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CD27 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178431

Rabbit IgG polyclonal antibody for CD27 antigen (CD27) detection. Background: CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70,… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD27, Polyclonal Antibody
Full Product Name
Anti-CD27 Antibody
Product Gene Name
anti-CD27 antibody
Product Synonym Names
CD 27; CD27; CD27 antigen; CD27 molecule; CD27L receptor; LPFS2; S152; T14; TNFRSF 7; TNFRSF7; TNFSF7; Tp 55; Tp55; P26842; CD27 antigen; CD27 molecule
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
29,137 Da
OMIM
186711
3D Structure
ModBase 3D Structure for P26842
Immunogen
E Coli-derived human CD27 recombinant protein (Position: A20-R191). Human CD27 shares 62.8% amino acid (aa) sequence identity with mouse CD27.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD27 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CD27 antibody
Western Blot: 0.1-0.5mug/ml; Tested Species: Human
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western Blot (WB) of anti-CD27 antibody

Western blot analysis of CD27 expression in HELA whole cell lysates (lane 1). CD27 at 51KD was detected using rabbit anti- CD27 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-CD27 Polyclonal Antibody – Cat# MBS178431

Immunohistochemistry (IHC) of anti-CD27 antibody

CD27 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- CD27 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-CD27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178431

Flow Cytometry (FC/FACS) of anti-CD27 antibody

Figure 3. Flow Cytometry analysis of A549 cells using anti-CD27 antibody (MBS178431).
Overlay histogram showing A549 cells stained with MBS178431 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD27 Antibody (MBS178431,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-CD27 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS178431

Product References and Citations for anti-CD27 antibody

1. "Entrez Gene: CD27 CD27 molecule".
2. Prasad KV, Ao Z, Yoon Y, Wu MX, Rizk M, Jacquot S, Schlossman SF (Jun 1997). "CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein". Proceedings of the National Academy of Sciences of the United States of America 94 (12): 6346-51.
3. Yamamoto H, Kishimoto T, Minamoto S (Nov 1998). "NF-kappaB activation in CD27 signaling: involvement of TNF receptor-associated factors in its signaling and identification of functional region of CD27". Journal of Immunology 161 (9): 4753-9.

Other Notes

Small volumes of anti-CD27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD27 purchase

MBS178431 is an antibody targeting CD27. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD27. It may not necessarily be applicable to this product.

NCBI GI #
4507587
NCBI GeneID
939
NCBI Accession #
NP_001233.1
UniProt Primary Accession #
P26842
UniProt Secondary Accession #
B2RDZ0
UniProt Related Accession #
P26842
GenBank Nucleotide #
NM_001242.4
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
UniProt Protein Name
CD27 antigen
UniProt Synonym Protein Names
CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD_antigen: CD27
Protein Family
CD27 antigen
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN
NCBI Protein Information
CD27 antigen

NCBI Summary for CD27

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD27

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Apoptosis; Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; neuron projection; nucleus; plasma membrane

Molecular Function: caspase inhibitor activity; protein binding; tumor necrosis factor receptor activity

Biological Process: brain development; immune response; induction of apoptosis via death domain receptors; inflammatory response; negative regulation of apoptosis; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; regulation of cell proliferation; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway

Disease: Lymphoproliferative Syndrome 2
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Research Articles on CD27

1. Novel mutations in TNFRSF7/CD27 cause EBV-associated lymphoproliferative disease/hemophagocytic lymphohistiocytosis, Hodgkin lymphoma, uveitis, and recurrent infections.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD27 antibody

 Reference Product  PubMed Publications
 CD70 antibody  >286 publications with CD27 and CD70
 CD5 antibody  >131 publications with CD27 and CD5
 IL10 antibody  >98 publications with CD27 and IL10
 CXCR3 antibody  >55 publications with CD27 and CXCR3
 TRAF2 antibody  >13 publications with CD27 and TRAF2
 SIVA1 antibody  >12 publications with CD27 and SIVA1
 TRAF3 antibody  >8 publications with CD27 and TRAF3
 CCR3 antibody  >4 publications with CD27 and CCR3
 CD40LG antibody  >1 publications with CD27 and CD40LG

Pathways associated with anti-CD27 antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 TNFR2 Non-canonical NF-kB Pathway antibodies  TNFR2 Non-canonical NF-kB Pathway Diagram
 TNFs Bind Their Physiological Receptors Pathway antibodies  TNFs Bind Their Physiological Receptors Pathway Diagram

Diseases associated with anti-CD27 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >988 publications with CD27 and Immune System Diseases
 Necrosis Antibodies  >452 publications with CD27 and Necrosis
 Inflammation Antibodies  >205 publications with CD27 and Inflammation
 Nervous System Diseases Antibodies  >115 publications with CD27 and Nervous System Diseases
 Vascular Diseases Antibodies  >96 publications with CD27 and Vascular Diseases
 Carcinoma Antibodies  >71 publications with CD27 and Carcinoma
 Memory Disorders Antibodies  >48 publications with CD27 and Memory Disorders
 Neoplasms, Experimental Antibodies  >42 publications with CD27 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >42 publications with CD27 and Kidney Diseases
 Cell Transformation, Neoplastic Antibodies  >23 publications with CD27 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-CD27 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2556 publications with CD27 and Blood
 Lymph Node Antibodies  >188 publications with CD27 and Lymph Node
 Spleen Antibodies  >169 publications with CD27 and Spleen
 Lymph Antibodies  >151 publications with CD27 and Lymph
 Lung Antibodies  >95 publications with CD27 and Lung
 Kidney Antibodies  >93 publications with CD27 and Kidney
 Thymus Antibodies  >88 publications with CD27 and Thymus
 Tonsil Antibodies  >71 publications with CD27 and Tonsil
 Heart Antibodies  >44 publications with CD27 and Heart
 Intestine Antibodies  >43 publications with CD27 and Intestine
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