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CPB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178497

Rabbit IgG polyclonal antibody for Carboxypeptidase B2 (CPB2) detection. Background: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that,… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CPB2, Polyclonal Antibody
Full Product Name
Anti-CPB2 Antibody
Product Gene Name
anti-CPB2 antibody
Product Synonym Names
Carboxypeptidase U; CarboxypeptidaseU; CarboxypeptidaseB2; Carboxypeptidase B2; CP B2; CPB2; CPB 2; CPU; PCPB; Procarboxypeptidase B; Procarboxypeptidase R; Procarboxypeptidase U; TAFI; Q96IY4; Carboxypeptidase B2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
40,922 Da
OMIM
603101
3D Structure
ModBase 3D Structure for Q96IY4
Immunogen
E Coli-derived human CPB2 recombinant protein (Position: K166-D388). Human CPB2 shares 86.1% and 85.7% amino acid (aa) sequence identity with mouse and rat CPB2, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CPB2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-CPB2 antibody
Western Blot: 0.1-0.5mug/ml; Tested Species: Human
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
ELISA: 0.1-0.5mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western Blot (WB) of anti-CPB2 antibody

Western blot analysis of CPB2 expression in human placenta extract (lane 1) and HEPG2 whole cell lysates (lane 2). CPB2 at 48KD, 60KD was detected using rabbit anti- CPB2 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-CPB2 Polyclonal Antibody – Cat# MBS178497

Immunohistochemistry (IHC) of anti-CPB2 antibody

CPB2 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- CPB2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-CPB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178497

Product References and Citations for anti-CPB2 antibody

1. Boffa MB, Reid TS, Joo E, Nesheim ME, Koschinsky ML (May 1999). "Characterization of the gene encoding human TAFI (thrombin-activable fibrinolysis inhibitor; plasma procarboxypeptidase B)". Biochemistry 38 (20): 6547-58.
2. Eaton DL, Malloy BE, Tsai SP, Henzel W, Drayna D (Dec 1991). "Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma". J Biol Chem 266 (32): 21833-8.
3. Tsai SP, Drayna D (Dec 1992). "The gene encoding human plasma carboxypeptidase B (CPB2) resides on chromosome 13". Genomics14 (2): 549-50.

Other Notes

Small volumes of anti-CPB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CPB2 purchase

MBS178497 is an antibody targeting CPB2. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CPB2. It may not necessarily be applicable to this product.

NCBI GI #
513126877
NCBI GeneID
1361
NCBI Accession #
NP_001265470.1
UniProt Primary Accession #
Q96IY4
UniProt Secondary Accession #
Q15114; Q5T9K1; Q5T9K2; Q9P2Y6; A8K464
UniProt Related Accession #
Q96IY4
GenBank Nucleotide #
NM_001278541.1
NCBI Official Full Name
carboxypeptidase B2 isoform 2 preproprotein
NCBI Official Synonym Full Names
carboxypeptidase B2
NCBI Official Symbol
CPB2
NCBI Official Synonym Symbols
CPU; PCPB; TAFI
UniProt Protein Name
Carboxypeptidase B2
UniProt Synonym Protein Names
Carboxypeptidase U; CPU; Plasma carboxypeptidase B; pCPB; Thrombin-activable fibrinolysis inhibitor; TAFI
Protein Family
Carboxypeptidase
UniProt Gene Name
CPB2
UniProt Synonym Gene Names
CPU; pCPB; TAFI
UniProt Entry Name
CBPB2_HUMAN
NCBI Protein Information
carboxypeptidase B2

NCBI Summary for CPB2

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
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UniProt Comments for CPB2

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; EC 3.4.17.20; Secreted

Chromosomal Location of Human Ortholog: 13q14.11

Biological Process: fibrinolysis
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Research Articles on CPB2

1. Pro-inflammatory cytokines or lipopolysaccharide decreased TAFI protein levels via binding of tristetraprolin to the CPB2 3'-UTR, which mediates CPB2 mRNA destabilisation. HuR can bind the CBP2 3'UTR. This is reduced by pro-inflammatory mediators.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CPB2 antibody

 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram

Diseases associated with anti-CPB2 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >17 publications with CPB2 and Inflammation
 Cardiovascular Diseases Antibodies  >13 publications with CPB2 and Cardiovascular Diseases
 Necrosis Antibodies  >13 publications with CPB2 and Necrosis
 Vascular Diseases Antibodies  >10 publications with CPB2 and Vascular Diseases
 Liver Diseases Antibodies  >5 publications with CPB2 and Liver Diseases
 Carcinoma Antibodies  >4 publications with CPB2 and Carcinoma
 Edema Antibodies  >3 publications with CPB2 and Edema
 Hepatitis Antibodies  >3 publications with CPB2 and Hepatitis
 Carcinoma, Hepatocellular Antibodies  >2 publications with CPB2 and Carcinoma, Hepatocellular
 Atherosclerosis Antibodies  >2 publications with CPB2 and Atherosclerosis

Organs/Tissues associated with anti-CPB2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >15 publications with CPB2 and Liver
 Brain Antibodies  >5 publications with CPB2 and Brain
 Muscle Antibodies  >4 publications with CPB2 and Muscle
 Embryonic Tissue Antibodies  >1 publications with CPB2 and Embryonic Tissue
 Spleen Antibodies  >1 publications with CPB2 and Spleen
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