Syndecan-3/SDC3 (SDC3) ELISA Kit for Mouse

Principle of the Assay: The Picokine™ Mouse Sdc3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Sdc3 with a 96-well strip plate that is pre-coated with antibody specific for Sdc3. The detection antibody is a biotinylated antibody specific for Sdc3. The kit contains recombinant Mouse Sdc3 with immunogen: Expression system for standard: NSO; Immunogen sequence: A45-V387. The kit is analytically validated with ready to use reagents. To measure Mouse Sdc3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Sdc3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Sdc3 in the sample.

Background/Introduction: Syndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.

Small volumes of Sdc3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178744 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Syndecan-3/SDC3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Sdc3. The ELISA analytical biochemical technique of the MBS178744 kit is based on Sdc3 antibody-Sdc3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sdc3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Sdc3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).