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ADH1A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178785

Rabbit IgG polyclonal antibody for Alcohol dehydrogenase 1A(ADH1A) detection.Background: Alcohol dehydrogenase 1A is an enzyme that in humans is encoded by the ADH1A gene. This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is… More details

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Product Overview

Online Datasheet in PDF
Product Name
ADH1A, Polyclonal Antibody
Full Product Name
Anti-ADH1A Antibody
Product Gene Name
anti-ADH1A antibody
Product Synonym Names
ADH; ADH1; ADH1A; Alcohol dehydrogenase 1; Alcohol dehydrogenase 1A; Aldehyde reductase; P07327; alcohol dehydrogenase 1A (class I), alpha polypeptide
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
39,859 Da
OMIM
M12963 mRNA
3D Structure
ModBase 3D Structure for P07327
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E. coli-derived human ADH1A recombinant protein (Position: K213-F375). Human ADH1A shares 87.1% and 82.8% amino acid (aa) sequence identity with mouse and rat ADH1A, respectively.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ADH1A antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-ADH1A antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-ADH1A antibody

Western blot analysis of ADH1A expression in rat lung extract (lane 1), mouse liver extract (lane 2) and HEPG2 whole cell lysates (lane 3). ADH1A at 40KD was detected using rabbit anti- ADH1A Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-ADH1A Polyclonal Antibody – Cat# MBS178785

Immunohistochemistry (IHC) of anti-ADH1A antibody

ADH1A was detected in paraffin-embedded sections of human liver cancer tissues using rabbit anti- ADH1A Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-ADH1A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178785

Product References and Citations for anti-ADH1A antibody

1. Lange LG, Sytkowski AJ, Vallee BL (1976). "Human liver alcohol dehydrogenase: purification, composition, and catalytic features".Biochemistry 15 (21): 4687-93.
2. Smith M (Mar 1986). "Genetics of human alcohol and aldehyde dehydrogenases". Adv Hum Genet 15: 249-90.

Other Notes

Small volumes of anti-ADH1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADH1A purchase

MBS178785 is an antibody targeting ADH1A. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ADH1A. It may not necessarily be applicable to this product.

NCBI GI #
4501929
NCBI GeneID
124
NCBI Accession #
NP_000658.1
UniProt Primary Accession #
P07327
UniProt Secondary Accession #
Q17R68; A8K3E3
UniProt Related Accession #
P07327
GenBank Nucleotide #
NM_000667.3
NCBI Official Full Name
alcohol dehydrogenase 1A
NCBI Official Synonym Full Names
alcohol dehydrogenase 1A (class I), alpha polypeptide
NCBI Official Symbol
ADH1A
NCBI Official Synonym Symbols
ADH1
UniProt Protein Name
Alcohol dehydrogenase 1A
UniProt Synonym Protein Names
Alcohol dehydrogenase subunit alpha
UniProt Gene Name
ADH1A
UniProt Synonym Gene Names
ADH1
NCBI Protein Information
alcohol dehydrogenase 1A

NCBI Summary for ADH1A

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]
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UniProt Comments for ADH1A

ADH1A: a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Protein type: Amino Acid Metabolism - tyrosine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.1; Lipid Metabolism - fatty acid; Oxidoreductase; Transcription regulation; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; protein binding; retinol dehydrogenase activity

Biological Process: drug metabolic process; ethanol oxidation
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Research Articles on ADH1A

1. Data indicate that the up-regulation of aldehyde dehydrogenase-1 (ALDH1) after neoadjuvant chemotherapy (NAC) predicts poor survival in locally advanced breast cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADH1A antibody

 Reference Product  PubMed Publications
 ALDH1A1 antibody  >5 publications with ADH1A and ALDH1A1
 GSTP1 antibody  >2 publications with ADH1A and GSTP1
 ALDH3B2 antibody  >1 publications with ADH1A and ALDH3B2

Pathways associated with anti-ADH1A antibody

 Products by Pathway  Pathway Diagram
 Abacavir Metabolism Pathway antibodies  Abacavir Metabolism Pathway Diagram
 Abacavir Transport And Metabolism Pathway antibodies  Abacavir Transport And Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Chemical Carcinogenesis Pathway antibodies  Chemical Carcinogenesis Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Ethanol Oxidation Pathway antibodies  Ethanol Oxidation Pathway Diagram
 Fatty Acid Omega Oxidation Pathway antibodies  Fatty Acid Omega Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram

Diseases associated with anti-ADH1A antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >5 publications with ADH1A and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with ADH1A and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with ADH1A and Liver Neoplasms
 Poisoning Antibodies  >1 publications with ADH1A and Poisoning
 Recurrence Antibodies  >1 publications with ADH1A and Recurrence
 Necrosis Antibodies  >1 publications with ADH1A and Necrosis

Organs/Tissues associated with anti-ADH1A antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >13 publications with ADH1A and Liver
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