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SDF1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179065

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor… More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SDF1, Monoclonal Antibody
Full Product Name
Anti-SDF1 Rabbit Monoclonal Antibody
Product Gene Name
anti-SDF1 antibody
Product Synonym Gene Name
CXCL12
Product Synonym Names
Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF; SDF-1-beta(3-72); SDF-1-alpha(3-67); CXCL12; SDF1; SDF1A; SDF1B
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
10666 MW
OMIM
L36033 mRNA
3D Structure
ModBase 3D Structure for P48061
Immunogen
A synthesized peptide derived from human SDF1
Subcellular Localization
Secreted.
Tissue Specificity
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-SDF1 antibody

Immunology; Innate Immunity; Chemokines; Alpha Chemokines (CXC); Microbiology; Interspecies Interaction; Host Virus Interaction; Kits/Lysates/Other; ELISA Kits; cytokines and cytokine Receptors ELISA Kits
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SDF1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-SDF1 antibody
WB: 1:1000-1:5000
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-SDF1 antibody

Western blot analysis of SDF1 expression in Jurkat cell lysate (MBS179065).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL12 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCL12
Rabbit Anti-SDF1 Monoclonal Antibody – Cat# MBS179065

Other Notes

Small volumes of anti-SDF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SDF1 purchase

MBS179065 is an antibody targeting SDF1. Also known as Anti-SDF1 Rabbit Monoclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SDF1. It may not necessarily be applicable to this product.

NCBI GI #
10834988
NCBI GeneID
6387
NCBI Accession #
NP_000600.1
UniProt Primary Accession #
P48061
UniProt Secondary Accession #
Q2L985; Q2L986; Q2L988; Q5IT36; Q6ICW0; Q9H554; B2R4G0; E7EVL0; H7BYN8
UniProt Related Accession #
P48061
GenBank Nucleotide #
NM_000609.6
NCBI Official Full Name
stromal cell-derived factor 1 isoform beta
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 12
NCBI Official Symbol
CXCL12
NCBI Official Synonym Symbols
IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
UniProt Protein Name
Stromal cell-derived factor 1
UniProt Synonym Protein Names
C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH
UniProt Gene Name
CXCL12
UniProt Synonym Gene Names
SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF
NCBI Protein Information
stromal cell-derived factor 1

NCBI Summary for SDF1

This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
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UniProt Comments for SDF1

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
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Research Articles on SDF1

1. Results show that CXCL12 is highly expressed in metastatic lymph node (MNL) of non-small cell lung neoplasm (NSCLC) and is associated with poor prognosis which suggest that CXCL12 may play a role in tumor progression of NSCLC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SDF1 antibody

 Reference Product  PubMed Publications
 CXCR4 antibody  >2872 publications with SDF1 and CXCR4
 CCR5 antibody  >278 publications with SDF1 and CCR5
 CXCR7 antibody  >193 publications with SDF1 and CXCR7
 CXCR3 antibody  >114 publications with SDF1 and CXCR3
 HIF1A antibody  >68 publications with SDF1 and HIF1A
 CCR4 antibody  >40 publications with SDF1 and CCR4
 STAT3 antibody  >30 publications with SDF1 and STAT3
 JAK2 antibody  >16 publications with SDF1 and JAK2
 VAV1 antibody  >14 publications with SDF1 and VAV1
 SDC4 antibody  >5 publications with SDF1 and SDC4

Pathways associated with anti-SDF1 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram

Diseases associated with anti-SDF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1293 publications with SDF1 and Neoplasms
 Cardiovascular Diseases Antibodies  >483 publications with SDF1 and Cardiovascular Diseases
 Inflammation Antibodies  >464 publications with SDF1 and Inflammation
 Neoplasm Metastasis Antibodies  >325 publications with SDF1 and Neoplasm Metastasis
 HIV Infections Antibodies  >256 publications with SDF1 and HIV Infections
 Breast Neoplasms Antibodies  >230 publications with SDF1 and Breast Neoplasms
 Heart Diseases Antibodies  >224 publications with SDF1 and Heart Diseases
 Neoplasm Invasiveness Antibodies  >186 publications with SDF1 and Neoplasm Invasiveness
 Necrosis Antibodies  >185 publications with SDF1 and Necrosis
 Myocardial Infarction Antibodies  >174 publications with SDF1 and Myocardial Infarction

Organs/Tissues associated with anti-SDF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1270 publications with SDF1 and Bone
 Bone Marrow Antibodies  >1174 publications with SDF1 and Bone Marrow
 Vascular Antibodies  >938 publications with SDF1 and Vascular
 Brain Antibodies  >415 publications with SDF1 and Brain
 Muscle Antibodies  >312 publications with SDF1 and Muscle
 Lung Antibodies  >277 publications with SDF1 and Lung
 Heart Antibodies  >250 publications with SDF1 and Heart
 Liver Antibodies  >228 publications with SDF1 and Liver
 Embryonic Tissue Antibodies  >187 publications with SDF1 and Embryonic Tissue
 Nerve Antibodies  >151 publications with SDF1 and Nerve
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