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ISG15 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178451

Rabbit IgG polyclonal antibody for Ubiquitin-like protein ISG15 (ISG15) detection. Background: Interferon-stimulated gene 15 (ISG15) is a 17 kDA secreted protein that in humans is encoded by the ISG15 gene. The protein encoded by this gene is a ubiquitin-like… More details

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Product Overview

Online Datasheet in PDF
Product Name
ISG15, Polyclonal Antibody
Full Product Name
Anti-ISG15 Antibody
Product Gene Name
anti-ISG15 antibody
Product Synonym Names
G1P2; hUCRP; IFI 15; IFI15; IP17; ISG 15; ISG15; UCRP; Q64339; Ubiquitin-like protein ISG15; ISG15 ubiquitin-like modifier
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
17,898 Da
3D Structure
ModBase 3D Structure for Q64339
Immunogen
E Coli-derived mouse ISG15 recombinant protein (Position: A2-G155). Mouse ISG15 shares 65.8% amino acid (aa) sequence identity with human ISG15.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ISG15 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-ISG15 antibody
Western Blot: 0.1-0.5mug/ml; Tested Species: Mouse
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Mouse
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western Blot (WB) of anti-ISG15 antibody

Figure 1. Western blot analysis of ISG15/Ucrp using anti-ISG15/Ucrp antibody (MBS178451).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hours at RT. The membrane was incubated with rabbit anti-ISG15/Ucrp antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ISG15/Ucrp at approximately 15KD. The expected band size for ISG15/Ucrp is at 17KD.
Rabbit Anti-ISG15 Polyclonal Antibody – Cat# MBS178451

Immunohistochemistry (IHC) of anti-ISG15 antibody

Figure 2. IHC analysis of ISG15/Ucrp using anti- ISG15/Ucrp antibody (MBS178451).
ISG15/Ucrp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- ISG15/Ucrp Antibody (MBS178451) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-ISG15 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178451

Product References and Citations for anti-ISG15 antibody

1. "Entrez Gene: ISG15 ISG15 ubiquitin-like modifier".
2. Blomstrom DC, Fahey D, Kutny R, Korant BD, Knight E Jr (Aug 1986). "Molecular characterization of the interferon-induced 15-kDa protein. Molecular cloning and nucleotide and amino acid sequence". J Biol Chem261 (19): 8811-6.
3. Morales, David J.; Lenschow, Deborah J. (December 2013). "The Antiviral Activities of ISG15".Journal of Molecular Biology 425 (24): 4995-5008.

Other Notes

Small volumes of anti-ISG15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ISG15 purchase

MBS178451 is an antibody targeting ISG15. Reactive in Mouse
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ISG15. It may not necessarily be applicable to this product.

NCBI GI #
226874851
NCBI GeneID
100038882
NCBI Accession #
NP_056598.2
UniProt Primary Accession #
Q64339
UniProt Secondary Accession #
Q8K0H3
UniProt Related Accession #
Q64339
GenBank Nucleotide #
NM_015783.3
NCBI Official Full Name
ubiquitin-like protein ISG15
NCBI Official Synonym Full Names
ISG15 ubiquitin-like modifier
NCBI Official Symbol
Isg15
NCBI Official Synonym Symbols
G1p2; IP17; Irfp; UCRP; IGI15
UniProt Protein Name
Ubiquitin-like protein ISG15
UniProt Synonym Protein Names
Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein
Protein Family
Ubiquitin-like protein
UniProt Gene Name
Isg15
UniProt Synonym Gene Names
G1p2; Ucrp; IP17
UniProt Entry Name
ISG15_MOUSE
NCBI Protein Information
ubiquitin-like protein ISG15

UniProt Comments for ISG15

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Cellular Component: extracellular region

Molecular Function: protein binding; protein tag

Biological Process: defense response to bacterium; defense response to virus; ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of erythrocyte differentiation; regulation of interferon-gamma production
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Research Articles on ISG15

1. These studies provide evidence, ISG15 is elevated following TBI in mice, preceding MLCK elevation, development of BBB disruption, and cerebral edema.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ISG15 antibody

 Reference Product  PubMed Publications
 Stat1 antibody  >85 publications with ISG15 and Stat1
 Usp18 antibody  >78 publications with ISG15 and Usp18
 Ifit1 antibody  >36 publications with ISG15 and Ifit1
 Mx2 antibody  >27 publications with ISG15 and Mx2
 Ddx58 antibody  >20 publications with ISG15 and Ddx58
 Ube2l6 antibody  >14 publications with ISG15 and Ube2l6
 Herc6 antibody  >7 publications with ISG15 and Herc6
 Nedd4 antibody  >3 publications with ISG15 and Nedd4
 Uba7 antibody  >3 publications with ISG15 and Uba7
 Ube2n antibody  >1 publications with ISG15 and Ube2n

Pathways associated with anti-ISG15 antibody

 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DNA Damage Bypass Pathway antibodies  DNA Damage Bypass Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 Termination Of Translesion DNA Synthesis Pathway antibodies  Termination Of Translesion DNA Synthesis Pathway Diagram

Diseases associated with anti-ISG15 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >57 publications with ISG15 and Liver Diseases
 Inflammation Antibodies  >38 publications with ISG15 and Inflammation
 Influenza, Human Antibodies  >31 publications with ISG15 and Influenza, Human
 Nervous System Diseases Antibodies  >30 publications with ISG15 and Nervous System Diseases
 Skin Diseases Antibodies  >29 publications with ISG15 and Skin Diseases
 Necrosis Antibodies  >28 publications with ISG15 and Necrosis
 Kidney Diseases Antibodies  >20 publications with ISG15 and Kidney Diseases
 Disease Models, Animal Antibodies  >19 publications with ISG15 and Disease Models, Animal
 Lung Diseases Antibodies  >16 publications with ISG15 and Lung Diseases
 Breast Neoplasms Antibodies  >16 publications with ISG15 and Breast Neoplasms

Organs/Tissues associated with anti-ISG15 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >30 publications with ISG15 and Brain
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