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AKR1B1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178791

Rabbit IgG polyclonal antibody for Aldose reductase(AKR1B1) detection.Background: Aldo-keto reductase family 1, member B1 (aldose reductase), also known as AR, is an enzyme that in humans is encoded by the AKR1B1 gene. This gene encodes a member of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
AKR1B1, Polyclonal Antibody
Full Product Name
Anti-AKR1B1 Antibody
Product Gene Name
anti-AKR1B1 antibody
Product Synonym Names
ADR; AKR1B 1; Akr1b1; Aldehyde reductase 1; Aldehyde reductase; Aldose reductase; aldr 1; aldr1; ALR2; AR; P15121; aldo-keto reductase family 1 member B
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
35,853 Da
OMIM
J04795 mRNA
3D Structure
ModBase 3D Structure for P15121
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-AKR1B1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-AKR1B1 antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-AKR1B1 antibody

Western blot analysis of AKR1B1 expression in rat cardiac muscle extract (lane 1), human placenta extract (lane 2) and HELA whole cell lysates (lane 3). AKR1B1 at 36KD was detected using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-AKR1B1 Polyclonal Antibody – Cat# MBS178791

Immunohistochemistry (IHC) of anti-AKR1B1 antibody

AKR1B1 was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-AKR1B1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178791

Immunohistochemistry (IHC) of anti-AKR1B1 antibody

AKR1B1 was detected in paraffin-embedded sections of rat adrenal gland tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-AKR1B1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178791

Immunohistochemistry (IHC) of anti-AKR1B1 antibody

AKR1B1 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-AKR1B1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178791

Product References and Citations for anti-AKR1B1 antibody

1. Ferraretto A, Negri A, Giuliani A, et al. (1993). "Aldose reductase is involved in long-term adaptation of EUE cells to hyperosmotic stress".Biochim. Biophys. Acta 1175(3): 283-8.
2. Graham A, Heath P, Morten JE, Markham AF (March 1991). "The human aldose reductase gene maps to chromosome region 7q35". Hum. Genet. 86 (5): 509-14.

Other Notes

Small volumes of anti-AKR1B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKR1B1 purchase

MBS178791 is an antibody targeting AKR1B1. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AKR1B1. It may not necessarily be applicable to this product.

NCBI GI #
4502049
NCBI GeneID
231
NCBI Accession #
NP_001619.1
UniProt Primary Accession #
P15121
UniProt Secondary Accession #
Q5U031; Q6FGA4; Q6ICP2; Q9BS21; Q9UCI9; B2R8N3
UniProt Related Accession #
P15121
GenBank Nucleotide #
NM_001628.3
NCBI Official Full Name
aldose reductase isoform 1
NCBI Official Synonym Full Names
aldo-keto reductase family 1 member B
NCBI Official Symbol
AKR1B1
NCBI Official Synonym Symbols
AR; ADR; ALR2; ALDR1
UniProt Protein Name
Aldose reductase
UniProt Synonym Protein Names
Aldehyde reductase; Aldo-keto reductase family 1 member B1
Protein Family
Aldose reductase
UniProt Gene Name
AKR1B1
UniProt Synonym Gene Names
ALDR1; AR
NCBI Protein Information
aldose reductase

NCBI Summary for AKR1B1

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
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UniProt Comments for AKR1B1

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: aldehyde reductase activity; aldo-keto reductase activity; electron carrier activity; glyceraldehyde oxidoreductase activity

Biological Process: C21-steroid hormone biosynthetic process; carbohydrate metabolic process; fructose biosynthetic process; response to stress
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Research Articles on AKR1B1

1. -106T allele of AKR1B1 C-106T polymorphism may be associated with increased risk for essential hypertension in Chinese Han population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AKR1B1 antibody

 Reference Product  PubMed Publications
 SORD antibody  >2 publications with AKR1B1 and SORD
 GLO1 antibody  >1 publications with AKR1B1 and GLO1

Pathways associated with anti-AKR1B1 antibody

 Products by Pathway  Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose Biosynthesis Pathway antibodies  Fructose Biosynthesis Pathway Diagram
 Fructose Metabolism Pathway antibodies  Fructose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with anti-AKR1B1 antibody

 Disease Name  Pubmed Publications
 Diabetes Complications Antibodies  >40 publications with AKR1B1 and Diabetes Complications
 Inflammation Antibodies  >18 publications with AKR1B1 and Inflammation
 Cataract Antibodies  >11 publications with AKR1B1 and Cataract
 Hyperglycemia Antibodies  >10 publications with AKR1B1 and Hyperglycemia
 Kidney Diseases Antibodies  >9 publications with AKR1B1 and Kidney Diseases
 Necrosis Antibodies  >9 publications with AKR1B1 and Necrosis
 Disease Models, Animal Antibodies  >8 publications with AKR1B1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >7 publications with AKR1B1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >6 publications with AKR1B1 and Nervous System Diseases
 Adenocarcinoma Antibodies  >5 publications with AKR1B1 and Adenocarcinoma

Organs/Tissues associated with anti-AKR1B1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >27 publications with AKR1B1 and Blood
 Uterus Antibodies  >17 publications with AKR1B1 and Uterus
 Liver Antibodies  >15 publications with AKR1B1 and Liver
 Eye Antibodies  >14 publications with AKR1B1 and Eye
 Lung Antibodies  >11 publications with AKR1B1 and Lung
 Vascular Antibodies  >10 publications with AKR1B1 and Vascular
 Kidney Antibodies  >10 publications with AKR1B1 and Kidney
 Placenta Antibodies  >9 publications with AKR1B1 and Placenta
 Muscle Antibodies  >8 publications with AKR1B1 and Muscle
 Heart Antibodies  >7 publications with AKR1B1 and Heart
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