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CPM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178652

Rabbit IgG polyclonal antibody for Carboxypeptidase M(CPM) detection.Background: CPM, mapped to 12q15, is known as Carboxypeptidase M. The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CPM, Polyclonal Antibody
Full Product Name
Anti-CPM Antibody
Product Gene Name
Product Synonym Names
Carboxypeptidase M; CPM; Renal carboxypeptidase; Urinary carboxypeptidase B; P14384
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
50,514 Da
OMIM
J04970 mRNA
3D Structure
ModBase 3D Structure for P14384
Notes
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CPM (286-316aa KYPREEKLPSFWNNNKASLIEYIKQVHLGVK), different from the related mouse sequence by two amino acids.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CPM antibody
Western Blot (WB)
Application Notes for anti-CPM antibody
Western Blot: 0.1-0.5ug/ml

Western Blot (WB) of anti-CPM antibody

Western blot analysis of CPM expression in HEPG2 whole cell lysates (lane 1). CPM at 65KD was detected using rabbit anti- CPM Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-CPM Polyclonal Antibody – Cat# MBS178652

Product References and Citations for anti-CPM antibody

1. Kas, K., Schoenmakers, E. F. P. M., Van de Ven, W. J. M. Physical map location of the human carboxypeptidase M gene (CPM) distal to D12S375 and proximal to D12S8 at chromosome 12q15. Genomics 30: 403-405, 1995.
2. Rehli, M., Krause, S. W., Kreutz, M., Andreesen, R. Carboxypeptidase M is identical to the MAX.1 antigen and its expression is associated with monocyte to macrophage differentiation. J. Biol. Chem. 270: 15644-15649, 1995.
3. Tan, F., Chan, S. J., Steiner, D. F., Schilling, J. W., Skidgel, R. A. Molecular cloning and sequencing of the cDNA for human membrane-bound carboxypeptidase M: comparison with carboxypeptidases A, B, H, and N. J. Biol. Chem. 264: 13165-13170, 1989.

Other Notes

Small volumes of anti-CPM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CPM purchase

MBS178652 is an antibody targeting CPM. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CPM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carboxypeptidase M
NCBI Official Synonym Full Names
carboxypeptidase M
NCBI Official Symbol
CPM
UniProt Protein Name
Carboxypeptidase M
Protein Family
UniProt Gene Name
CPM
UniProt Synonym Gene Names
CPM
NCBI Protein Information
carboxypeptidase M

NCBI Summary for CPM

The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CPM

CPM: Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. Belongs to the peptidase M14 family.

Protein type: EC 3.4.17.12; Membrane protein, GPI anchor; Protease

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: cell surface; extracellular space

Molecular Function: carboxypeptidase activity; serine carboxypeptidase activity

Biological Process: anatomical structure morphogenesis; peptide metabolic process; protein processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CPM antibody

 Reference Product  PubMed Publications
 EGF antibody  >22 publications with CPM and EGF
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