R-Spondin-4 (RSPO4) ELISA Kit for Human

Principle of the Assay: Human R-Spondin-4 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for R-Spondin-4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: L20-P234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for R-Spondin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human R-Spondin-4 amount of sample captured in plate.

Background/Introduction: R-spondin 4 is a protein in humans that is encoded by the RSPO4 gene. It is mapped to chromosome 20p13. This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.

Small volumes of RSPO4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178541 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the R-Spondin-4 (RSPO4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RSPO4. The ELISA analytical biochemical technique of the MBS178541 kit is based on RSPO4 antibody-RSPO4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSPO4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSPO4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).