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TNFRSF10B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178610

Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10B(TNFRSF10B) detection.Background: TNFRSF10B(Tumor necrosis factor receptor superfamily, member 10b) is a human gene. It is also known as DR5, CD262, KILLER, TRICK2,… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DR5 (TNFRSF10B), Polyclonal Antibody
Full Product Name
Anti-DR5 Antibody
Product Gene Name
anti-TNFRSF10B antibody
Product Synonym Names
CD262 antigen; Cytotoxic TRAIL receptor 2; DR5; Dr5; Killer; KILLER; O14763; TNFRSF10B; TR10B; trail r2; trailr 2; TRAILR2; TRAIL-R2; TRICK2; TRICK2A; TRICK2B; Tumor necrosis factor receptor superfamily member 10B
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
45,083 Da
OMIM
AF018657 mRNA
3D Structure
ModBase 3D Structure for O14763
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E Coli-derived human DR5 recombinant protein (Position: K233-S440). Human DR5 shares 48.4% amino acid (aa) sequence identity with mouse DR5.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TNFRSF10B antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-TNFRSF10B antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-TNFRSF10B antibody

Western blot analysis of DR5 expression in rat thymus extract (lane 1), mouse cardiac muscle extract (lane 2) and K562 whole cell lysates (lane 3). DR5 at 60KD was detected using rabbit anti- DR5 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-TNFRSF10B Polyclonal Antibody – Cat# MBS178610

Immunohistochemistry (IHC) of anti-TNFRSF10B antibody

DR5 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- DR5 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-TNFRSF10B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178610

Immunohistochemistry (IHC) of anti-TNFRSF10B antibody

DR5 was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- DR5 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-TNFRSF10B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178610

Immunohistochemistry (IHC) of anti-TNFRSF10B antibody

DR5 was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- DR5 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-TNFRSF10B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178610

Product References and Citations for anti-TNFRSF10B antibody

1. Finnberg, N., Klein-Szanto, A. J. P., El-Deiry, W. S.TRAIL-R deficiency in mice promotes susceptibility to chronic inflammation and tumorigenesis.J. Clin. Invest. 118: 111-123, 2008.
2. Takeda, K., Kojima, Y., Ikejima, K., Harada, K., Yamashina, S., Okumura, K., Aoyama, T., Frese, S., Ikeda, H., Haynes, N. M., Cretney, E., Yagita, H., Sueyoshi, N., Sato, N., Nakanuma, Y., Smyth, M. J., Okumura, K.Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease.Proc. Nat. Acad. Sci. 105: 10895-10900, 2008.
3. Walczak, H., Degli-Esposti, M. A., Johnson, R. S., Smolak, P. J., Waugh, J. Y., Boiani, N., Timour, M. S., Gerhart, M. J., Schooley, K. A., Smith, C. A., Goodwin, R. G., Rauch, C. T.TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL.EMBO J. 16: 5386-5397, 1997.

Other Notes

Small volumes of anti-TNFRSF10B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNFRSF10B purchase

MBS178610 is an antibody targeting TNFRSF10B. Also known as Anti-DR5 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFRSF10B. It may not necessarily be applicable to this product.

NCBI GI #
224494019
NCBI GeneID
8795
NCBI Accession #
NP_003833.4
UniProt Primary Accession #
O14763
UniProt Secondary Accession #
O14720; O15508; O15517; O15531; Q6UXM8; Q7Z360; Q9BVE0
UniProt Related Accession #
O14763
GenBank Nucleotide #
NM_003842.4
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 10B isoform 1
NCBI Official Synonym Full Names
TNF receptor superfamily member 10b
NCBI Official Symbol
TNFRSF10B
NCBI Official Synonym Symbols
DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 10B
UniProt Synonym Protein Names
Death receptor 5; TNF-related apoptosis-inducing ligand receptor 2; TRAIL receptor 2; TRAIL-R2; CD_antigen: CD262
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
TNFRSF10B
UniProt Synonym Gene Names
DR5; KILLER; TRAILR2; TRICK2; ZTNFR9; TRAIL receptor 2; TRAIL-R2
NCBI Protein Information
tumor necrosis factor receptor superfamily member 10B

