R-Spondin-3 (RSPO3) ELISA Kit for Mouse

Principle of the Assay: Mouse R-Spondin-3 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A specific for R-Spondin-3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q22-H277) and test samples are added to the wells, a biotinylated detection specific for R-Spondin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse R-Spondin-3 amount of sample captured in plate.

Background: R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the Rspondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.

Small volumes of RSPO3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178563 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the R-Spondin-3 (RSPO3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RSPO3. The ELISA analytical biochemical technique of the MBS178563 kit is based on RSPO3 antibody-RSPO3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSPO3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSPO3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).