Siglec-7/CD328 (SIGLEC7) ELISA Kit for Human

Principle of the Assay: Human Siglec-7/CD328 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for Siglec-7/CD328 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q19-L353) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Siglec-7/CD328 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Siglec-7/CD328 amount of sample captured in plate.

Background/Introduction: Sialic acid-binding Ig-like lectin 7, also known as CD328 (cluster of differentiation 328) or AIRM1, is a protein that in humans is encoded by the SIGLEC7 gene. And this gene is mapped to chromosome 19. AIRM1 is a novel member of the sialoadhesin family characterized by 3 immunoglobulin-like extracellular domains (1 N-terminal V type and 2 C2 type) and a classic immunoreceptor tyrosinebased inhibitory motif (ITIM) in the cytoplasmic portion. The highest amino acid sequence similarity found was with the myeloid-specific CD33 molecule, and similar to other sialoadhesin molecules, AIRM1 appears to mediate sialic acid-dependent ligand recognition.

Small volumes of SIGLEC7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178551 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Siglec-7/CD328 (SIGLEC7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SIGLEC7. The ELISA analytical biochemical technique of the MBS178551 kit is based on SIGLEC7 antibody-SIGLEC7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIGLEC7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIGLEC7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).