD9 antigen; CD9 antigen p24; CD9 molecule; DRAP 27; DRAP27; GIG2; MIC3; MRP 1; MRP-1; MRP1; p24; p24 antigen; Tspan 29; Tspan-29; TSPAN29; P40240; CD9 antigen; CD9 molecule
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
25,258 Da
3D Structure
ModBase 3D Structure for P40240
Immunogen
E Coli-derived mouse CD9 recombinant protein (Position: T110-I193). Mouse CD9 shares 77.4% and 86.9% amino acid (aa) sequence identity with human and rat CD9, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD9 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CD9 antibody
Western Blot: 0.1-0.5mug/ml; Tested Species: Mouse
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Mouse
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western Blot (WB) of anti-CD9 antibody

Western blot analysis of CD9 expression in mouse kidney extract (lane 1). CD9 at 25KD was detected using rabbit anti- CD9 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-CD9 Polyclonal Antibody – Cat# MBS178430

Immunohistochemistry (IHC) of anti-CD9 antibody

CD9 was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- CD9 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-CD9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178430

Product References and Citations for anti-CD9 antibody

1. Katz F, Povey S, Parkar M, Schneider C, Sutherland R, Stanley K, Solomon E, Greaves M (March 1984). "Chromosome assignment of monoclonal antibody-defined determinants on human leukemic cells". Eur J Immunol 13(12): 1008-1013.
2. Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C (2000). "Severely Reduced Female Fertility in CD9-Deficient Mice". Science 287(5451): 319-321.
3. Runge KE, Evans JE, He ZY, Gupta S, McDonald KL, Stahlberg H, Primakoff P, Myles DG (2007). "Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution".Developmental Biology 304 (1): 317-325.

Other Notes

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD9 purchase

MBS178430 is an antibody targeting CD9. Reactive in Mouse
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD9. It may not necessarily be applicable to this product.

NCBI GI #
6680894
NCBI GeneID
12527
NCBI Accession #
NP_031683.1
UniProt Primary Accession #
P40240
UniProt Secondary Accession #
Q3U9W0
UniProt Related Accession #
P40240
GenBank Nucleotide #
NM_007657.3
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 antigen
NCBI Official Symbol
Cd9
NCBI Official Synonym Symbols
Tspan29
UniProt Protein Name
CD9 antigen
UniProt Synonym Protein Names
CD_antigen: CD9
Protein Family
CD9 antigen
UniProt Gene Name
Cd9
UniProt Entry Name
CD9_MOUSE
NCBI Protein Information
CD9 antigen

UniProt Comments for CD9

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass

Cellular Component: apical plasma membrane; cell surface; external side of plasma membrane; extracellular space; focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: cell adhesion; cell motility; cell surface receptor linked signal transduction; fusion of sperm to egg plasma membrane; negative regulation of cell proliferation; paranodal junction assembly; receptor internalization
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Research Articles on CD9

1. upregulation may play an important role in podocyte morphology, adhesion, and migration

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD9 antibody

 Reference Product  PubMed Publications
 Cd36 antibody  >39 publications with CD9 and Cd36
 Kit antibody  >26 publications with CD9 and Kit
 Izumo1 antibody  >16 publications with CD9 and Izumo1
 Ptgfrn antibody  >12 publications with CD9 and Ptgfrn
 Psg17 antibody  >8 publications with CD9 and Psg17
 Scarb1 antibody  >1 publications with CD9 and Scarb1
 Itgb1 antibody  >1 publications with CD9 and Itgb1

Pathways associated with anti-CD9 antibody

 Products by Pathway  Pathway Diagram
 Fertilization Pathway antibodies  Fertilization Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Reproduction Pathway antibodies  Reproduction Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Sperm:Oocyte Membrane Binding Pathway antibodies  Sperm:Oocyte Membrane Binding Pathway Diagram

Diseases associated with anti-CD9 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >70 publications with CD9 and Neoplasm Metastasis
 Neoplasms, Germ Cell and Embryonal Antibodies  >64 publications with CD9 and Neoplasms, Germ Cell and Embryonal
 Inflammation Antibodies  >56 publications with CD9 and Inflammation
 Leukemia, Myeloid, Acute Antibodies  >53 publications with CD9 and Leukemia, Myeloid, Acute
 Cardiovascular Diseases Antibodies  >48 publications with CD9 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >37 publications with CD9 and Lung Neoplasms
 Breast Neoplasms Antibodies  >36 publications with CD9 and Breast Neoplasms
 Necrosis Antibodies  >35 publications with CD9 and Necrosis
 Disease Models, Animal Antibodies  >31 publications with CD9 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >27 publications with CD9 and Neoplasms, Experimental

Organs/Tissues associated with anti-CD9 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >133 publications with CD9 and Bone Marrow
 Lung Antibodies  >89 publications with CD9 and Lung
 Embryonic Tissue Antibodies  >70 publications with CD9 and Embryonic Tissue
 Lymph Node Antibodies  >49 publications with CD9 and Lymph Node
 Kidney Antibodies  >47 publications with CD9 and Kidney
 Liver Antibodies  >42 publications with CD9 and Liver
 Muscle Antibodies  >39 publications with CD9 and Muscle
 Uterus Antibodies  >39 publications with CD9 and Uterus
 Spleen Antibodies  >39 publications with CD9 and Spleen
 Skin Antibodies  >38 publications with CD9 and Skin
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