• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Islet 1 (ISL1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178676

Rabbit IgG polyclonal antibody for Insulin gene enhancer protein ISL-1(ISL1) detection.Background: Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Islet 1 (ISL1), Polyclonal Antibody
Full Product Name
Anti-Islet 1 Antibody
Product Gene Name
anti-ISL1 antibody
Product Synonym Names
ISL 1; ISL1; Islet-1; Islet1; ISLET1; P61371; Insulin gene enhancer protein ISL-1; ISL LIM homeobox 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
39,036 Da
OMIM
S70721 mRNA
3D Structure
ModBase 3D Structure for P61371
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Islet 1 (118-161aa DEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAE), identical to the related mouse and rat sequences.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ISL1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-ISL1 antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-ISL1 antibody

Western blot analysis of Islet 1 expression in PC12 whole cell lysates (lane 1) and MCF-7 whole cell lysates (lane 2). Islet 1 at 39KD was detected using rabbit anti- Islet 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-ISL1 Polyclonal Antibody – Cat# MBS178676

Immunohistochemistry (IHC) of anti-ISL1 antibody

Islet 1 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Islet 1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-ISL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178676

Product References and Citations for anti-ISL1 antibody

1. "Entrez Gene: ISL1 ISL1 transcription factor, LIM/homeodomain, (islet-1)".
2. Gay F, Anglade I, Gong Z, Salbert G (October 2000). "The LIM/homeodomain protein islet-1 modulates estrogen receptor functions". Mol. Endocrinol. 14 (10): 1627-48.
3. Tanizawa Y, Riggs AC, Dagogo-Jack S, Vaxillaire M, Froguel P, Liu L, Donis-Keller H, Permutt MA (July 1994). "Isolation of the human LIM/homeodomain gene islet-1 and identification of a simple sequence repeat polymorphism [corrected]". Diabetes. 43 (7): 935-41.

Other Notes

Small volumes of anti-ISL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ISL1 purchase

MBS178676 is an antibody targeting ISL1. Also known as Anti-Islet 1 Antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ISL1. It may not necessarily be applicable to this product.

NCBI GI #
115387114
NCBI GeneID
3670
NCBI Accession #
NP_002193.2
UniProt Primary Accession #
P61371
UniProt Secondary Accession #
P20663; P47894
UniProt Related Accession #
P61371
GenBank Nucleotide #
NM_002202.2
NCBI Official Full Name
insulin gene enhancer protein ISL-1
NCBI Official Synonym Full Names
ISL LIM homeobox 1
NCBI Official Symbol
ISL1
NCBI Official Synonym Symbols
Isl-1; ISLET1
UniProt Protein Name
Insulin gene enhancer protein ISL-1
Protein Family
Insulin gene enhancer protein
UniProt Gene Name
ISL1
UniProt Synonym Gene Names
Islet-1
NCBI Protein Information
insulin gene enhancer protein ISL-1

NCBI Summary for ISL1

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for ISL1

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Cell development/differentiation; DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: nucleoplasm

Molecular Function: bHLH transcription factor binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; protein binding

Biological Process: mesenchymal cell differentiation; negative regulation of estrogen receptor signaling pathway; negative regulation of inflammatory response; negative regulation of neuron apoptosis; negative regulation of transcription from RNA polymerase II promoter; neuron fate specification; pancreas development; peripheral nervous system neuron axonogenesis; pharyngeal system development; positive regulation of angiogenesis; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of insulin secretion; positive regulation of interferon-gamma production; positive regulation of interleukin-1 alpha production; positive regulation of interleukin-1 beta production; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; sensory system development; spinal cord motor neuron differentiation; trigeminal nerve development; ventricular cardiac muscle morphogenesis
Show more

Research Articles on ISL1

1. ISL-1 is widely expressed in Olfactory neuroblastoma tumors with neuroendocrine differentiation and therefore of limited value in their differential diagnosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ISL1 antibody

 Reference Product  PubMed Publications
 NKX2-5 antibody  >50 publications with ISL1 and NKX2-5
 NKX6-1 antibody  >26 publications with ISL1 and NKX6-1
 MEF2C antibody  >20 publications with ISL1 and MEF2C
 SHH antibody  >16 publications with ISL1 and SHH
 BMP4 antibody  >12 publications with ISL1 and BMP4
 FGF10 antibody  >10 publications with ISL1 and FGF10
 FGF8 antibody  >9 publications with ISL1 and FGF8
 NKX6-2 antibody  >1 publications with ISL1 and NKX6-2

Pathways associated with anti-ISL1 antibody

 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway antibodies  Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway Diagram

Diseases associated with anti-ISL1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >42 publications with ISL1 and Cardiovascular Diseases
 Congenital Abnormalities Antibodies  >35 publications with ISL1 and Congenital Abnormalities
 Heart Defects, Congenital Antibodies  >27 publications with ISL1 and Heart Defects, Congenital
 Cardiovascular Abnormalities Antibodies  >26 publications with ISL1 and Cardiovascular Abnormalities
 Embryo Loss Antibodies  >15 publications with ISL1 and Embryo Loss
 Nervous System Diseases Antibodies  >13 publications with ISL1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >7 publications with ISL1 and Breast Neoplasms
 Skin Diseases Antibodies  >6 publications with ISL1 and Skin Diseases
 Lung Neoplasms Antibodies  >5 publications with ISL1 and Lung Neoplasms
 Abnormalities, Multiple Antibodies  >5 publications with ISL1 and Abnormalities, Multiple

Organs/Tissues associated with anti-ISL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >140 publications with ISL1 and Embryonic Tissue
 Muscle Antibodies  >126 publications with ISL1 and Muscle
 Brain Antibodies  >70 publications with ISL1 and Brain
 Pancreas Antibodies  >69 publications with ISL1 and Pancreas
 Eye Antibodies  >47 publications with ISL1 and Eye
 Lung Antibodies  >14 publications with ISL1 and Lung
 Intestine Antibodies  >8 publications with ISL1 and Intestine
 Stomach Antibodies  >8 publications with ISL1 and Stomach
 Kidney Antibodies  >3 publications with ISL1 and Kidney
 Testis Antibodies  >3 publications with ISL1 and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000