EPCR (PROCR) ELISA Kit for Mouse

For quantitative detection of activated mouse EPCR in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Endothelial protein C receptor (EPCR), also known as CCCA or PROCR, is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
Principle: Mouse EPCR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for EPCR has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L18-S214) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPCR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse EPCR amount of sample captured in plate.

Small volumes of PROCR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178731 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EPCR (PROCR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PROCR. The ELISA analytical biochemical technique of the MBS178731 kit is based on PROCR antibody-PROCR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PROCR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PROCR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).