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RPS27 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS178849

Rabbit IgG polyclonal antibody for 40S ribosomal protein S27(RPS27) detection.Background: 40S ribosomal protein S27, also known as metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. Ribosomes, the organelles that… More details

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Product Overview

Online Datasheet in PDF
Product Name
MPS1 (RPS27), Polyclonal Antibody
Full Product Name
Anti-MPS1 Antibody
Product Gene Name
anti-RPS27 antibody
Product Synonym Names
Metallopan stimulin 1; Metallopan-stimulin 1; Metallopanstimulin 1; Metallopanstimulin1; MPS-1; MPS1; MPS 1; pmn; rps27; S27; P42677; 40S ribosomal protein S27; ribosomal protein S27
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
9,461 Da
OMIM
L19739 mRNA
3D Structure
ModBase 3D Structure for P42677
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E. coli-derived human MPS1 recombinant protein (Position: P2-H84). Human MPS1 shares 100% amino acid (aa) sequence identity with both mouse and rat MPS1.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RPS27 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-RPS27 antibody
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml

Western Blot (WB) of anti-RPS27 antibody

Western blot analysis of MPS1 expression in rat spleen extract (lane 1), mouse liver extract (lane 2) and HELA whole cell lysates (lane 3). MPS1 at 10KD was detected using rabbit anti- MPS1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-RPS27 Polyclonal Antibody – Cat# MBS178849

Immunohistochemistry (IHC) of anti-RPS27 antibody

MPS1 was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- MPS1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-RPS27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178849

Immunohistochemistry (IHC) of anti-RPS27 antibody

MPS1 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- MPS1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-RPS27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178849

Immunohistochemistry (IHC) of anti-RPS27 antibody

MPS1 was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- MPS1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.
Rabbit Anti-RPS27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS178849

Product References and Citations for anti-RPS27 antibody

1. Fernandez-Pol JA (1996). "Metallopanstimulin as a novel tumor marker in sera of patients with various types of common cancers: implications for prevention and therapy". Anticancer Research 16 (4B): 2177-85.
2. Tsui SK, Lee SM, Fung KP, Waye MM, Lee CY (Oct 1996). "Primary structures and sequence analysis of human ribosomal proteins L39 and S27". Biochemistry and Molecular Biology International 40 (3): 611-6.

Other Notes

Small volumes of anti-RPS27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPS27 purchase

MBS178849 is an antibody targeting RPS27. Also known as Anti-MPS1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS27. It may not necessarily be applicable to this product.

NCBI GI #
4506711
NCBI GeneID
6232
NCBI Accession #
NP_001021.1
UniProt Primary Accession #
P42677
UniProt Secondary Accession #
Q5T4L6
UniProt Related Accession #
P42677
GenBank Nucleotide #
NM_001030.4
NCBI Official Full Name
40S ribosomal protein S27
NCBI Official Synonym Full Names
ribosomal protein S27
NCBI Official Symbol
RPS27
NCBI Official Synonym Symbols
S27; MPS1; MPS-1
UniProt Protein Name
40S ribosomal protein S27
UniProt Synonym Protein Names
Metallopan-stimulin 1; MPS-1
Protein Family
40S ribosomal protein
UniProt Gene Name
RPS27
UniProt Synonym Gene Names
MPS1; MPS-1
NCBI Protein Information
40S ribosomal protein S27

NCBI Summary for RPS27

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
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UniProt Comments for RPS27

RPS27: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; rRNA processing; sister chromatid cohesion; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
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Research Articles on RPS27

1. MPS1 is a new marker of Purkinje Cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPS27 antibody

 Reference Product  PubMed Publications
 RPS3A antibody  >2 publications with RPS27 and RPS3A
 RPS28 antibody  >1 publications with RPS27 and RPS28

Pathways associated with anti-RPS27 antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cytoplasmic Ribosomal Proteins Pathway antibodies  Cytoplasmic Ribosomal Proteins Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Eukaryotic Translation Termination Pathway antibodies  Eukaryotic Translation Termination Pathway Diagram
 Formation Of A Pool Of Free 40S Subunits Pathway antibodies  Formation Of A Pool Of Free 40S Subunits Pathway Diagram

Diseases associated with anti-RPS27 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >5 publications with RPS27 and Neoplasm Metastasis
 Fibrosis Antibodies  >2 publications with RPS27 and Fibrosis
 Nervous System Diseases Antibodies  >1 publications with RPS27 and Nervous System Diseases
 Prostatic Diseases Antibodies  >1 publications with RPS27 and Prostatic Diseases
 Neoplasms, Experimental Antibodies  >1 publications with RPS27 and Neoplasms, Experimental
 Heart Diseases Antibodies  >1 publications with RPS27 and Heart Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with RPS27 and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >1 publications with RPS27 and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >1 publications with RPS27 and Liver Neoplasms
 Lung Neoplasms Antibodies  >1 publications with RPS27 and Lung Neoplasms

Organs/Tissues associated with anti-RPS27 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10 publications with RPS27 and Blood
 Brain Antibodies  >6 publications with RPS27 and Brain
 Bone Antibodies  >6 publications with RPS27 and Bone
 Bone Marrow Antibodies  >5 publications with RPS27 and Bone Marrow
 Stomach Antibodies  >3 publications with RPS27 and Stomach
 Lung Antibodies  >2 publications with RPS27 and Lung
 Prostate Antibodies  >2 publications with RPS27 and Prostate
 Mouth Antibodies  >2 publications with RPS27 and Mouth
 Skin Antibodies  >2 publications with RPS27 and Skin
 Adrenal Gland Antibodies  >1 publications with RPS27 and Adrenal Gland
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