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Visfatin (NAMPT) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178509

Rabbit IgG polyclonal antibody for Nicotinamide phosphoribosyltransferase (NAMPT) detection. Background: Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in… More details

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Product Overview

Online Datasheet in PDF
Product Name
Visfatin (NAMPT), Polyclonal Antibody
Full Product Name
Anti-Visfatin Antibody
Product Gene Name
anti-NAMPT antibody
Product Synonym Names
NAmPRTase; Nampt; PBEF 1; PBEF1; PBEF; VF; Visfatin; P43490; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
55,521 Da
OMIM
608764
3D Structure
ModBase 3D Structure for P43490
Immunogen
E Coli-derived human Visfatin recombinant protein (Position: L62-N337). Human Visfatin shares 97.1% and 96.7% amino acid (aa) sequence identity with mouse and rat Visfatin, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-NAMPT antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-NAMPT antibody
Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
ELISA: 0.1-0.5mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.

Western Blot (WB) of anti-NAMPT antibody

Western blot analysis of Visfatin expression in rat brain extract (lane 1), mouse cardiac muscle extract (lane 2), and human placenta extract (lane 3). Visfatin at 56KD was detected using rabbit anti-Visfatin Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-NAMPT Polyclonal Antibody – Cat# MBS178509

Immunohistochemistry (IHC) of anti-NAMPT antibody

Visfatin was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- Visfatin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-NAMPT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178509

Immunohistochemistry (IHC) of anti-NAMPT antibody

Visfatin was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Visfatin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-NAMPT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178509

Immunohistochemistry (IHC) of anti-NAMPT antibody

Visfatin was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- Visfatin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-NAMPT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178509

Product References and Citations for anti-NAMPT antibody

1. "Entrez Gene: PBEF1 pre-B-cell colony enhancing factor 1".
2. Samal B, Sun Y, Stearns G, Xie C, Suggs S, McNiece I (February 1994). "Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor". Mol. Cell. Biol. 14 (2): 1431-7.
3. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C (July 2006). "Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme". Nat. Struct. Mol. Biol. 13 (7): 661-2.

Other Notes

Small volumes of anti-NAMPT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAMPT purchase

MBS178509 is an antibody targeting NAMPT. Also known as Anti-Visfatin Antibody. Reactive in Human, Mouse, Rat
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NAMPT. It may not necessarily be applicable to this product.

NCBI GI #
5031977
NCBI GeneID
10135
NCBI Accession #
NP_005737.1
UniProt Primary Accession #
P43490
UniProt Secondary Accession #
Q3KQV0; Q8WW95; A4D0Q9; A4D0R0
UniProt Related Accession #
P43490
GenBank Nucleotide #
NM_005746.2
NCBI Official Full Name
nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Synonym Protein Names
Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin
Protein Family
Nicotinamide phosphoribosyltransferase
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
UniProt Entry Name
NAMPT_HUMAN
NCBI Protein Information
nicotinamide phosphoribosyltransferase

NCBI Summary for NAMPT

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]
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UniProt Comments for NAMPT

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cytokine; Transferase; Cell cycle regulation; EC 2.4.2.12

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: cell junction; cytosol; nucleoplasm

Molecular Function: nicotinamide phosphoribosyltransferase activity; protein binding

Biological Process: cell-cell signaling; circadian regulation of gene expression; circadian rhythm; insulin receptor signaling pathway; nicotinamide metabolic process; positive regulation of cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; signal transduction
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Research Articles on NAMPT

1. Visfatin levels might be a useful biomarker for predicting CSF in patients undergoing diagnostic coronary angiography.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NAMPT antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 NAD Salvage Pathway antibodies  NAD Salvage Pathway Diagram
 Nicotinamide Salvaging Pathway antibodies  Nicotinamide Salvaging Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram

Diseases associated with anti-NAMPT antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >97 publications with NAMPT and Inflammation
 Obesity Antibodies  >79 publications with NAMPT and Obesity
 Disease Models, Animal Antibodies  >28 publications with NAMPT and Disease Models, Animal
 Nervous System Diseases Antibodies  >25 publications with NAMPT and Nervous System Diseases
 Necrosis Antibodies  >24 publications with NAMPT and Necrosis
 Liver Diseases Antibodies  >23 publications with NAMPT and Liver Diseases
 Heart Diseases Antibodies  >18 publications with NAMPT and Heart Diseases
 Fatty Liver Antibodies  >17 publications with NAMPT and Fatty Liver
 Neoplasms, Experimental Antibodies  >11 publications with NAMPT and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >11 publications with NAMPT and Breast Neoplasms

Organs/Tissues associated with anti-NAMPT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >117 publications with NAMPT and Blood
 Adipose Tissue Antibodies  >53 publications with NAMPT and Adipose Tissue
 Liver Antibodies  >46 publications with NAMPT and Liver
 Muscle Antibodies  >45 publications with NAMPT and Muscle
 Vascular Antibodies  >40 publications with NAMPT and Vascular
 Brain Antibodies  >38 publications with NAMPT and Brain
 Heart Antibodies  >29 publications with NAMPT and Heart
 Connective Tissue Antibodies  >27 publications with NAMPT and Connective Tissue
 Lung Antibodies  >18 publications with NAMPT and Lung
 Kidney Antibodies  >16 publications with NAMPT and Kidney
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