NCBI Summary for TNFRSF10B

The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]
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UniProt Comments for TNFRSF10B

TRAIL-R2: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. Homotrimer. Can interact with TRADD and RIPK1. Regulated by p53/TP53. Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: cell surface; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity

Biological Process: caspase activation; cell surface receptor linked signal transduction; immune response; induction of apoptosis via death domain receptors; inflammatory response; multicellular organismal development; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide

Disease: Squamous Cell Carcinoma, Head And Neck
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Research Articles on TNFRSF10B

1. CAPE/TRAIL stimulated apoptosis through the binding of TRAIL to DR5. Moreover, expression of transcription factor C/EBP homologous protein (CHOP) markedly increased in response to CAPE and transient knockdown of CHOP abolished CAPE/TRAIL-mediated apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNFRSF10B antibody

 Reference Product  PubMed Publications
 TNFSF10 antibody  >346 publications with TNFRSF10B and TNFSF10
 TNFRSF10A antibody  >308 publications with TNFRSF10B and TNFRSF10A
 CASP8 antibody  >195 publications with TNFRSF10B and CASP8
 FADD antibody  >107 publications with TNFRSF10B and FADD
 CFLAR antibody  >50 publications with TNFRSF10B and CFLAR
 TRADD antibody  >17 publications with TNFRSF10B and TRADD
 TP53 antibody  >12 publications with TNFRSF10B and TP53
 RIPK1 antibody  >10 publications with TNFRSF10B and RIPK1
 TRAF2 antibody  >6 publications with TNFRSF10B and TRAF2
 CASP10 antibody  >4 publications with TNFRSF10B and CASP10

Pathways associated with anti-TNFRSF10B antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies  Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
 CASP8 Activity Is Inhibited Pathway antibodies  CASP8 Activity Is Inhibited Pathway Diagram
 Caspase Activation Via Extrinsic Apoptotic Signalling Pathway antibodies  Caspase Activation Via Extrinsic Apoptotic Signalling Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with anti-TNFRSF10B antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >556 publications with TNFRSF10B and Necrosis
 Lung Diseases Antibodies  >40 publications with TNFRSF10B and Lung Diseases
 Lung Neoplasms Antibodies  >39 publications with TNFRSF10B and Lung Neoplasms
 Breast Neoplasms Antibodies  >39 publications with TNFRSF10B and Breast Neoplasms
 Liver Diseases Antibodies  >38 publications with TNFRSF10B and Liver Diseases
 Colonic Neoplasms Antibodies  >36 publications with TNFRSF10B and Colonic Neoplasms
 Nervous System Diseases Antibodies  >26 publications with TNFRSF10B and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >25 publications with TNFRSF10B and Neoplasms, Experimental
 Inflammation Antibodies  >23 publications with TNFRSF10B and Inflammation
 Carcinoma, Hepatocellular Antibodies  >23 publications with TNFRSF10B and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-TNFRSF10B antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >109 publications with TNFRSF10B and Blood
 Liver Antibodies  >56 publications with TNFRSF10B and Liver
 Lung Antibodies  >55 publications with TNFRSF10B and Lung
 Prostate Antibodies  >28 publications with TNFRSF10B and Prostate
 Brain Antibodies  >26 publications with TNFRSF10B and Brain
 Kidney Antibodies  >18 publications with TNFRSF10B and Kidney
 Bone Marrow Antibodies  >14 publications with TNFRSF10B and Bone Marrow
 Skin Antibodies  >12 publications with TNFRSF10B and Skin
 Muscle Antibodies  >12 publications with TNFRSF10B and Muscle
 Vascular Antibodies  >11 publications with TNFRSF10B and Vascular
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