AGO2 Antibody – Rabbit Polyclonal

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: EC 3.1.26.n2; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: cytoplasm; cytosol; membrane; mRNA cap complex; nucleoplasm; nucleus; polysome; ribonucleoprotein complex

Molecular Function: double-stranded RNA binding; endoribonuclease activity; miRNA binding; protein binding; protein C-terminus binding; RNA 7-methylguanosine cap binding; single-stranded RNA binding; siRNA binding

Biological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; negative regulation of translational initiation; phosphoinositide-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; pre-microRNA processing; RNA interference, siRNA loading onto RISC; RNA secondary structure unwinding; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway