• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LDHA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178831

Rabbit IgG polyclonal antibody for L-lactate dehydrogenase A chain(LDHA) detection.Background: Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LDHA, Polyclonal Antibody
Full Product Name
Anti-LDHA Antibody
Product Gene Name
Product Synonym Names
GSD11; l7R2; LDH A; LDH-A; LDH M; LDH-M; LDH1; ldha; LDHM; PIG19; P00338; L-lactate dehydrogenase A chain; lactate dehydrogenase A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
26,712 Da
OMIM
X02152 mRNA
3D Structure
ModBase 3D Structure for P00338
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E. coli-derived human LDHA recombinant protein (Position: A2-R106). Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-LDHA antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-LDHA antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-LDHA antibody

Western blot analysis of LDHA expression in rat spleen extract (lane 1), ANA-1 whole cell lysates (lane 2) and JURKAT whole cell lysates (lane 3). LDHA at 37KD was detected using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-LDHA Polyclonal Antibody – Cat# MBS178831

Immunohistochemistry (IHC) of anti-LDHA antibody

LDHA was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-LDHA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178831

Immunohistochemistry (IHC) of anti-LDHA antibody

LDHA was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-LDHA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178831

Immunohistochemistry (IHC) of anti-LDHA antibody

LDHA was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- LDHA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-LDHA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178831

Product References and Citations for anti-LDHA antibody

1. Chung FZ, Tsujibo H, Bhattacharyya U, Sharief FS, Li SS (November 1985). "Genomic organization of human lactate dehydrogenase-A gene". Biochem. J. 231 (3): 537-41.
2. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925-7.

Other Notes

Small volumes of anti-LDHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDHA purchase

MBS178831 is an antibody targeting LDHA. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-lactate dehydrogenase A chain isoform 2
NCBI Official Synonym Full Names
lactate dehydrogenase A
NCBI Official Symbol
LDHA
NCBI Official Synonym Symbols
LDHM; GSD11; PIG19; HEL-S-133P
UniProt Protein Name
L-lactate dehydrogenase A chain
UniProt Synonym Protein Names
Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59
UniProt Gene Name
LDHA
UniProt Synonym Gene Names
LDH-A; LDH-M
NCBI Protein Information
L-lactate dehydrogenase A chain

NCBI Summary for LDHA

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000