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Mast Cell Tryptase (TPSAB1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178516

Background: Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as… More details

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Product Overview

Online Datasheet in PDF
Product Name
Mast Cell Tryptase (TPSAB1), Polyclonal Antibody
Full Product Name
Anti-Mast Cell Tryptase Antibody
Product Gene Name
anti-TPSAB1 antibody
Product Synonym Names
alpha II; Lung tryptase; MCP 7; Pituitary tryptase; Skin tryptase; TPS 1; TPS1; TPS2; TPSAB1; TPSAB1 protein; TPSB1; Tryptase 1; Tryptase alpha 1; tryptase alpha I included; Tryptase alpha II; tryptase alpha II included; tryptase alpha included; tryptase alpha/beta 1; Tryptase beta 1; tryptase beta I included; Tryptase I; tryptase I included; Tryptase III; Tryptase skin; Q15661; Tryptase alpha/beta-1; tryptase alpha/beta 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized ; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
191080
3D Structure
ModBase 3D Structure for Q15661
Immunogen
E Coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275). Human Mast Cell Tryptase shares 77% and 76.1% amino acid (aa) sequence identity with mouse and rat Mast Cell Tryptase, respectively.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
Lab provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescent kit (Please inquire) in Western Blot (WB), supporeted by (catalog : please inquire) in IHC-P .
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TPSAB1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-TPSAB1 antibody
Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Human
Immunohistochemisry Paraffin: Concentration: 0.5-1ug/ml; Tested Species: Human; Antigen Retrieval: By Heat
ELISA: Concentration: 0.1-0.5ug/ml; Tested Species: Human

Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-TPSAB1 antibody

Western blot analysis of Mast Cell Tryptase expression in 293T whole cell lysates (lane 1). Mast Cell Tryptase at 30KD was detected using rabbit anti- Mast Cell Tryptase Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-TPSAB1 Polyclonal Antibody – Cat# MBS178516

Immunohistochemistry (IHC) of anti-TPSAB1 antibody

Mast Cell Tryptase was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Mast Cell Tryptase Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-TPSAB1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178516

Product References and Citations for anti-TPSAB1 antibody

1. "Entrez Gene: TPSAB1 tryptase alpha/beta 1".
2. Miller JS, Moxley G, Schwartz LB (Oct 1990). "Cloning and characterization of a second complementary DNA for human tryptase". J Clin Invest 86 (3): 864-70.
3. Pallaoro M, Fejzo MS, Shayesteh L, Blount JL, Caughey GH (Feb 1999). "Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3". J Biol Chem 274 (6): 3355-62.

Other Notes

Small volumes of anti-TPSAB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPSAB1 purchase

MBS178516 is an antibody targeting TPSAB1. Also known as Anti-Mast Cell Tryptase Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TPSAB1. It may not necessarily be applicable to this product.

NCBI GI #
13775595
NCBI GeneID
7177
NCBI Accession #
NP_003285.2
UniProt Primary Accession #
Q15661
UniProt Secondary Accession #
P15157; Q15663; Q6B052; Q9H2Y4; Q9H2Y5; Q9UQI1; D2E6R9; D2E6S1
UniProt Related Accession #
Q15661
GenBank Nucleotide #
NM_003294.3
NCBI Official Full Name
tryptase alpha/beta-1
NCBI Official Synonym Full Names
tryptase alpha/beta 1
NCBI Official Symbol
TPSAB1
NCBI Official Synonym Symbols
TPS1; TPS2; TPSB1
UniProt Protein Name
Tryptase alpha/beta-1
UniProt Synonym Protein Names
Tryptase I; Tryptase alpha-1
Protein Family
Tryptase
UniProt Gene Name
TPSAB1
UniProt Synonym Gene Names
TPS1; TPS2; TPSB1; Tryptase-1
UniProt Entry Name
TRYB1_HUMAN
NCBI Protein Information
tryptase alpha/beta-1

NCBI Summary for TPSAB1

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
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UniProt Comments for TPSAB1

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted, signal peptide; EC 3.4.21.59; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: defense response; extracellular matrix disassembly
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Research Articles on TPSAB1

1. We found the determination of serum tryptase levels over time to be a reassuring correlate of clinical improvement in pediatric mastocytosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TPSAB1 antibody

 Reference Product  PubMed Publications
 CPA3 antibody  >3 publications with TPSAB1 and CPA3
 RAC1 antibody  >1 publications with TPSAB1 and RAC1
 CTSC antibody  >1 publications with TPSAB1 and CTSC
 RAC2 antibody  >1 publications with TPSAB1 and RAC2
 LGALS3 antibody  >1 publications with TPSAB1 and LGALS3

Pathways associated with anti-TPSAB1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram

Diseases associated with anti-TPSAB1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >29 publications with TPSAB1 and Inflammation
 Lung Diseases Antibodies  >9 publications with TPSAB1 and Lung Diseases
 Fibrosis Antibodies  >7 publications with TPSAB1 and Fibrosis
 Necrosis Antibodies  >5 publications with TPSAB1 and Necrosis
 Hyperplasia Antibodies  >4 publications with TPSAB1 and Hyperplasia
 Leukemia Antibodies  >4 publications with TPSAB1 and Leukemia
 Edema Antibodies  >3 publications with TPSAB1 and Edema
 Fever Antibodies  >1 publications with TPSAB1 and Fever
 Proteinuria Antibodies  >1 publications with TPSAB1 and Proteinuria
 Kidney Diseases Antibodies  >1 publications with TPSAB1 and Kidney Diseases

Organs/Tissues associated with anti-TPSAB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >24 publications with TPSAB1 and Skin
 Bone Antibodies  >21 publications with TPSAB1 and Bone
 Lung Antibodies  >21 publications with TPSAB1 and Lung
 Bone Marrow Antibodies  >20 publications with TPSAB1 and Bone Marrow
 Connective Tissue Antibodies  >16 publications with TPSAB1 and Connective Tissue
 Intestine Antibodies  >9 publications with TPSAB1 and Intestine
 Nerve Antibodies  >6 publications with TPSAB1 and Nerve
 Stomach Antibodies  >3 publications with TPSAB1 and Stomach
 Kidney Antibodies  >3 publications with TPSAB1 and Kidney
 Testis Antibodies  >2 publications with TPSAB1 and Testis
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