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CA2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178489

Rabbit IgG polyclonal antibody for Carbonic anhydrase 2 (CA2) detection. Background: CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CA2, Polyclonal Antibody
Full Product Name
Anti-ca2 Antibody
Product Gene Name
anti-CA2 antibody
Product Synonym Names
4.2.1.1; CA2; CAC; CAH2; CAII; CA-II; Carbonate dehydratase II; Carbonic anhydrase 2; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic Anhydrase II; Carbonic dehydratase; P00918; Carbonic anhydrase 2; carbonic anhydrase II
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
29,246 Da
OMIM
259730
3D Structure
ModBase 3D Structure for P00918
Immunogen
E Coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CA2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CA2 antibody
Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western Blot (WB) of anti-CA2 antibody

Figure 1. Western blot analysis of CA2 using anti- CA2 antibody (MBS178489).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat ovary tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: MCF-7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CA2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CA2 at approximately 29KD. The expected band size for CA2 is at 29KD‚
Rabbit Anti-CA2 Polyclonal Antibody – Cat# MBS178489

Immunohistochemistry (IHC) of anti-CA2 antibody

Figure 2. IHC analysis of CA2 using anti- CA2 antibody (MBS178489).
CA2 was detected in paraffin-embedded section of human gastric cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CA2 Antibody (MBS178489) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-CA2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178489

Immunohistochemistry (IHC) of anti-CA2 antibody

Figure 3. IHC analysis of CA2 using anti- CA2 antibody (MBS178489).
CA2 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CA2 Antibody (MBS178489) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Rabbit Anti-CA2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178489

Flow Cytometry (FC/FACS) of anti-CA2 antibody

Figure 4. Flow Cytometry analysis of HT-29 cells using anti-CA2 antibody (MBS178489).
Overlay histogram showing HT-29 cells stained with MBS178489 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CA2 Antibody (MBS178489,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-CA2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS178489

Flow Cytometry (FC/FACS) of anti-CA2 antibody

Figure 5. Flow Cytometry analysis of SW480 cells using anti-CA2 antibody (MBS178489).
Overlay histogram showing SW480 cells stained with MBS178489 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CA2 Antibody (MBS178489,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-CA2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS178489

Product References and Citations for anti-CA2 antibody

1. Aramaki, S., Yoshida, I., Yoshino, M., Kondo, M., Sato, Y., Noda, K., Jo, R., Okue, A., Sai, N., Yamashita, F. Carbonic anhydrase II deficiency in three unrelated Japanese patients. J. Inherit. Metab. Dis. 16: 982-990, 1993.
2. Laitala, T., Vaananen, H. K. Inhibition of bone resorption in vitro by antisense RNA and DNA molecules targeted against carbonic anhydrase II or two subunits of vacuolar H(+)-ATPase. J. Clin. Invest. 93: 2311-2318, 1994.
3. Venta, P. J., Welty, R. J., Johnson, T. M., Sly, W. S., Tashian, R. E. Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107his-to-tyr): complete structure of the normal human CA II gene. Am. J. Hum. Genet. 49: 1082-1090, 1991.

Other Notes

Small volumes of anti-CA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA2 purchase

MBS178489 is an antibody targeting CA2. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CA2. It may not necessarily be applicable to this product.

NCBI GI #
4557395
NCBI GeneID
760
NCBI Accession #
NP_000058.1
UniProt Primary Accession #
P00918
UniProt Secondary Accession #
Q6FI12; Q96ET9; B2R7G8
UniProt Related Accession #
P00918
GenBank Nucleotide #
NM_000067.2
NCBI Official Full Name
carbonic anhydrase 2 isoform 1
NCBI Official Synonym Full Names
carbonic anhydrase 2
NCBI Official Symbol
CA2
NCBI Official Synonym Symbols
CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
UniProt Protein Name
Carbonic anhydrase 2
UniProt Synonym Protein Names
Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
Protein Family
Beta-carotene hydroxylase
UniProt Gene Name
CA2
UniProt Synonym Gene Names
CAC; CA-II
UniProt Entry Name
CAH2_HUMAN
NCBI Protein Information
carbonic anhydrase 2

NCBI Summary for CA2

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
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UniProt Comments for CA2

CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: apical part of cell; cytoplasm; cytosol; plasma membrane

Molecular Function: arylesterase activity; carbonate dehydratase activity; protein binding; zinc ion binding

Biological Process: bicarbonate transport; regulation of intracellular pH

Disease: Osteopetrosis, Autosomal Recessive 3
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Research Articles on CA2

1. identified 2 novel mutations in 2 Chinese families with CAII deficiency syndrome; a nonsense mutation in exon 4 in both families; a splice mutation at the splice donor site of intron 3 in 1 family; the splice-site mutation causes exon 3 skipping in patient's mRNA resulting in an in-frame deletion and a novel premature stop codon
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CA2 antibody

 Reference Product  PubMed Publications
 SLC9A1 antibody  >30 publications with CA2 and SLC9A1
 SLC4A4 antibody  >6 publications with CA2 and SLC4A4
 SLC26A6 antibody  >4 publications with CA2 and SLC26A6
 SLC26A4 antibody  >4 publications with CA2 and SLC26A4
 PGR antibody  >3 publications with CA2 and PGR
 SLC4A1 antibody  >3 publications with CA2 and SLC4A1
 SLC4A2 antibody  >1 publications with CA2 and SLC4A2

Pathways associated with anti-CA2 antibody

 Products by Pathway  Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway antibodies  Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway Diagram
 Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway antibodies  Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram

Diseases associated with anti-CA2 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >7024 publications with CA2 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >4898 publications with CA2 and Nervous System Diseases
 Heart Diseases Antibodies  >4229 publications with CA2 and Heart Diseases
 Hypertension Antibodies  >2363 publications with CA2 and Hypertension
 Inflammation Antibodies  >1455 publications with CA2 and Inflammation
 Necrosis Antibodies  >1427 publications with CA2 and Necrosis
 Hypertrophy Antibodies  >1237 publications with CA2 and Hypertrophy
 Kidney Diseases Antibodies  >813 publications with CA2 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >775 publications with CA2 and Neoplasms, Experimental
 Fibrosis Antibodies  >629 publications with CA2 and Fibrosis

Organs/Tissues associated with anti-CA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >20669 publications with CA2 and Brain
 Blood Antibodies  >17604 publications with CA2 and Blood
 Heart Antibodies  >14833 publications with CA2 and Heart
 Liver Antibodies  >4970 publications with CA2 and Liver
 Kidney Antibodies  >4529 publications with CA2 and Kidney
 Pancreas Antibodies  >3273 publications with CA2 and Pancreas
 Intestine Antibodies  >2526 publications with CA2 and Intestine
 Eye Antibodies  >2510 publications with CA2 and Eye
 Lung Antibodies  >2117 publications with CA2 and Lung
 Embryonic Tissue Antibodies  >1754 publications with CA2 and Embryonic Tissue
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