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Cytokeratin 14 (KRT14) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178647

Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 14(KRT14) detection.Background: Cytokeratin 14, also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14), is a member of the type I keratin family of intermediate filament proteins. In humans… More details

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Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 14 (KRT14), Polyclonal Antibody
Full Product Name
Anti-Cytokeratin 14 Antibody
Product Gene Name
anti-KRT14 antibody
Product Synonym Names
CK14; CK-14; CK 14; Cytokeratin14; Cytokeratin-14; Cytokeratin 14; Dowling Meara; EBS3; EBS4; K14; Keratin 14; Keratin; Keratin-14; Keratin14; Koebner; Krt 14; Krt14; NFJ; P02533; Keratin, type I cytoskeletal 14
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
51,561 Da
OMIM
J00124 Genomic DNA
3D Structure
ModBase 3D Structure for P02533
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 14 (446-472aa RQIRTKVMDVHDGKVVSTHEQVLRTKN), identical to the related mouse and rat sequences.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-KRT14 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-KRT14 antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-KRT14 antibody

Western blot analysis of Cytokeratin 14 expression in rat brain extract (lane 1), NIH3T3 whole cell lysates (lane 2) and HEPG2 whole cell lysates (lane 3). Cytokeratin 14 at 60KD was detected using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-KRT14 Polyclonal Antibody – Cat# MBS178647

Immunohistochemistry (IHC) of anti-KRT14 antibody

Cytokeratin 14 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-KRT14 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178647

Immunohistochemistry (IHC) of anti-KRT14 antibody

Cytokeratin 14 was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-KRT14 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178647

Immunohistochemistry (IHC) of anti-KRT14 antibody

Cytokeratin 14 was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-KRT14 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178647

Product References and Citations for anti-KRT14 antibody

1. Batta, K., Rugg, E. L., Wilson, N. J., West, N., Goodyear, H., Lane, E. B., Gratian, M., Dopping-Hepenstal, P., Moss, C., Eady, R. A. J. A keratin 14 'knockout' mutation in recessive epidermolysis bullosa simplex resulting in less severe disease. Brit. J. Derm. 143: 621-627, 2000.
2. Chan, Y., Anton-Lamprecht, I., Yu, Q.-C., Jackel, A., Zabel, B., Ernst, J.-P., Fuchs, E. A human keratin 14 'knockout': the absence of K14 leads to severe epidermolysis bullosa simplex and a function for an intermediate filament protein. Genes Dev. 8: 2574-2587, 1994.
3. Chen, H., Bonifas, J. M., Mstsumura, K., Ikeda, S., Leyden, W. A., Epstein, E. H. Keratin 14 gene mutations in patients with epidermolysis bullosa simplex. J. Invest. Derm. 105: 629-632, 1995.

Other Notes

Small volumes of anti-KRT14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT14 purchase

MBS178647 is an antibody targeting KRT14. Also known as Anti-Cytokeratin 14 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT14. It may not necessarily be applicable to this product.

NCBI GI #
15431310
NCBI GeneID
3861
NCBI Accession #
NP_000517.2
UniProt Primary Accession #
P02533
UniProt Secondary Accession #
Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4
UniProt Related Accession #
P02533
GenBank Nucleotide #
NM_000526.4
NCBI Official Full Name
keratin, type I cytoskeletal 14
NCBI Official Synonym Full Names
keratin 14
NCBI Official Symbol
KRT14
NCBI Official Synonym Symbols
K14; NFJ; CK14; EBS3; EBS4
UniProt Protein Name
Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names
Cytokeratin-14; CK-14; Keratin-14; K14
Protein Family
Keratin
UniProt Gene Name
KRT14
UniProt Synonym Gene Names
CK-14; K14
NCBI Protein Information
keratin, type I cytoskeletal 14

NCBI Summary for KRT14

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT14

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; cytosol; intermediate filament; keratin filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: aging; epidermis development; hair cycle; hemidesmosome assembly; intermediate filament bundle assembly

Disease: Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex, Dowling-meara Type; Epidermolysis Bullosa Simplex, Generalized; Epidermolysis Bullosa Simplex, Localized; Naegeli Syndrome
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Research Articles on KRT14

1. identification of 29 different mutations in KRT5 and KRT14, 11 of which were novel, in a Polish cohort of epidermolysis bullosa simplex patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT14 antibody

 Reference Product  PubMed Publications
 KRT5 antibody  >109 publications with KRT14 and KRT5
 DST antibody  >4 publications with KRT14 and DST
 IVL antibody  >1 publications with KRT14 and IVL
 CREBBP antibody  >1 publications with KRT14 and CREBBP
 KRT3 antibody  >1 publications with KRT14 and KRT3

Pathways associated with anti-KRT14 antibody

 Products by Pathway  Pathway Diagram
 Corticotropin-releasing Hormone Signaling Pathway antibodies  Corticotropin-releasing Hormone Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Keratinization Pathway antibodies  Keratinization Pathway Diagram

Diseases associated with anti-KRT14 antibody

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >106 publications with KRT14 and Congenital Abnormalities
 Epidermolysis Bullosa Simplex Antibodies  >103 publications with KRT14 and Epidermolysis Bullosa Simplex
 Carcinoma Antibodies  >62 publications with KRT14 and Carcinoma
 Carcinoma, Squamous Cell Antibodies  >39 publications with KRT14 and Carcinoma, Squamous Cell
 Hyperplasia Antibodies  >21 publications with KRT14 and Hyperplasia
 Breast Neoplasms Antibodies  >20 publications with KRT14 and Breast Neoplasms
 Inflammation Antibodies  >15 publications with KRT14 and Inflammation
 Necrosis Antibodies  >13 publications with KRT14 and Necrosis
 Cell Transformation, Neoplastic Antibodies  >13 publications with KRT14 and Cell Transformation, Neoplastic
 Pigmentation Disorders Antibodies  >10 publications with KRT14 and Pigmentation Disorders

Organs/Tissues associated with anti-KRT14 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >177 publications with KRT14 and Skin
 Blood Antibodies  >25 publications with KRT14 and Blood
 Mouth Antibodies  >16 publications with KRT14 and Mouth
 Lung Antibodies  >14 publications with KRT14 and Lung
 Eye Antibodies  >10 publications with KRT14 and Eye
 Bladder Antibodies  >10 publications with KRT14 and Bladder
 Mammary Gland Antibodies  >8 publications with KRT14 and Mammary Gland
 Esophagus Antibodies  >8 publications with KRT14 and Esophagus
 Liver Antibodies  >7 publications with KRT14 and Liver
 Connective Tissue Antibodies  >6 publications with KRT14 and Connective Tissue
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