HGFA (HGFAC) ELISA Kit for Human

Principle of the Assay: The Human HGFA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HGFA has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q36-S655) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGFA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HGFA amount of sample captured in plate.

Background: Hepatocyte growth factor activator, also known as HGFA, is a protein that in humans is encoded by the HGFAC gene. This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants.

Small volumes of HGFAC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178772 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HGFA (HGFAC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HGFAC. The ELISA analytical biochemical technique of the MBS178772 kit is based on HGFAC antibody-HGFAC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HGFAC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HGFAC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).