TNFRSF19/TROY (TNFRSF19) ELISA Kit for Rat

Principle of the Assay: The Rat TNFRSF19/TROY ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF19/TROY has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E30-L170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF19/TROY is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat TNFRSF19/TROY amount of sample captured in plate.

Background: Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Small volumes of Tnfrsf19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS178774 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNFRSF19/TROY, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Tnfrsf19. The ELISA analytical biochemical technique of the MBS178774 kit is based on Tnfrsf19 antibody-Tnfrsf19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tnfrsf19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Tnfrsf19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).