SFRP5 ELISA Kit for Human

Principle of the Assay: Human SFRP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SFRP5 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: E30-H317) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SFRP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SFRP5 amount of sample captured in plate.

Background/Introduction: Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wntbinding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Small volumes of SFRP5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178536 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SFRP5, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SFRP5. The ELISA analytical biochemical technique of the MBS178536 kit is based on SFRP5 antibody-SFRP5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SFRP5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SFRP5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).