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CA3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178805

Rabbit IgG polyclonal antibody for Carbonic anhydrase 3(CA3) detection.Background: Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family (at least six separate genes are known) that encode… More details

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Product Overview

Online Datasheet in PDF
Product Name
CA3, Polyclonal Antibody
Full Product Name
Anti-CA3 Antibody
Product Gene Name
anti-CA3 antibody
Product Synonym Names
CA3; CAH3; CAIII; CA-III; Carbonic Anhydrase III; P07451; Carbonic anhydrase 3; carbonic anhydrase III, muscle specific
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
29,557 Da
OMIM
M29458 Genomic DNA
3D Structure
ModBase 3D Structure for P07451
Notes
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E. coli-derived human CA3 recombinant protein (Position: D14-E235). Human CA3 shares 92.3% and 91.4% amino acid (aa) sequence identity with mouse and rat CA3, respectively.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CA3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CA3 antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-CA3 antibody

Western blot analysis of CA3 expression in rat cardiac muscle extract (lane 1) and mouse cardiac muscle extract (lane 2). CA3 at 29KD was detected using rabbit anti- CA3 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-CA3 Polyclonal Antibody – Cat# MBS178805

Immunohistochemistry (IHC) of anti-CA3 antibody

CA3 was detected in paraffin-embedded sections of mouse skeletal muscle tissues using rabbit anti- CA3 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-CA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178805

Immunohistochemistry (IHC) of anti-CA3 antibody

CA3 was detected in paraffin-embedded sections of rat skeletal muscle tissues using rabbit anti- CA3 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-CA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178805

Immunohistochemistry (IHC) of anti-CA3 antibody

CA3 was detected in paraffin-embedded sections of human osteosarcoma tissues using rabbit anti- CA3 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-CA3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178805

Product References and Citations for anti-CA3 antibody

1. Edwards, Y. H., Lloyd, J. C., Parkar, M., Povey, S.The gene for human muscle specific carbonic anhydrase (CAIII) is assigned to chromosome 8.Ann. Hum. Genet. 50: 41-47, 1986.
2. Heath, R., Carter, N. D., Jeffery, S., Edwards, R. J., Watts, D. C., Watts, R. L.Evaluation of carrier detection of Duchenne muscular dystrophy using carbonic anhydrase III and creatine kinase.Am. J. Med. Genet. 21: 291-296, 1985.
3. Wade, R., Gunning, P., Eddy, R., Shows, T., Kedes, L.Nucleotide sequence, tissue-specific expression, and chromosome location of human carbonic anhydrase III: the human CAIII gene is located on the same chromosome as the closely linked CAI and CAII genes.Proc. Nat. Acad. Sci. 83: 9571-9575, 1986.

Other Notes

Small volumes of anti-CA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA3 purchase

MBS178805 is an antibody targeting CA3. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CA3. It may not necessarily be applicable to this product.

NCBI GI #
4885099
NCBI GeneID
761
NCBI Accession #
NP_005172.1
UniProt Primary Accession #
P07451
UniProt Secondary Accession #
O60842; B2R867; B3KUC8
UniProt Related Accession #
P07451
GenBank Nucleotide #
NM_005181.3
NCBI Official Full Name
carbonic anhydrase 3
NCBI Official Synonym Full Names
carbonic anhydrase 3
NCBI Official Symbol
CA3
NCBI Official Synonym Symbols
Car3; CAIII
UniProt Protein Name
Carbonic anhydrase 3
UniProt Synonym Protein Names
Carbonate dehydratase III; Carbonic anhydrase III; CA-III
Protein Family
Carbonic anhydrase
UniProt Gene Name
CA3
UniProt Synonym Gene Names
CA-III
NCBI Protein Information
carbonic anhydrase 3

NCBI Summary for CA3

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]
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UniProt Comments for CA3

CA3: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity

Biological Process: bicarbonate transport
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Research Articles on CA3

1. CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CA3 antibody

 Reference Product  PubMed Publications
 SRC antibody  >28 publications with CA3 and SRC
 INS antibody  >7 publications with CA3 and INS
 CCND1 antibody  >1 publications with CA3 and CCND1

Pathways associated with anti-CA3 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram
 Reversible Hydration Of Carbon Dioxide Pathway antibodies  Reversible Hydration Of Carbon Dioxide Pathway Diagram

Diseases associated with anti-CA3 antibody

 Disease Name  Pubmed Publications
 Seizures Antibodies  >1317 publications with CA3 and Seizures
 Memory Disorders Antibodies  >443 publications with CA3 and Memory Disorders
 Learning Disorders Antibodies  >349 publications with CA3 and Learning Disorders
 Neurobehavioral Manifestations Antibodies  >294 publications with CA3 and Neurobehavioral Manifestations
 Cognition Disorders Antibodies  >210 publications with CA3 and Cognition Disorders
 Necrosis Antibodies  >197 publications with CA3 and Necrosis
 Atrophy Antibodies  >153 publications with CA3 and Atrophy
 Inflammation Antibodies  >130 publications with CA3 and Inflammation
 Prenatal Injuries Antibodies  >113 publications with CA3 and Prenatal Injuries
 Prenatal Exposure Delayed Effects Antibodies  >113 publications with CA3 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with anti-CA3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10202 publications with CA3 and Brain
 Nerve Antibodies  >1920 publications with CA3 and Nerve
 Blood Antibodies  >789 publications with CA3 and Blood
 Embryonic Tissue Antibodies  >97 publications with CA3 and Embryonic Tissue
 Muscle Antibodies  >84 publications with CA3 and Muscle
 Heart Antibodies  >76 publications with CA3 and Heart
 Liver Antibodies  >63 publications with CA3 and Liver
 Eye Antibodies  >48 publications with CA3 and Eye
 Kidney Antibodies  >43 publications with CA3 and Kidney
 Mouth Antibodies  >34 publications with CA3 and Mouth
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