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SLC1A3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178459

Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. It is mapped… More details

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Product Overview

Online Datasheet in PDF
Product Name
EAAT1 (SLC1A3), Polyclonal Antibody
Full Product Name
Anti-EAAT1 Antibody
Product Gene Name
anti-SLC1A3 antibody
Product Synonym Names
EA6; EAA1; EAAT 1; EAAT1; EAAT1; GLAST; GLAST1; Glast; GLAST-1; SLC1A3; P43003 ; Excitatory amino acid transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
600111
3D Structure
ModBase 3D Structure for P43003
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT1 (14-42aa RMERFQQGVRKRTLLAKKKVQNITKEDVK), different from the related mouse sequence by three amino acids, and identical to the related rat sequence.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SLC1A3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-SLC1A3 antibody
Western Blot (WB)
Concentration: 0.1-0.5ug/ml
Tested Species: Mouse, Rat
Predicted Species: Human
Antigen Retrieval: -

Immunohistochemistry (IHC) Paraffin
Concentration: 0.5-1ug/ml
Tested Species: Human, Mouse, Rat
Predicted Species: -
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested. Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-SLC1A3 antibody

Western blot analysis of EAAT1 expression in rat brain extract (lane 1) and mouse brain extract (lane 2). EAAT1 at 60KD was detected using rabbit anti-EAAT1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-SLC1A3 Polyclonal Antibody – Cat# MBS178459

Immunohistochemistry (IHC) of anti-SLC1A3 antibody

EAAT1 was detected in paraffin-embedded sections of mouse cerebellum tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-SLC1A3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178459

Immunohistochemistry (IHC) of anti-SLC1A3 antibody

EAAT1 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-SLC1A3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178459

Immunohistochemistry (IHC) of anti-SLC1A3 antibody

EAAT1 was detected in paraffin-embedded sections of huaman glioma tissues using rabbit anti- EAAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-SLC1A3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178459

Product References and Citations for anti-SLC1A3 antibody

1. de Vries, B., Mamsa, H., Stam, A. H., Wan, J., Bakker, S. L. M., Vanmolkot, K. R. J., Haan, J., Terwindt, G. M., Boon, E. M. J., Howard, B. D., Frants, R. R., Baloh, R. W., Ferrari, M. D., Jen, J. C., van den Maagdenberg, A. M. J. M. Episodic ataxia associated with EAAT1 mutation C186S affecting glutamate reuptake. Arch. Neurol. 66: 97-101, 2009. Note: Errata: Arch. Neurol. 66: 497 only, 2009; Arch. Neurol. 66: 772 only, 2009.
2. Matsugami, T. R., Tanemura, K., Mieda, M., Nakatomi, R., Yamada, K., Kondo, T., Ogawa, M., Obata, K., Watanabe, M., Hashikawa, T., Tanaka, K. Indispensability of the glutamate transporters GLAST and GLT1 to brain development. Proc. Nat. Acad. Sci. 103: 12161-12166, 2006.

Other Notes

Small volumes of anti-SLC1A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC1A3 purchase

MBS178459 is an antibody targeting SLC1A3. Also known as Anti-EAAT1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC1A3. It may not necessarily be applicable to this product.

NCBI GI #
262359914
NCBI GeneID
6507
NCBI Accession #
NP_001160167.1
UniProt Primary Accession #
P43003
UniProt Secondary Accession #
Q4JCQ8; B2R5T3
UniProt Related Accession #
P43003
GenBank Nucleotide #
NM_001166695.2
NCBI Official Full Name
excitatory amino acid transporter 1 isoform 2
NCBI Official Synonym Full Names
solute carrier family 1 member 3
NCBI Official Symbol
SLC1A3
NCBI Official Synonym Symbols
EA6; EAAT1; GLAST; GLAST1
UniProt Protein Name
Excitatory amino acid transporter 1
UniProt Synonym Protein Names
Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3
Protein Family
Excitatory amino acid transporter
UniProt Gene Name
SLC1A3
UniProt Synonym Gene Names
EAAT1; GLAST; GLAST1; GLAST-1
UniProt Entry Name
EAA1_HUMAN
NCBI Protein Information
excitatory amino acid transporter 1

NCBI Summary for SLC1A3

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
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UniProt Comments for SLC1A3

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane; plasma membrane

Molecular Function: amino acid transmembrane transporter activity; high-affinity glutamate transmembrane transporter activity; L-glutamate transmembrane transporter activity

Biological Process: glutamate secretion; ion transport; L-glutamate import; neurotransmitter uptake; positive regulation of defense response to virus by host; synaptic transmission

Disease: Episodic Ataxia, Type 6
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Research Articles on SLC1A3

1. There was no association between pyramidal cell EAAT1 splice variant expression and schizophrenia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC1A3 antibody

 Reference Product  PubMed Publications
 GLUL antibody  >6 publications with SLC1A3 and GLUL
 FABP7 antibody  >4 publications with SLC1A3 and FABP7
 GDNF antibody  >4 publications with SLC1A3 and GDNF
 EGFR antibody  >3 publications with SLC1A3 and EGFR
 SLC6A1 antibody  >3 publications with SLC1A3 and SLC6A1
 SGK1 antibody  >1 publications with SLC1A3 and SGK1

Pathways associated with anti-SLC1A3 antibody

 Products by Pathway  Pathway Diagram
 Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway antibodies  Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway Diagram
 Glutamate Neurotransmitter Release Cycle Pathway antibodies  Glutamate Neurotransmitter Release Cycle Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Release Cycle Pathway antibodies  Neurotransmitter Release Cycle Pathway Diagram
 Neurotransmitter Uptake And Metabolism In Glial Cells Pathway antibodies  Neurotransmitter Uptake And Metabolism In Glial Cells Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Synaptic Vesicle Pathway antibodies  Synaptic Vesicle Pathway Diagram

Diseases associated with anti-SLC1A3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >123 publications with SLC1A3 and Nervous System Diseases
 Brain Diseases Antibodies  >90 publications with SLC1A3 and Brain Diseases
 Disease Models, Animal Antibodies  >59 publications with SLC1A3 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >34 publications with SLC1A3 and Cardiovascular Diseases
 Epilepsy Antibodies  >22 publications with SLC1A3 and Epilepsy
 Seizures Antibodies  >15 publications with SLC1A3 and Seizures
 Nerve Degeneration Antibodies  >14 publications with SLC1A3 and Nerve Degeneration
 Inflammation Antibodies  >13 publications with SLC1A3 and Inflammation
 Movement Disorders Antibodies  >12 publications with SLC1A3 and Movement Disorders
 Ataxia Antibodies  >9 publications with SLC1A3 and Ataxia

Organs/Tissues associated with anti-SLC1A3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >282 publications with SLC1A3 and Brain
 Nerve Antibodies  >79 publications with SLC1A3 and Nerve
 Eye Antibodies  >51 publications with SLC1A3 and Eye
 Blood Antibodies  >37 publications with SLC1A3 and Blood
 Spinal Cord Antibodies  >36 publications with SLC1A3 and Spinal Cord
 Embryonic Tissue Antibodies  >20 publications with SLC1A3 and Embryonic Tissue
 Muscle Antibodies  >14 publications with SLC1A3 and Muscle
 Kidney Antibodies  >13 publications with SLC1A3 and Kidney
 Bone Antibodies  >7 publications with SLC1A3 and Bone
 Heart Antibodies  >7 publications with SLC1A3 and Heart
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